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The map label for this gene is tuf1 [H]

Identifier: 161936276

GI number: 161936276

Start: 188831

End: 190015

Strand: Direct

Name: tuf1 [H]

Synonym: Sputw3181_0154

Alternate gene names: 161936276

Gene position: 188831-190015 (Clockwise)

Preceding gene: 120596986

Following gene: 120596988

Centisome position: 4.01

GC content: 48.35

Gene sequence:

>1185_bases
GTGGCAAAAGCTAAATTTGAACGTATTAAGCCTCACGTAAACGTGGGCACCATTGGTCACGTTGACCATGGTAAAACCAC
TCTGACTGCAGCTATCTCTCACGTACTGGCTAAGACCTACGGTGGCGAAGCTAAAGACTTCTCTCAAATCGATAACGCTC
CAGAAGAGCGTGAGCGCGGTATTACCATCAATACCTCTCACATCGAATATGACACGCCATCACGCCACTACGCCCACGTA
GACTGCCCAGGCCACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCACAGATGGACGGCGCGATTCTGGTAGTCGC
TTCAACAGACGGTCCAATGCCACAGACTCGTGAGCACATCCTGCTTTCTCGTCAGGTTGGCGTACCATTCATCATCGTAT
TCATGAACAAATGTGACATGGTAGATGACGAAGAGCTGTTAGAGCTAGTTGAGATGGAAGTGCGTGAACTGTTATCAGAA
TACGATTTCCCAGGTGATGACTTACCGGTAATCCAAGGTTCAGCTCTGAAAGCGCTAGAAGGCGAGCCAGAGTGGGAAGC
AAAAATCCTTGAATTAGCAGCGGCGCTGGATTCTTACATTCCAGAACCACAACGTGACATCGATAAGCCGTTCCTACTGC
CAATCGAAGACGTATTCTCAATTTCAGGCCGTGGTACAGTAGTAACAGGTCGTGTTGAGCGTGGTATTGTACGCGTAGGC
GACGAAGTTGAAATCGTTGGTGTACGTGCGACAACTAAGACAACGTGTACTGGTGTAGAAATGTTCCGTAAACTGCTTGA
CGAAGGTCGTGCAGGTGAGAACTGTGGTATTTTGTTACGTGGTACTAAGCGTGATGACGTAGAACGTGGTCAAGTATTAG
CGAAGCCAGGTTCAATCAACCCACACACCACTTTTGAATCAGAAGTTTACGTACTGTCAAAAGAAGAAGGTGGCCGTCAC
ACGCCATTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACAACTGACGTAACCGGTACTATCGAACTGCCAGAAGG
CGTAGAGATGGTAATGCCAGGCGACAACATCAAGATGGTAGTGACACTGATTTGCCCAATCGCGATGGACGAGGGTTTAC
GCTTCGCAATCCGTGAAGGCGGTCGTACAGTGGGTGCAGGTGTAGTAGCTAAAATCATTGCTTAA

Upstream 100 bases:

>100_bases
GAAAGCGATTATTGAATCTCGTAGCTAATTTCCAGTTACGACATAAATGTTATTATGGTCCAGCTTTGACTGGACTATTC
TGAAAAGAAAGGAATATATC

Downstream 100 bases:

>100_bases
TAGCGATAGAGATTAGTGTGAAAAGGAAGCTTCGGCTTCCTTTTTTGTTTAAGGGCTAGTTTTCTCCATAAATTTGTGTA
AAATGCTCGCCACTCTGACG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKILELAAALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVRVG
DEVEIVGVRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLAKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_394_residues
MAKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKILELAAALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVRVG
DEVEIVGVRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLAKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
>Mature_393_residues
AKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPSRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEY
DFPGDDLPVIQGSALKALEGEPEWEAKILELAAALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVRVGD
EVEIVGVRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLAKPGSINPHTTFESEVYVLSKEEGGRHT
PFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.9620253164557, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=354, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=29.5612009237875, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=29.5612009237875, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=29.082774049217, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=29.5652173913043, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018522, Length=344, Percent_Identity=29.6511627906977, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI194018520, Length=344, Percent_Identity=29.6511627906977, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI194097354, Length=344, Percent_Identity=29.6511627906977, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI46094014, Length=345, Percent_Identity=30.1449275362319, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4503507, Length=364, Percent_Identity=28.021978021978, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI53729339, Length=231, Percent_Identity=30.7359307359307, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=231, Percent_Identity=30.7359307359307, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=263, Percent_Identity=25.0950570342205, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=86.8020304568528, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=86.8020304568528, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=29.4429708222812, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=30.1587301587302, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789108, Length=311, Percent_Identity=28.6173633440514, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.8771929824561, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=45.7142857142857, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=447, Percent_Identity=29.7539149888143, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=447, Percent_Identity=29.7539149888143, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=28.4064665127021, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=28.5057471264368, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=28.4722222222222, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=237, Percent_Identity=32.4894514767932, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=339, Percent_Identity=27.7286135693215, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17509919, Length=447, Percent_Identity=26.8456375838926, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=152, Percent_Identity=36.1842105263158, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.4760705289673, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.6306306306306, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.6306306306306, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=460, Percent_Identity=23.9130434782609, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=291, Percent_Identity=27.8350515463918, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=252, Percent_Identity=30.5555555555556, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=57.2519083969466, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=57.2519083969466, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=383, Percent_Identity=51.6971279373368, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=28.8590604026846, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=28.8590604026846, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=28.8590604026846, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=28.8590604026846, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.9532293986637, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.9532293986637, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.4701986754967, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.5229357798165, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17137380, Length=335, Percent_Identity=30.7462686567164, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647046, Length=417, Percent_Identity=26.1390887290168, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647048, Length=417, Percent_Identity=26.1390887290168, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28572034, Length=163, Percent_Identity=36.8098159509202, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43290; Mature: 43159

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCCHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKILELAAALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGVRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLAKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
EEEHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKILELAAALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVRVG
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGVRATTKTTCTGVEMFRKLLDEGRAGENCGILLRGTKRDDVERGQVLAKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
EEEHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA