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The map label for this gene is yaeI [H]

Identifier: 161984987

GI number: 161984987

Start: 170274

End: 171065

Strand: Reverse

Name: yaeI [H]

Synonym: SBO_0153

Alternate gene names: 161984987

Gene position: 171065-170274 (Counterclockwise)

Preceding gene: 82542765

Following gene: 82542763

Centisome position: 3.78

GC content: 51.39

Gene sequence:

>792_bases
TTGCAGGCTACTGCCGCGACGATAGCCACGAGCTCAGGTTTCGGTTATATGCATTACTGGGAGCCAGGCTGGTTTGAATT
AATCCGTCACCGCCTCGCCTTTTTTAAAGACAACGCAGCACCATTCAAAATTCTTTTTCTGGCCGATCTCCATTACTCTC
GTTTTGTTCCTTTAAGCCTGATTTCTGACGCGATTGCTCTTGGCATAGAACAAAAGCCCGATTTGATATTACTGGGCGGC
GATTACGTATTATTTGATATGCCGCTGAATTTTTCGGCGTTTAGTGATGTCCTCTCCCCCCTTGCCGAATGTGCGCCGAC
GTTTGCCTGCTTCGGCAACCACGATCGCCCTGTTGGTACAGAAAAAAATCACTTAATTGGCGAGACGTTGAAATCAGTGG
GCATCACGGTGTTGTTTAACCAGGCTACGGTGATCGCCACGCCGAACAGGCAATTCGAACTGGTAGGCACCGGTGACTTA
TGGGCCGGACAATGCAAACCACCTCCCGCCAGCGAAGCAAATCTGCCAAGACTGATGCTGGCACATAATCCCGACAGCAA
AGAAGTCATGCGTGATGAACCCTGGGATCTGATGCTGTGCGGCCATACTCACGGCGGGCAACTGCGCGTACCGTTGGTTG
GTGAACCTTTTGCCCCTGTCGAAGATAAACGTTACGTCGCCGGATTAAATGCCTTTGGCGAACGACACATCTACACAACC
CGTGGCGTGGGCAGTTTGTATGGTTTGCGTCTGAACTGCCGCCCGGAAGTAACGATGCTGGAACTGGTGTAG

Upstream 100 bases:

>100_bases
CGAACTGCTGCGTACCATCGACTAAAAGTATGCACACGGGCAGCACGACGCTGCCCGATTTTTTTTGCAGGGATGAGCGA
TGATTTCACGCCGCCGATTG

Downstream 100 bases:

>100_bases
AACGTGAATTTTATCACCACAATAAAGCAGGTAATTTTTTGATTTTTACGCCATGATAGAAGTCTGGAAGAAAAAGGCTT
TCTGCGATCACAGTCGAGCC

Product: phosphodiesterase YaeI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKDNAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGG
DYVLFDMPLNFSAFSDVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFELVGTGDL
WAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVPLVGEPFAPVEDKRYVAGLNAFGERHIYTT
RGVGSLYGLRLNCRPEVTMLELV

Sequences:

>Translated_263_residues
MQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKDNAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGG
DYVLFDMPLNFSAFSDVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFELVGTGDL
WAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVPLVGEPFAPVEDKRYVAGLNAFGERHIYTT
RGVGSLYGLRLNCRPEVTMLELV
>Mature_263_residues
MQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKDNAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGG
DYVLFDMPLNFSAFSDVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFELVGTGDL
WAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVPLVGEPFAPVEDKRYVAGLNAFGERHIYTT
RGVGSLYGLRLNCRPEVTMLELV

Specific function: Shows phosphodiesterase activity, hydrolyzing phosphodiester bond in the artificial chromogenic substrate bis-p- nitrophenyl phosphate (bis-pNPP) [H]

COG id: COG1408

COG function: function code R; Predicted phosphohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081695, Length=263, Percent_Identity=98.0988593155894, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 29088; Mature: 29088

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKDNAAPFKILFLADLHYSRFVPLSL
CCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHCCHHHH
ISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFSDVLSPLAECAPTFACFGNHDRPVGT
HHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
EKNHLIGETLKSVGITVLFNQATVIATPNRQFELVGTGDLWAGQCKPPPASEANLPRLML
CCCHHHHHHHHHCCEEEEEECEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
AHNPDSKEVMRDEPWDLMLCGHTHGGQLRVPLVGEPFAPVEDKRYVAGLNAFGERHIYTT
EECCCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEEE
RGVGSLYGLRLNCRPEVTMLELV
CCCCCEEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKDNAAPFKILFLADLHYSRFVPLSL
CCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHCCHHHH
ISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFSDVLSPLAECAPTFACFGNHDRPVGT
HHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
EKNHLIGETLKSVGITVLFNQATVIATPNRQFELVGTGDLWAGQCKPPPASEANLPRLML
CCCHHHHHHHHHCCEEEEEECEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
AHNPDSKEVMRDEPWDLMLCGHTHGGQLRVPLVGEPFAPVEDKRYVAGLNAFGERHIYTT
EECCCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEEE
RGVGSLYGLRLNCRPEVTMLELV
CCCCCEEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]