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The map label for this gene is yafC [H]

Identifier: 162146047

GI number: 162146047

Start: 232048

End: 232965

Strand: Direct

Name: yafC [H]

Synonym: GDI_0215

Alternate gene names: 162146047

Gene position: 232048-232965 (Clockwise)

Preceding gene: 162146043

Following gene: 162146049

Centisome position: 5.88

GC content: 66.78

Gene sequence:

>918_bases
ATGGAACAATCGCATGTCTCGCTCGACCGGATGCGGACGTTCGTCCGCATTGCCGAACGCGGCAATCTCGCGGCGGTCGC
CCGGGAAACCGGCGCAGGCCAATCCACGATCACCCGCCATCTGCGCGAGCTTGAAGAGGCGCTGGGTGTCTCGCTGCTCA
CCCGCACCACCCGCCGCACGGCGCTGACCGAAGAGGGCACGCGCTATTACGCGCAATGCCTGAACATCCTGTGCCTGGTC
GAACAGGCCAGCCAGGAAATGCGGCAGACACGCCAGGCAACAGCGGGCACGGTCCGCATCTCCTGCACCGCGGCGCTCGG
CGTCCTGCATGTCAGCCGGCTGATCTTTGCCTTCCAGGACCAGAATCCCGGGATACGGGTGGAATTCAGCCTGACCGACG
AACGTGTCGACCTGGTGCGCGAGGGCGTCGACATCGCCATCCGCCTCGGGCCGCTGACCGATAGTTCGATGAAGCTGCGG
GCGCTGGGTGAAAGCCAGCGCGTGCTGGTGGCCGCGCCGGACTGGCTGCATCGCAACGGCCCACTGAAGCGGCCCGAGGA
TCTGCGCGATCGGGAAGGCGTCCGCCTCTCCCGGGTTCACGGTTCCGACCGGCTGGTCCTCCAGGCTCCGAAGGGCAAGC
ATCACGTCGTTCCGTTCCACGGTCGCCTGAATGTCGATCATGGACTCGCGGCGCGAGAGGCTTTCCTGGCGGGGCGCGGT
TTCGGCCCGGCGCATCGGTGGCTGGTGGAGGACTGCCTACAGGACGGCCGGCTGGACCTCGTGCTGCCGAACTACAGGCT
TCCTCCGGCTCCCCTGAGCATGCTGATCGCACCGGAACGGGCGAGCCTGACGCGGGTGCGTCTCTGCGCCGATTTTCTCG
GCAGCGAAATCGCGAAAATACCCGGCATCGGGCGGTGA

Upstream 100 bases:

>100_bases
GGCTTCCTCATGCAGGCGGAAAGAATGGGCGACTTTCCGAGGATAGGGGATAGGCTATTGTCTTCCCATATGGCCTGAGC
GGAAGGGACAGTGCCGGAAA

Downstream 100 bases:

>100_bases
CGGCGGGCCGGATCAGATACCAGTAGGGCGCGAATTATCCCGCCTGCTGCACGATCCATTGTTCCAGCGCCTCGACCTGT
TCCGTCACGTCCATCCCCCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEQSHVSLDRMRTFVRIAERGNLAAVARETGAGQSTITRHLRELEEALGVSLLTRTTRRTALTEEGTRYYAQCLNILCLV
EQASQEMRQTRQATAGTVRISCTAALGVLHVSRLIFAFQDQNPGIRVEFSLTDERVDLVREGVDIAIRLGPLTDSSMKLR
ALGESQRVLVAAPDWLHRNGPLKRPEDLRDREGVRLSRVHGSDRLVLQAPKGKHHVVPFHGRLNVDHGLAAREAFLAGRG
FGPAHRWLVEDCLQDGRLDLVLPNYRLPPAPLSMLIAPERASLTRVRLCADFLGSEIAKIPGIGR

Sequences:

>Translated_305_residues
MEQSHVSLDRMRTFVRIAERGNLAAVARETGAGQSTITRHLRELEEALGVSLLTRTTRRTALTEEGTRYYAQCLNILCLV
EQASQEMRQTRQATAGTVRISCTAALGVLHVSRLIFAFQDQNPGIRVEFSLTDERVDLVREGVDIAIRLGPLTDSSMKLR
ALGESQRVLVAAPDWLHRNGPLKRPEDLRDREGVRLSRVHGSDRLVLQAPKGKHHVVPFHGRLNVDHGLAAREAFLAGRG
FGPAHRWLVEDCLQDGRLDLVLPNYRLPPAPLSMLIAPERASLTRVRLCADFLGSEIAKIPGIGR
>Mature_305_residues
MEQSHVSLDRMRTFVRIAERGNLAAVARETGAGQSTITRHLRELEEALGVSLLTRTTRRTALTEEGTRYYAQCLNILCLV
EQASQEMRQTRQATAGTVRISCTAALGVLHVSRLIFAFQDQNPGIRVEFSLTDERVDLVREGVDIAIRLGPLTDSSMKLR
ALGESQRVLVAAPDWLHRNGPLKRPEDLRDREGVRLSRVHGSDRLVLQAPKGKHHVVPFHGRLNVDHGLAAREAFLAGRG
FGPAHRWLVEDCLQDGRLDLVLPNYRLPPAPLSMLIAPERASLTRVRLCADFLGSEIAKIPGIGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=29.4915254237288, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=31.740614334471, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=27.9863481228669, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693193, Length=276, Percent_Identity=31.5217391304348, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=26.3888888888889, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787128, Length=273, Percent_Identity=27.8388278388278, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=25.1968503937008, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787589, Length=189, Percent_Identity=26.984126984127, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=275, Percent_Identity=25.8181818181818, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788706, Length=173, Percent_Identity=24.2774566473988, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=176, Percent_Identity=25, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33888; Mature: 33888

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSHVSLDRMRTFVRIAERGNLAAVARETGAGQSTITRHLRELEEALGVSLLTRTTRRT
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
ALTEEGTRYYAQCLNILCLVEQASQEMRQTRQATAGTVRISCTAALGVLHVSRLIFAFQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHEEEC
QNPGIRVEFSLTDERVDLVREGVDIAIRLGPLTDSSMKLRALGESQRVLVAAPDWLHRNG
CCCCEEEEEEECHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCEEEEECCHHHHCCC
PLKRPEDLRDREGVRLSRVHGSDRLVLQAPKGKHHVVPFHGRLNVDHGLAAREAFLAGRG
CCCCCCCCHHCCCCEEEEECCCCEEEEECCCCCCEEEEECCEECCCCCHHHHHHHHHCCC
FGPAHRWLVEDCLQDGRLDLVLPNYRLPPAPLSMLIAPERASLTRVRLCADFLGSEIAKI
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHC
PGIGR
CCCCC
>Mature Secondary Structure
MEQSHVSLDRMRTFVRIAERGNLAAVARETGAGQSTITRHLRELEEALGVSLLTRTTRRT
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
ALTEEGTRYYAQCLNILCLVEQASQEMRQTRQATAGTVRISCTAALGVLHVSRLIFAFQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHEEEC
QNPGIRVEFSLTDERVDLVREGVDIAIRLGPLTDSSMKLRALGESQRVLVAAPDWLHRNG
CCCCEEEEEEECHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCEEEEECCHHHHCCC
PLKRPEDLRDREGVRLSRVHGSDRLVLQAPKGKHHVVPFHGRLNVDHGLAAREAFLAGRG
CCCCCCCCHHCCCCEEEEECCCCEEEEECCCCCCEEEEECCEECCCCCHHHHHHHHHCCC
FGPAHRWLVEDCLQDGRLDLVLPNYRLPPAPLSMLIAPERASLTRVRLCADFLGSEIAKI
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHC
PGIGR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]