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The map label for this gene is shiA [H]

Identifier: 162146024

GI number: 162146024

Start: 206689

End: 208017

Strand: Direct

Name: shiA [H]

Synonym: GDI_0192

Alternate gene names: 162146024

Gene position: 206689-208017 (Clockwise)

Preceding gene: 162146023

Following gene: 162146025

Centisome position: 5.24

GC content: 62.53

Gene sequence:

>1329_bases
ATGAAAAATGTGACGCCACGGCAGGCTTCGTTCCAGGAGGACCAGAATCCGCAGATCATGCGGGCGCGGCTGTCACAATG
GCGGATCGCGTTTGCCAGCATCGTGGGGACGATGCTGGAATGGTATGATTTCACCATCTACAATACCATGGCGGCCCTGA
TTTTCGGTCGCCTCTTCTTCCCCTCCGCGCAGCCGCGTGTGGGCATGATCCTCGCATTCTCGACATATGCCGTGGGCTAC
GTTTCGCGCCCGGTCGGAGCGGTCGTGTTCGGCTGGATCGGGGATCGATATGGTCGCAGGACGGTCCTGGTATTCGCCCT
GACCCTGATGGGGGCCTGTACTGCCAGCATTGCCGTGTTGCCGACATTCGAATCGATCGGGATCGTCGCCCCGCTGGGCC
TCAGCATCGTCCGCTTTCTGCAGGGGCTCGCATTGGGCGGGGAGTGGGCGGGGGCGGTTCTGATCGCCACGGAACACGGA
AGTGCGCACCGTCAGGGGCGGAATTCATCCTGGGCGCAGATGGGGCCGGCGGCGGGGATATTGCTGGCGACGTTCGTCAT
CGGTGGCGTGGGGATTTTCATGCCCGACAGGGCTTTCCTGTCCTGGGGCTGGCGTGTTCCCTTTGCCCTGAGCGCGTTCC
TGATGGTGTTCGGCCTTTGGGTCCGTCGCGCCGTTCCCGAGACGCCGGTGTTCGCCAAGAGCCGCCACGAGGCCGGCGCG
CCCCCCCTGGTCGAAGTGCTGCGCAAGCATAAGGCCGCGCTGGCTATCGCATCGTCTTCGAGAATCGGGACGGACGTCCT
CTATTCGCTCCTGCTCATTTTCAGCATTTCCTACATAACGCAGTTTCTCGATATGCCCCGTTCCGTCGCGCTGGGCTGCA
CGCTGACAGGCAGCGCCTGCGAACTTGTCGCGATTCCGGTCTTCGGCTCTCTGATCGACCGGATAGGGTGCCGGAAAGTC
TATGCAGGCGGCATCCTGGCCGGCTCGCTCATCACGCTAGCGTTCTTTCCGGTATTCGGCATGAAACTGCCCCTGGCGAT
CTTCGGCCTGACGGCGATCGGGACGGTGTCCCATGCGGCGATGTATGCGGCGCAGGGCACATTCATTACCAATCAGTTTC
CTTCCACCGTGCGCTATACCGGGTCGTCCCTGGCTTACACGCTTGGCAGCCTGGCAGGCGGCAGTGCATTCGCCCCGCTG
ATCATGAGCAGCATTGTGGGCGGATGGACAGGCACCTGGGGGATTACACTTTACGTCATGGTGACCCTGGCAGTCACCGC
GGCCGGCATTGTCGCCGCGGGGCACAGGACACCCTCCGAACAGTCATAG

Upstream 100 bases:

>100_bases
CCGCCGGGGCGCTGTGAAGGCGCCCCGGGAACTGAACGCTGCAAAGGTCGCGTGGACGACACCGGCACGGGACGGCTCTG
TTTTCAGGAAACGGTCATTG

Downstream 100 bases:

>100_bases
CCGGTTACTCTGTATCCTCTTGAGATCGGATAAAAGAATGTTTGTCCTCAATCCTTTGCCTCCTGCCGTGGCGGCCAGCG
ACCTCGCGCTCCTGATCCAG

Product: sugar transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKNVTPRQASFQEDQNPQIMRARLSQWRIAFASIVGTMLEWYDFTIYNTMAALIFGRLFFPSAQPRVGMILAFSTYAVGY
VSRPVGAVVFGWIGDRYGRRTVLVFALTLMGACTASIAVLPTFESIGIVAPLGLSIVRFLQGLALGGEWAGAVLIATEHG
SAHRQGRNSSWAQMGPAAGILLATFVIGGVGIFMPDRAFLSWGWRVPFALSAFLMVFGLWVRRAVPETPVFAKSRHEAGA
PPLVEVLRKHKAALAIASSSRIGTDVLYSLLLIFSISYITQFLDMPRSVALGCTLTGSACELVAIPVFGSLIDRIGCRKV
YAGGILAGSLITLAFFPVFGMKLPLAIFGLTAIGTVSHAAMYAAQGTFITNQFPSTVRYTGSSLAYTLGSLAGGSAFAPL
IMSSIVGGWTGTWGITLYVMVTLAVTAAGIVAAGHRTPSEQS

Sequences:

>Translated_442_residues
MKNVTPRQASFQEDQNPQIMRARLSQWRIAFASIVGTMLEWYDFTIYNTMAALIFGRLFFPSAQPRVGMILAFSTYAVGY
VSRPVGAVVFGWIGDRYGRRTVLVFALTLMGACTASIAVLPTFESIGIVAPLGLSIVRFLQGLALGGEWAGAVLIATEHG
SAHRQGRNSSWAQMGPAAGILLATFVIGGVGIFMPDRAFLSWGWRVPFALSAFLMVFGLWVRRAVPETPVFAKSRHEAGA
PPLVEVLRKHKAALAIASSSRIGTDVLYSLLLIFSISYITQFLDMPRSVALGCTLTGSACELVAIPVFGSLIDRIGCRKV
YAGGILAGSLITLAFFPVFGMKLPLAIFGLTAIGTVSHAAMYAAQGTFITNQFPSTVRYTGSSLAYTLGSLAGGSAFAPL
IMSSIVGGWTGTWGITLYVMVTLAVTAAGIVAAGHRTPSEQS
>Mature_442_residues
MKNVTPRQASFQEDQNPQIMRARLSQWRIAFASIVGTMLEWYDFTIYNTMAALIFGRLFFPSAQPRVGMILAFSTYAVGY
VSRPVGAVVFGWIGDRYGRRTVLVFALTLMGACTASIAVLPTFESIGIVAPLGLSIVRFLQGLALGGEWAGAVLIATEHG
SAHRQGRNSSWAQMGPAAGILLATFVIGGVGIFMPDRAFLSWGWRVPFALSAFLMVFGLWVRRAVPETPVFAKSRHEAGA
PPLVEVLRKHKAALAIASSSRIGTDVLYSLLLIFSISYITQFLDMPRSVALGCTLTGSACELVAIPVFGSLIDRIGCRKV
YAGGILAGSLITLAFFPVFGMKLPLAIFGLTAIGTVSHAAMYAAQGTFITNQFPSTVRYTGSSLAYTLGSLAGGSAFAPL
IMSSIVGGWTGTWGITLYVMVTLAVTAAGIVAAGHRTPSEQS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=424, Percent_Identity=33.9622641509434, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1789941, Length=404, Percent_Identity=34.9009900990099, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1790550, Length=406, Percent_Identity=29.3103448275862, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1788942, Length=425, Percent_Identity=29.1764705882353, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI87082404, Length=449, Percent_Identity=23.3853006681514, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082231, Length=453, Percent_Identity=23.6203090507726, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47103; Mature: 47103

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVTPRQASFQEDQNPQIMRARLSQWRIAFASIVGTMLEWYDFTIYNTMAALIFGRLFF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSAQPRVGMILAFSTYAVGYVSRPVGAVVFGWIGDRYGRRTVLVFALTLMGACTASIAVL
CCCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEE
PTFESIGIVAPLGLSIVRFLQGLALGGEWAGAVLIATEHGSAHRQGRNSSWAQMGPAAGI
ECHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCHHHCCCHHHH
LLATFVIGGVGIFMPDRAFLSWGWRVPFALSAFLMVFGLWVRRAVPETPVFAKSRHEAGA
HHHHHHHCCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PPLVEVLRKHKAALAIASSSRIGTDVLYSLLLIFSISYITQFLDMPRSVALGCTLTGSAC
CHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCC
ELVAIPVFGSLIDRIGCRKVYAGGILAGSLITLAFFPVFGMKLPLAIFGLTAIGTVSHAA
CEEEHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYAAQGTFITNQFPSTVRYTGSSLAYTLGSLAGGSAFAPLIMSSIVGGWTGTWGITLYVM
HHHHCCCEEECCCCCCCEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
VTLAVTAAGIVAAGHRTPSEQS
HHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKNVTPRQASFQEDQNPQIMRARLSQWRIAFASIVGTMLEWYDFTIYNTMAALIFGRLFF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSAQPRVGMILAFSTYAVGYVSRPVGAVVFGWIGDRYGRRTVLVFALTLMGACTASIAVL
CCCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEE
PTFESIGIVAPLGLSIVRFLQGLALGGEWAGAVLIATEHGSAHRQGRNSSWAQMGPAAGI
ECHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCHHHCCCHHHH
LLATFVIGGVGIFMPDRAFLSWGWRVPFALSAFLMVFGLWVRRAVPETPVFAKSRHEAGA
HHHHHHHCCCHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PPLVEVLRKHKAALAIASSSRIGTDVLYSLLLIFSISYITQFLDMPRSVALGCTLTGSAC
CHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCC
ELVAIPVFGSLIDRIGCRKVYAGGILAGSLITLAFFPVFGMKLPLAIFGLTAIGTVSHAA
CEEEHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYAAQGTFITNQFPSTVRYTGSSLAYTLGSLAGGSAFAPLIMSSIVGGWTGTWGITLYVM
HHHHCCCEEECCCCCCCEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
VTLAVTAAGIVAAGHRTPSEQS
HHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]