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The map label for this gene is cobQ [H]

Identifier: 162145873

GI number: 162145873

Start: 34793

End: 36271

Strand: Direct

Name: cobQ [H]

Synonym: GDI_0034

Alternate gene names: 162145873

Gene position: 34793-36271 (Clockwise)

Preceding gene: 162145872

Following gene: 162145874

Centisome position: 0.88

GC content: 71.53

Gene sequence:

>1479_bases
ATGGCCCGCGCCTTGATGTTCCAGGGAACGGGGTCGAGCGTGGGCAAGTCGGTGCTGGTCGCGGGCCTCGCGCGGGCGCT
GTCGCGGCGCGGTTTGCGGGTACGGCCCTTCAAGCCGCAGAACATGTCCAACAACGCCGCCGTCACCGCGGATGGCGGCG
AAATCGGGCGGGCACAGGCGTTGCAGGCGCTGGCATGCGGTATCGCGCCGTCGGTGCACATGAATCCCGTCCTGCTGAAA
CCGCAGAGTGCGACCGGGGCCCAGCTTGTCGTGGGCGGCAGGGCGCGCGGCACGGCCCGGGCGCGGGACTATCAGGCGAT
GAAGCCGGCCCTGATGCCCGAAATCCTGGCCAGCTTCGGGGCCCTGGCGCGCGAGGCCGACGTCGTCCTGGTCGAGGGCG
CGGGTTCGGCGTCCGAGGTCAATCTGCGCGCGGGCGACATCGCCAACATGGGCTTCGCGCAGGCGGCGGATGTCGATGTC
GTCCTGATCGGCGATATCGACCGGGGGGGCGTCATCGCCAGCCTGGTGGGCACGCAGGCCGTCCTGGACCCGGCGGATGC
GGACCGGATCAAGGGATTCATCGTCAATCGCATGCGCGGCGACCCATCCCTGTTCGCGACGGGCATGTCGGCGATCGCGG
CGCATACCGGCTGGCGGCCGCTGGGGCTGGTACCTTACCTGACGCGGGTCGAGGCCCTGCCGGCGGAGGACGCGGCGGAC
CTGGCTGCCGGGGGCGATGCGTCCGGCGCGCTTCGGATCGCCGTGCCGCACCTGCCCATGATCGCGAATTTCGACGATCT
CGACCCGCTGCGGGCCGAGGCCGCGCTGTCCGTCGGGATCGTGCCGCGCGGGCGGCCCCTGCCGGTATGCGACCTGATCA
TCCTGCCCGGATCGAAGGCGACGATTTCTGATCTATCGGTCCTGCGTCAGGAAGGATGGGATACCGACATCCGCGCGCAT
GTGCGGCGCGGCGGGCGGGTGCTGGGGATCTGCGGCGGATACCAGATGCTCGGCCGGACGATCGCCGACCCGGAGGGGGT
CGAGGGACCGCAGGGCGAGGTCGAGGCGCTCGGCCTGCTGGATGTGCGAACGGTGCTGACGCGGCGCAAGCGCCTGTCCG
AAGTCGCCGGCGTCCTGCTGCCGGAAGGGGCATCCCTGAGCGGCTACGAAATCCATGTCGGCCGGACCGATGGGCCGGAT
CGAACCCGGCCCTTCGCCCGCACCGGGTCCGGCCCGGATGGGGCGGTGTCGGCCGATGGCCGCGTGTGGGGGACCTATCT
GCACGGGATATTCCGGACGGGGGCCGCCCGGGCCGCGCTGCTGGCGCGACTGGGGACGGCGCGCATATCGCCGCGCGACC
ATGGCCACCTGGTGGATGACGCCCTGAACGCCCTGGCCGACCATCTGGAGGCGCATGTCGATATCGAGGCCCTGCTGGCC
CTGGCGCGGCCGGTCGCGGGTCTGTCCGGCGCGCCTTGA

Upstream 100 bases:

>100_bases
AGCTGGATCTGATCGCGGACGCCGACCTGGTCACGGAGATGACCCTGGTCAAACACCATTTCCGGGCCGGCGTGAAATCG
CAGGAGGGGATCGAATTCTG

Downstream 100 bases:

>100_bases
CCGGCACACCGGGCCAGGTCGATACCTCCCGGTCGATTTGACCCCGTCACGCATTCTGCTAGACGACGGAAAGGAGGAAA
TCGTTGAGCCGCAGAAACCA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MARALMFQGTGSSVGKSVLVAGLARALSRRGLRVRPFKPQNMSNNAAVTADGGEIGRAQALQALACGIAPSVHMNPVLLK
PQSATGAQLVVGGRARGTARARDYQAMKPALMPEILASFGALAREADVVLVEGAGSASEVNLRAGDIANMGFAQAADVDV
VLIGDIDRGGVIASLVGTQAVLDPADADRIKGFIVNRMRGDPSLFATGMSAIAAHTGWRPLGLVPYLTRVEALPAEDAAD
LAAGGDASGALRIAVPHLPMIANFDDLDPLRAEAALSVGIVPRGRPLPVCDLIILPGSKATISDLSVLRQEGWDTDIRAH
VRRGGRVLGICGGYQMLGRTIADPEGVEGPQGEVEALGLLDVRTVLTRRKRLSEVAGVLLPEGASLSGYEIHVGRTDGPD
RTRPFARTGSGPDGAVSADGRVWGTYLHGIFRTGAARAALLARLGTARISPRDHGHLVDDALNALADHLEAHVDIEALLA
LARPVAGLSGAP

Sequences:

>Translated_492_residues
MARALMFQGTGSSVGKSVLVAGLARALSRRGLRVRPFKPQNMSNNAAVTADGGEIGRAQALQALACGIAPSVHMNPVLLK
PQSATGAQLVVGGRARGTARARDYQAMKPALMPEILASFGALAREADVVLVEGAGSASEVNLRAGDIANMGFAQAADVDV
VLIGDIDRGGVIASLVGTQAVLDPADADRIKGFIVNRMRGDPSLFATGMSAIAAHTGWRPLGLVPYLTRVEALPAEDAAD
LAAGGDASGALRIAVPHLPMIANFDDLDPLRAEAALSVGIVPRGRPLPVCDLIILPGSKATISDLSVLRQEGWDTDIRAH
VRRGGRVLGICGGYQMLGRTIADPEGVEGPQGEVEALGLLDVRTVLTRRKRLSEVAGVLLPEGASLSGYEIHVGRTDGPD
RTRPFARTGSGPDGAVSADGRVWGTYLHGIFRTGAARAALLARLGTARISPRDHGHLVDDALNALADHLEAHVDIEALLA
LARPVAGLSGAP
>Mature_491_residues
ARALMFQGTGSSVGKSVLVAGLARALSRRGLRVRPFKPQNMSNNAAVTADGGEIGRAQALQALACGIAPSVHMNPVLLKP
QSATGAQLVVGGRARGTARARDYQAMKPALMPEILASFGALAREADVVLVEGAGSASEVNLRAGDIANMGFAQAADVDVV
LIGDIDRGGVIASLVGTQAVLDPADADRIKGFIVNRMRGDPSLFATGMSAIAAHTGWRPLGLVPYLTRVEALPAEDAADL
AAGGDASGALRIAVPHLPMIANFDDLDPLRAEAALSVGIVPRGRPLPVCDLIILPGSKATISDLSVLRQEGWDTDIRAHV
RRGGRVLGICGGYQMLGRTIADPEGVEGPQGEVEALGLLDVRTVLTRRKRLSEVAGVLLPEGASLSGYEIHVGRTDGPDR
TRPFARTGSGPDGAVSADGRVWGTYLHGIFRTGAARAALLARLGTARISPRDHGHLVDDALNALADHLEAHVDIEALLAL
ARPVAGLSGAP

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 50944; Mature: 50813

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARALMFQGTGSSVGKSVLVAGLARALSRRGLRVRPFKPQNMSNNAAVTADGGEIGRAQA
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCHHHH
LQALACGIAPSVHMNPVLLKPQSATGAQLVVGGRARGTARARDYQAMKPALMPEILASFG
HHHHHHCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHH
ALAREADVVLVEGAGSASEVNLRAGDIANMGFAQAADVDVVLIGDIDRGGVIASLVGTQA
HHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCHH
VLDPADADRIKGFIVNRMRGDPSLFATGMSAIAAHTGWRPLGLVPYLTRVEALPAEDAAD
HCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHH
LAAGGDASGALRIAVPHLPMIANFDDLDPLRAEAALSVGIVPRGRPLPVCDLIILPGSKA
HCCCCCCCCEEEEEECCCCEECCCCCCCCHHHHHHEEEECCCCCCCCCEEEEEEECCCCC
TISDLSVLRQEGWDTDIRAHVRRGGRVLGICGGYQMLGRTIADPEGVEGPQGEVEALGLL
HHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEEHH
DVRTVLTRRKRLSEVAGVLLPEGASLSGYEIHVGRTDGPDRTRPFARTGSGPDGAVSADG
HHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
RVWGTYLHGIFRTGAARAALLARLGTARISPRDHGHLVDDALNALADHLEAHVDIEALLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
LARPVAGLSGAP
HHHHHCCCCCCC
>Mature Secondary Structure 
ARALMFQGTGSSVGKSVLVAGLARALSRRGLRVRPFKPQNMSNNAAVTADGGEIGRAQA
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCHHHH
LQALACGIAPSVHMNPVLLKPQSATGAQLVVGGRARGTARARDYQAMKPALMPEILASFG
HHHHHHCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHHH
ALAREADVVLVEGAGSASEVNLRAGDIANMGFAQAADVDVVLIGDIDRGGVIASLVGTQA
HHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCHH
VLDPADADRIKGFIVNRMRGDPSLFATGMSAIAAHTGWRPLGLVPYLTRVEALPAEDAAD
HCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHH
LAAGGDASGALRIAVPHLPMIANFDDLDPLRAEAALSVGIVPRGRPLPVCDLIILPGSKA
HCCCCCCCCEEEEEECCCCEECCCCCCCCHHHHHHEEEECCCCCCCCCEEEEEEECCCCC
TISDLSVLRQEGWDTDIRAHVRRGGRVLGICGGYQMLGRTIADPEGVEGPQGEVEALGLL
HHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEEHH
DVRTVLTRRKRLSEVAGVLLPEGASLSGYEIHVGRTDGPDRTRPFARTGSGPDGAVSADG
HHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
RVWGTYLHGIFRTGAARAALLARLGTARISPRDHGHLVDDALNALADHLEAHVDIEALLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
LARPVAGLSGAP
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA