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The map label for this gene is nemA [H]

Identifier: 162146064

GI number: 162146064

Start: 244222

End: 245286

Strand: Direct

Name: nemA [H]

Synonym: GDI_0232

Alternate gene names: 162146064

Gene position: 244222-245286 (Clockwise)

Preceding gene: 162146063

Following gene: 162146065

Centisome position: 6.19

GC content: 68.08

Gene sequence:

>1065_bases
TTGGCTTCTCTGTTTACGCCCCTGCGGGTCGGTGACCTGACATTGCCCAACCGGGTCATCATGGCGCCGCTGACACGCCA
GCGCGCCGGCGAGACGCATATCCCCAACGACCTGATGGCCACCTACTACGTCCAGCGGTCGCTGGCGGGCCTGATCCTGT
CGGAAGCGACGCCGGTGTCCCCGCAGGGCATCGGCTATGCCGGGGTCCCGGGCATCTGGCTGCCCGAGCATGTCGAGGGC
TGGAAGAAGGTCACGTCCGCCGTGCATGCGGTCGGGGGGCGCATCTTCCTCCAGCTCTGGCACGTGGGCCGGATTTCCGA
CCCCGTGTTCCTCGATGGCGACCTGCCCGTCGCGCCCAGCGCCATCGCGGCCAGGGGTCATGTCAGCCAGCTCCGCCCCG
AACGCCCTTACGTGACGCCCCGCGCGCTGGAAACGCACGAGATCCCCGGGATCGTCACGGCCTTCCGGCACGGGGCCGAA
CTGGCGAAGGAAGCAAATTTCGATGGCGTCGAGATCCACGGCGCGAACGGCTATCTGCTCGACCAGTTCCTGCAGGATTC
CACCAACCACCGCACGGACCGGTACGGCGGCTCGATCGAGAACCGGGCGCGCCTGATGCTGGACGTGACCGACGCCGCCA
TCGACGTCTGGGGCCCGGGGCGCGTGGGCATGCATCTGGCACCCCGGTGCGACGCGCACGACATGGGTGACAGCAATCCG
GCGGCCACTTTCGGCTATGTCGCGACCGAACTCGGGCGGCGCGGCATCGCCTTCATCTGCGCGCGCGAGGCCGAGGGGCC
GGACAGCCTGGGCCCGCAACTGAAGAAGGCATTCGGTGGCGTCTATATCGCCAACGAGGCCTTCACCGGTGCATCCGCCG
AGGCCGCCATCGAAGAGGGCCGGGCCGACGCGGTGGCCTTCGGGAAGACTTTCATCGCCAACCCCGACCTGCCCGAGCGA
CTGAAAATCGGTGCGGACCTGACGCCGCCCGATGCCTCCACGTTCTATGGCGAGGGCGCGCGGGGATATACGGACTATCC
CACGCTGGGCGAATCCGGTGACTGA

Upstream 100 bases:

>100_bases
GAGCTGAGCATCGCCCCGACAGGGGCGGGCGCCTGCTTGCAGCGCGGGAAGGGCAGGCGCATGCTTTTTCAGGATTTCAT
ATCTGGCCGGGAGATGACTA

Downstream 100 bases:

>100_bases
CAGCGGATCTTTCCGAGGGGACACCATGAGCGCTCATCATGTCCTGATCGCCGGTGCCAGCGGCCCGGTCCGTCTGGGCG
CAGGTCGCGGCCCGGCATGG

Product: flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MASLFTPLRVGDLTLPNRVIMAPLTRQRAGETHIPNDLMATYYVQRSLAGLILSEATPVSPQGIGYAGVPGIWLPEHVEG
WKKVTSAVHAVGGRIFLQLWHVGRISDPVFLDGDLPVAPSAIAARGHVSQLRPERPYVTPRALETHEIPGIVTAFRHGAE
LAKEANFDGVEIHGANGYLLDQFLQDSTNHRTDRYGGSIENRARLMLDVTDAAIDVWGPGRVGMHLAPRCDAHDMGDSNP
AATFGYVATELGRRGIAFICAREAEGPDSLGPQLKKAFGGVYIANEAFTGASAEAAIEEGRADAVAFGKTFIANPDLPER
LKIGADLTPPDASTFYGEGARGYTDYPTLGESGD

Sequences:

>Translated_354_residues
MASLFTPLRVGDLTLPNRVIMAPLTRQRAGETHIPNDLMATYYVQRSLAGLILSEATPVSPQGIGYAGVPGIWLPEHVEG
WKKVTSAVHAVGGRIFLQLWHVGRISDPVFLDGDLPVAPSAIAARGHVSQLRPERPYVTPRALETHEIPGIVTAFRHGAE
LAKEANFDGVEIHGANGYLLDQFLQDSTNHRTDRYGGSIENRARLMLDVTDAAIDVWGPGRVGMHLAPRCDAHDMGDSNP
AATFGYVATELGRRGIAFICAREAEGPDSLGPQLKKAFGGVYIANEAFTGASAEAAIEEGRADAVAFGKTFIANPDLPER
LKIGADLTPPDASTFYGEGARGYTDYPTLGESGD
>Mature_353_residues
ASLFTPLRVGDLTLPNRVIMAPLTRQRAGETHIPNDLMATYYVQRSLAGLILSEATPVSPQGIGYAGVPGIWLPEHVEGW
KKVTSAVHAVGGRIFLQLWHVGRISDPVFLDGDLPVAPSAIAARGHVSQLRPERPYVTPRALETHEIPGIVTAFRHGAEL
AKEANFDGVEIHGANGYLLDQFLQDSTNHRTDRYGGSIENRARLMLDVTDAAIDVWGPGRVGMHLAPRCDAHDMGDSNPA
ATFGYVATELGRRGIAFICAREAEGPDSLGPQLKKAFGGVYIANEAFTGASAEAAIEEGRADAVAFGKTFIANPDLPERL
KIGADLTPPDASTFYGEGARGYTDYPTLGESGD

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=49.171270718232, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1789463, Length=214, Percent_Identity=35.5140186915888, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17565138, Length=300, Percent_Identity=28.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=345, Percent_Identity=28.4057971014493, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=349, Percent_Identity=26.9340974212034, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=310, Percent_Identity=27.4193548387097, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=346, Percent_Identity=26.3005780346821, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=345, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=355, Percent_Identity=24.7887323943662, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=383, Percent_Identity=36.8146214099217, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321973, Length=378, Percent_Identity=34.6560846560847, Blast_Score=195, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37907; Mature: 37776

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLFTPLRVGDLTLPNRVIMAPLTRQRAGETHIPNDLMATYYVQRSLAGLILSEATPVS
CCCCCCCCEECCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PQGIGYAGVPGIWLPEHVEGWKKVTSAVHAVGGRIFLQLWHVGRISDPVFLDGDLPVAPS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCEEECCCCCCCCH
AIAARGHVSQLRPERPYVTPRALETHEIPGIVTAFRHGAELAKEANFDGVEIHGANGYLL
HHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHH
DQFLQDSTNHRTDRYGGSIENRARLMLDVTDAAIDVWGPGRVGMHLAPRCDAHDMGDSNP
HHHHHCCCCCCCHHCCCCCCCCEEEEEEEECCEEEECCCCCCCCEECCCCCCCCCCCCCC
AATFGYVATELGRRGIAFICAREAEGPDSLGPQLKKAFGGVYIANEAFTGASAEAAIEEG
CHHHHHHHHHHCCCCEEEEEEECCCCCHHCCHHHHHHHCCEEEECCCCCCCCHHHHHHCC
RADAVAFGKTFIANPDLPERLKIGADLTPPDASTFYGEGARGYTDYPTLGESGD
CCCEEEECCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASLFTPLRVGDLTLPNRVIMAPLTRQRAGETHIPNDLMATYYVQRSLAGLILSEATPVS
CCCCCCCEECCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PQGIGYAGVPGIWLPEHVEGWKKVTSAVHAVGGRIFLQLWHVGRISDPVFLDGDLPVAPS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCEEECCCCCCCCH
AIAARGHVSQLRPERPYVTPRALETHEIPGIVTAFRHGAELAKEANFDGVEIHGANGYLL
HHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHH
DQFLQDSTNHRTDRYGGSIENRARLMLDVTDAAIDVWGPGRVGMHLAPRCDAHDMGDSNP
HHHHHCCCCCCCHHCCCCCCCCEEEEEEEECCEEEECCCCCCCCEECCCCCCCCCCCCCC
AATFGYVATELGRRGIAFICAREAEGPDSLGPQLKKAFGGVYIANEAFTGASAEAAIEEG
CHHHHHHHHHHCCCCEEEEEEECCCCCHHCCHHHHHHHCCEEEECCCCCCCCHHHHHHCC
RADAVAFGKTFIANPDLPERLKIGADLTPPDASTFYGEGARGYTDYPTLGESGD
CCCEEEECCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]