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The map label for this gene is purD [H]

Identifier: 161871014

GI number: 161871014

Start: 2142172

End: 2143443

Strand: Reverse

Name: purD [H]

Synonym: NMCC_2110

Alternate gene names: 161871014

Gene position: 2143443-2142172 (Counterclockwise)

Preceding gene: 161871016

Following gene: 161871013

Centisome position: 99.54

GC content: 50.31

Gene sequence:

>1272_bases
ATGAAACTGCTGGTTATCGGTAATGGCGGTCGCGAACACGCGCTGGCTTGGAAATTGGCGCAGTCGCCTAAAGTGGAAAC
AGTATTTGTTGCGCCTGGTAATGCCGGTACAGCGATTGAACCCAAGTTGCAAAACATCGACTTGACTGCGCATCAGGATT
TGATTGAATTCAGTCGTAAAGAAAATATTGTTTTTACCGTCGTCGGCCCTGAAGCGCCTTTGGCGGCAGGTGTTGTGGAT
GATTTCCGTGCCGCAGGACTAAAAATATTCGGTCCGACACAATATGCGGCGCAGTTGGAAAGTTCCAAAGATTTCGCCAA
AGCATTTATGGCGAAATACAACATTCCGACCGCGCAATATCAAACCTTTGAAAACGCCGATGCCGCACATGATTACGTCA
ATCAGAAAGGTGCGCCTATCGTTATCAAAGCCGATGGTTTGGCGGCAGGTAAAGGCGTGATTGTGGCGATGACTTTGGAT
GAAGCGCATGCTGCGATTGATGATATGCTGCTGGACAACAAAATGGGCAATGCCGGCGCGCGTGTTGTGATTGAAGATTT
CCTGCAAGGCGAAGAAGCGAGTTTTATCGTCATGGTTGATGGCAATAATGTACTGCCTATGGCAACCAGCCAAGACCACA
AGCGTCTTTTAGACGGCGACAAAGGTCTGAATACGGGCGGTATGGGTGCGTACAGCCCTGCGCCTGTGGTAACGCCTGTT
GTGTACGAGCGCGCGATGAATGAAATTATTTTGCCGACCGTAGCAGGGATGAAAGCGGAGGGGCATGAGTTCACCGGTTT
CCTGTACGCAGGTTTGATGATTGATCAAAGCGGTGCACCCTATACGATTGAGTTTAACTGCCGTTTCGGCGATCCTGAAA
CCCAGCCGATTATGAGCCGACTGAACAGCGACTTATCGGATTTGGTTGAAGCGGCAATAGACGGCAGACTCGATAGCGTA
ACCGCGGAATGGAGCCCGCAAACTGCCGTGGGCGTGGTACTGGCGGCGCAAAATTACCCCGAAACGCCCAAAAAAGGCGA
TGTTATTTCCGGTCTGGATGCTGCCAACCAAGTAGGCAAAGTTTTCCATGCAGGTACAACGGCAAACGAGAAAGGTGACG
TATTGACCAACGGCGGACGCGTATTGTGTGTTGTGGGATTGGGCGACAATGTGGCGCAAGCGAAAGCTAAGGCATATGGC
GCGTTGGAAAAAATCAGTTTCGACGGGATGCAATACCGTAAAGACATTGCCGATAAAGCGATTAACCGTTAA

Upstream 100 bases:

>100_bases
AGCGGTCTTTTGTTTGATTCTACTAAAATTTCTTCAGATGGGATAAAAGTCCCTTTGTATCGAGTTTTCGTTAAAAGTAC
ATATTTGTCAAGGAGATGGT

Downstream 100 bases:

>100_bases
CTAGAAAGAGGATGATGCCGTCTGAAGCCAAGTATGACTGATGTGCTTCAGACGGTATTTTATATTATGAGACCTTTGCA
AAATTCCCCAAAATCCCCTA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQNIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVD
DFRAAGLKIFGPTQYAAQLESSKDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLDGDKGLNTGGMGAYSPAPVVTPV
VYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQSGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSV
TAEWSPQTAVGVVLAAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVAQAKAKAYG
ALEKISFDGMQYRKDIADKAINR

Sequences:

>Translated_423_residues
MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQNIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVD
DFRAAGLKIFGPTQYAAQLESSKDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLDGDKGLNTGGMGAYSPAPVVTPV
VYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQSGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSV
TAEWSPQTAVGVVLAAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVAQAKAKAYG
ALEKISFDGMQYRKDIADKAINR
>Mature_423_residues
MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQNIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVD
DFRAAGLKIFGPTQYAAQLESSKDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLDGDKGLNTGGMGAYSPAPVVTPV
VYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQSGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSV
TAEWSPQTAVGVVLAAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVAQAKAKAYG
ALEKISFDGMQYRKDIADKAINR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=48.7058823529412, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=48.7058823529412, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=48.7058823529412, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=48.7058823529412, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=64.3192488262911, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=43.1603773584906, Blast_Score=316, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=44.0909090909091, Blast_Score=338, Evalue=9e-94,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.707656612529, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=45.707656612529, Blast_Score=352, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44945; Mature: 44945

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQNIDLTAHQDLIEFSRK
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHCC
ENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESSKDFAKAFMAKYNIPTAQY
CCEEEEEECCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHH
QTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLDEAHAAIDDMLLDNKMGNAGA
HCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCC
RVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLDGDKGLNTGGMGAYSPAPVVTPV
HHHHHHHHCCCCCEEEEEECCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
VYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQSGAPYTIEFNCRFGDPETQPIMSR
HHHHHHHHEECHHHCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEEEECCCCCHHHHHHH
LNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVLAAQNYPETPKKGDVISGLDAANQVGK
HCCCHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
VFHAGTTANEKGDVLTNGGRVLCVVGLGDNVAQAKAKAYGALEKISFDGMQYRKDIADKA
HEECCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
INR
CCC
>Mature Secondary Structure
MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQNIDLTAHQDLIEFSRK
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHCC
ENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESSKDFAKAFMAKYNIPTAQY
CCEEEEEECCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHH
QTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLDEAHAAIDDMLLDNKMGNAGA
HCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCC
RVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLDGDKGLNTGGMGAYSPAPVVTPV
HHHHHHHHCCCCCEEEEEECCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
VYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQSGAPYTIEFNCRFGDPETQPIMSR
HHHHHHHHEECHHHCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEEEECCCCCHHHHHHH
LNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVLAAQNYPETPKKGDVISGLDAANQVGK
HCCCHHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
VFHAGTTANEKGDVLTNGGRVLCVVGLGDNVAQAKAKAYGALEKISFDGMQYRKDIADKA
HEECCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
INR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]