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The map label for this gene is fadH [C]

Identifier: 162145919

GI number: 162145919

Start: 95770

End: 97077

Strand: Reverse

Name: fadH [C]

Synonym: GDI_0083

Alternate gene names: 162145919

Gene position: 97077-95770 (Counterclockwise)

Preceding gene: 162145921

Following gene: 162145918

Centisome position: 2.46

GC content: 67.13

Gene sequence:

>1308_bases
ATGTCCCTGTTTTCCCCTCTGACCCTGCCCAATGGCACCGTCATCACCAATCGTATCGCCAAGGCCGCCATGGAAGAGAA
CATGGCAGACGCCGACCACGCGCCATCCGAAGGGCTGATCCGTCTCTACCGGGCCTGGGCGGAGGGCGGCGCGGGCCTGA
TCATCACCGGCAACGTGATGGTGGATGCACGGGCCATGACCGGTCCGGCTGGGGTGGTGCTTCAGGACGCGCAGCATCTC
GACCGTTTTCAGGACTGGGCCACGACAGCCCGGTCTGGTGGCGGCCAGATCTGGATGCAGATCAATCATCCGGGGCGGCA
GATGCCTGCCAGCATGGCGCAGGAAACGCTTGCACCGTCTGCTATCCCTCTCGATCTGGGCCGGCAGTCGAAGATGTTCC
CTGTTCCACGTGCCATGAGGGCGGACGATATCGCCGATGTCACCCGCCGCTTCATCCACACGGCCGGCCTGGCCGAGCGT
GCGGGCTTCGATGGGGTCGAGATTCATGCGGCGCATGGCTACCTGCTCAGCCAGTTTCTCTCCCCGCTGAGCAACCGGCG
CACGGACCAGTGGGGCGGCAGCCTCGAAAACCGCGCTCGCCTGCTGCTCGATATCGTGCGGGGCGTGCGGTCGTCCCTGC
GACCGGATTTCGCGGTATCGGTGAAACTCAACTCGGCCGATTTCCAGCGCGGCGGCTTCTCGCCCGACGACGCCCGAGCC
GTCGTCCGGATGCTCGACGGTCTCGGCGTCGATCTCGTGGAACTGTCCGGCGGCAGTTACGAAGCGCCCGCGATGATGGG
GTCCTCTCGCGACGAACGCACCCTGGCGCGGGAAGCCTATTTCCTCGATTTCGCCAAAGACATCGCCACCGTCGCCACGA
TGCCGCTCATGGTGACCGGCGGCATCCGGCGGCAGGACGTGGCAGAGCGCGTCGTGGCCGACGGCATCGCGATGGCAGGT
ATGGCGACGGCGCTGGCGATCGAACCCGCCCTGCCGCGCCGGTGGCAGAACGGGCATGGTGACGCCCCCGCGCTGAAGCC
GATCACCTGGAAGAACAAGCCGCTCGCCTCGGCAGCCCTGATGGCGGCGGTGCGTTATCAGCTCGTCCGGCTGGCCACGG
GGCGTGCCACCAGGCCGGGCATATCGCCACTCGTCGCTTTCCTGCTGGCCCAGTTGGCAGCGCGCCGGCAGGCGCGGGCG
TATCGCGCCTGGATGGCGTCCTCCCGGCCTGTTTCGCGCGTGGGCGCCAGAAAGCCTGAATGCCCTGCACCTGAGCGCAC
CGCGGCGGTTTTTGCCGGAAAGGAGTGA

Upstream 100 bases:

>100_bases
GCTCCGTCTCCCGAAGATGATCCTTGACCTTAAAGTTACCTTTAATGTCACGCTCTGACCAGAGTGTTCCTCACGGAGTC
GGCCTCATGGAGCTGGATTG

Downstream 100 bases:

>100_bases
GCGGAATGAACGATCCCGTCACAGAGGTGGAATTCGGTCCCTACACGGTGCCCGTCATCAAAGGCGGCTATTATGACCGG
TTTCGTTCGAACCCGGATCT

Product: flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSLFSPLTLPNGTVITNRIAKAAMEENMADADHAPSEGLIRLYRAWAEGGAGLIITGNVMVDARAMTGPAGVVLQDAQHL
DRFQDWATTARSGGGQIWMQINHPGRQMPASMAQETLAPSAIPLDLGRQSKMFPVPRAMRADDIADVTRRFIHTAGLAER
AGFDGVEIHAAHGYLLSQFLSPLSNRRTDQWGGSLENRARLLLDIVRGVRSSLRPDFAVSVKLNSADFQRGGFSPDDARA
VVRMLDGLGVDLVELSGGSYEAPAMMGSSRDERTLAREAYFLDFAKDIATVATMPLMVTGGIRRQDVAERVVADGIAMAG
MATALAIEPALPRRWQNGHGDAPALKPITWKNKPLASAALMAAVRYQLVRLATGRATRPGISPLVAFLLAQLAARRQARA
YRAWMASSRPVSRVGARKPECPAPERTAAVFAGKE

Sequences:

>Translated_435_residues
MSLFSPLTLPNGTVITNRIAKAAMEENMADADHAPSEGLIRLYRAWAEGGAGLIITGNVMVDARAMTGPAGVVLQDAQHL
DRFQDWATTARSGGGQIWMQINHPGRQMPASMAQETLAPSAIPLDLGRQSKMFPVPRAMRADDIADVTRRFIHTAGLAER
AGFDGVEIHAAHGYLLSQFLSPLSNRRTDQWGGSLENRARLLLDIVRGVRSSLRPDFAVSVKLNSADFQRGGFSPDDARA
VVRMLDGLGVDLVELSGGSYEAPAMMGSSRDERTLAREAYFLDFAKDIATVATMPLMVTGGIRRQDVAERVVADGIAMAG
MATALAIEPALPRRWQNGHGDAPALKPITWKNKPLASAALMAAVRYQLVRLATGRATRPGISPLVAFLLAQLAARRQARA
YRAWMASSRPVSRVGARKPECPAPERTAAVFAGKE
>Mature_434_residues
SLFSPLTLPNGTVITNRIAKAAMEENMADADHAPSEGLIRLYRAWAEGGAGLIITGNVMVDARAMTGPAGVVLQDAQHLD
RFQDWATTARSGGGQIWMQINHPGRQMPASMAQETLAPSAIPLDLGRQSKMFPVPRAMRADDIADVTRRFIHTAGLAERA
GFDGVEIHAAHGYLLSQFLSPLSNRRTDQWGGSLENRARLLLDIVRGVRSSLRPDFAVSVKLNSADFQRGGFSPDDARAV
VRMLDGLGVDLVELSGGSYEAPAMMGSSRDERTLAREAYFLDFAKDIATVATMPLMVTGGIRRQDVAERVVADGIAMAGM
ATALAIEPALPRRWQNGHGDAPALKPITWKNKPLASAALMAAVRYQLVRLATGRATRPGISPLVAFLLAQLAARRQARAY
RAWMASSRPVSRVGARKPECPAPERTAAVFAGKE

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=387, Percent_Identity=26.8733850129199, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787939, Length=219, Percent_Identity=33.7899543378995, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=348, Percent_Identity=35.0574712643678, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17540738, Length=357, Percent_Identity=34.733893557423, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17565138, Length=344, Percent_Identity=33.7209302325581, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17564188, Length=348, Percent_Identity=31.6091954022989, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI72001454, Length=354, Percent_Identity=32.4858757062147, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17559804, Length=348, Percent_Identity=31.3218390804598, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17566914, Length=350, Percent_Identity=34.8571428571429, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17566916, Length=358, Percent_Identity=29.3296089385475, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325086, Length=80, Percent_Identity=47.5, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321973, Length=80, Percent_Identity=46.25, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 46882; Mature: 46751

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFSPLTLPNGTVITNRIAKAAMEENMADADHAPSEGLIRLYRAWAEGGAGLIITGNVM
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEE
VDARAMTGPAGVVLQDAQHLDRFQDWATTARSGGGQIWMQINHPGRQMPASMAQETLAPS
EEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCC
AIPLDLGRQSKMFPVPRAMRADDIADVTRRFIHTAGLAERAGFDGVEIHAAHGYLLSQFL
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCHHHHCCCCCEEEEEHHHHHHHHHH
SPLSNRRTDQWGGSLENRARLLLDIVRGVRSSLRPDFAVSVKLNSADFQRGGFSPDDARA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHH
VVRMLDGLGVDLVELSGGSYEAPAMMGSSRDERTLAREAYFLDFAKDIATVATMPLMVTG
HHHHHHCCCCEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
GIRRQDVAERVVADGIAMAGMATALAIEPALPRRWQNGHGDAPALKPITWKNKPLASAAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHH
MAAVRYQLVRLATGRATRPGISPLVAFLLAQLAARRQARAYRAWMASSRPVSRVGARKPE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
CPAPERTAAVFAGKE
CCCCCCCEEEECCCC
>Mature Secondary Structure 
SLFSPLTLPNGTVITNRIAKAAMEENMADADHAPSEGLIRLYRAWAEGGAGLIITGNVM
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEE
VDARAMTGPAGVVLQDAQHLDRFQDWATTARSGGGQIWMQINHPGRQMPASMAQETLAPS
EEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCC
AIPLDLGRQSKMFPVPRAMRADDIADVTRRFIHTAGLAERAGFDGVEIHAAHGYLLSQFL
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCHHHHCCCCCEEEEEHHHHHHHHHH
SPLSNRRTDQWGGSLENRARLLLDIVRGVRSSLRPDFAVSVKLNSADFQRGGFSPDDARA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHH
VVRMLDGLGVDLVELSGGSYEAPAMMGSSRDERTLAREAYFLDFAKDIATVATMPLMVTG
HHHHHHCCCCEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
GIRRQDVAERVVADGIAMAGMATALAIEPALPRRWQNGHGDAPALKPITWKNKPLASAAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHH
MAAVRYQLVRLATGRATRPGISPLVAFLLAQLAARRQARAYRAWMASSRPVSRVGARKPE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
CPAPERTAAVFAGKE
CCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]