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The map label for this gene is ygaA [H]

Identifier: 161984874

GI number: 161984874

Start: 2819557

End: 2821071

Strand: Direct

Name: ygaA [H]

Synonym: SBO_2809

Alternate gene names: 161984874

Gene position: 2819557-2821071 (Clockwise)

Preceding gene: 82545213

Following gene: 82545223

Centisome position: 62.38

GC content: 56.96

Gene sequence:

>1515_bases
ATGAGTTTTTCCGTTGATGTGCTGGCGAATATCGCCATCGAATTGCAGCGTGGGATTGGTCATCAGGATCGTTTTCAGCG
CCTGATCACCACGCTACGTCAGGTGCTGGAGTGCGATGCGTCTGCGTTGCTACGTTACGATTCGCGGCAGTTTATTCCGC
TTGCCATCGACGGTCTGGCAAAGGGTGTACTCGGTAGACGCTTTGCGCTGGAAGGGCATCCACGGCTGGAAGCGATTGCC
CGCGCCGGGGATGTGGTGCGCTTTCCCGCAGACAGCGAATTGCCCGATCCCTATGACGGTTTGATTCCTGGGCAGGAGAG
TCTGAAGGTTCACGCCTGCGTTGGTCTGCCATTGTTTGCCGGGCAAAACCTGATCGGCGCACTGACGCTCGACGGGATGC
AGCCCGATCAGTTCGATGTTTTCAGCGACGAAGAGCTACGGCTGATTGCTGCGCTGGCGGCGGGAGCGTTAAGCAATGCG
TTGCTGATTGAACAACTGGAAAGCCAGAATATGATGCCAGGCGATGCCACGCCGTTTGAAGCGGTGAAACAGACGCAGAT
GATTGGCTTGTCCCCTGGCATGACGCAACTGAAAAAAGAGATTGAGATTGTGGCGGCGTCCGATCTCAACGTCCTGATCA
GCGGTGAGACGGGAACCGGTAAGGAGCTGGTGGCGAAAGCGATTCATGAAGCCTCGCCACGGGCGGTGAATCCGCTGGTC
TATCTCAACTGTGCTGCACTGCCGGAAAGTGTGGCGGAAAGTGAGTTGTTCGGGCATGTGAAAGGAGCGTTTACTGGCGC
TATCAGTAACCGCAGCGGGAAGTTTGAAATGGCGGATAACGGCACTCTGTTTCTGGATGAGATCGGCGAGTTGTCGTTGG
CATTGCAGGCCAAGCTGCTGAGGGTGTTGCAGTATGGCGATATTCAGCGCGTTGGCGATGACCGTAGTTTGCGGGTCGAT
GTGCGCGTGCTGGCGGCGACTAACCGCGACTTACGCGAAGAGGTGCTGGCAGGGCGATTTCGCGCTGACTTGTTTCATCG
CCTGAGCGTGTTTCCACTTTCGGTGCCGCCGCTGCGTGAGCGGGGCGATGATGTCATTCTGCTGGCGGGGTATTTCTGCG
AACAGTGTCGTTTGCGGCTGGGGCTCTCCCGCGTGGTATTAAGTGCCGGAGCGCGAAATTTACTGCAACACTATCGTTTT
CCGGGGAACGTGCGCGAACTGGAACATGCTATTCATCGGGCGGTAGTGCTGGCGAGAGCCACCCGCAACGGCGATGAAGT
GATTCTTGAGGCGCAACATTTTGCTTTTCCTGAGGTGACGTTGCCGCCGCCAGAAGCGGCGGCGGTGCCCGTTGTTAAGC
AAAACCTGCGTGAAGCGACAGAAGCGTTCCAGCGTGAAACTATTCGCCAGGCACTGGCACAAAATCATCATAACTGGGCT
GCCTGCGCGCGGATGCTGGAAACCGACGTCGCCAACCTGCATCGGCTGGCGAAACGTCTGGGAATGAAGGATTAA

Upstream 100 bases:

>100_bases
TCTATAAATAAAATTATGCTCATTAATGAGTAGTCAAAATGACTATCAATCAAATGGTCGATATGACAATATCTATAGTC
AAATTGACAGTGAGGCAAAG

Downstream 100 bases:

>100_bases
ATAATCCCGGCCTGATAGAAATCCTGCAGGTTAATTGCGCCGGTGAGTTTGCCGTTTTCATCCACCACCGGTGCGGCAGT
GATTTTGCGCTTCATCAGGA

Product: anaerobic nitric oxide reductase transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIA
RAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGARNLLQHYRF
PGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
ACARMLETDVANLHRLAKRLGMKD

Sequences:

>Translated_504_residues
MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIA
RAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVD
VRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGARNLLQHYRF
PGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
ACARMLETDVANLHRLAKRLGMKD
>Mature_503_residues
SFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIAR
AGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNAL
LIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLVY
LNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDV
RVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGARNLLQHYRFP
GNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWAA
CARMLETDVANLHRLAKRLGMKD

Specific function: Required for the expression of anaerobic nitric oxide (NO) reductase, acts as a transcriptional activator for at least the norVW operon. Activation also requires sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082152, Length=504, Percent_Identity=98.2142857142857, Blast_Score=997, Evalue=0.0,
Organism=Escherichia coli, GI1789087, Length=363, Percent_Identity=41.0468319559229, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI87082117, Length=316, Percent_Identity=47.1518987341772, Blast_Score=255, Evalue=6e-69,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.9648562300319, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1788550, Length=299, Percent_Identity=45.819397993311, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1790299, Length=336, Percent_Identity=41.0714285714286, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788905, Length=316, Percent_Identity=39.5569620253165, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1789233, Length=330, Percent_Identity=39.0909090909091, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=39.1437308868502, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=338, Percent_Identity=37.8698224852071, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786524, Length=243, Percent_Identity=46.5020576131687, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=36.9294605809129, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081858, Length=220, Percent_Identity=34.0909090909091, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55248; Mature: 55117

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEHHHHHHHH
KGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFA
HHHHHCEEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHC
GQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNALLIEQLESQNMMPGDATPFE
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
HHHHHHHCCCCCCHHHHHHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RGDDVILLAGYFCEQCRLRLGLSRVVLSAGARNLLQHYRFPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
TRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
CCCCCEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ACARMLETDVANLHRLAKRLGMKD
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALLRYDSRQFIPLAIDGLA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEHHHHHHHH
KGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFA
HHHHHCEEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHC
GQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNALLIEQLESQNMMPGDATPFE
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLV
HHHHHHHCCCCCCHHHHHHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEE
YLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLL
EEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHH
RVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRE
HHHHCCCHHHCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RGDDVILLAGYFCEQCRLRLGLSRVVLSAGARNLLQHYRFPGNVRELEHAIHRAVVLARA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
TRNGDEVILEAQHFAFPEVTLPPPEAAAVPVVKQNLREATEAFQRETIRQALAQNHHNWA
CCCCCEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ACARMLETDVANLHRLAKRLGMKD
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA