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The map label for this gene is prfB [H]

Identifier: 161871001

GI number: 161871001

Start: 2132256

End: 2133314

Strand: Reverse

Name: prfB [H]

Synonym: NMCC_2097

Alternate gene names: 161871001

Gene position: 2133314-2132256 (Counterclockwise)

Preceding gene: 161871002

Following gene: 161870998

Centisome position: 99.07

GC content: 52.69

Gene sequence:

>1059_bases
TTGGAAAAGCGCAGCGAAGACATCCGAGTTTATATGGATTATCAGGGTAAGAAAGACCGATTGGAAGAAGTTATCGGTCT
TTCCGAAGACCCCGAACTTTGGAACGACCCGAAACGCGCCCAAGAAATCGGCAAAGAGCGCAAAATCCTCGAAGGCATCG
TGTTGACGCTCGACAACATCGCTTCGGGCATCGAAGACAACCGCATGCTGATTGAAATGACCGTCGAAGAAAACGACGAA
GAAGGTTTTGCCGCCGTGCAGGAAGATGTGGCGGGGCTGGAAAAACAGATGGCGGATTTGGAGTTCAAACGGATGTTCAA
CCAGCCCGCCGACCCGAACAACTGCTTTATCGACATCACCGCAGGCGCGGGCGGTACGGAAGCGGAAGACTGGGCGGGTA
TGCTGTTCCGTATGTACAGCCGCTACGCCGAGCGCAAAGGCTTCAAAATCGAAATCCTCGAAGAAGACGACGGCGAAATC
GCGGGCATCAACCGCGCGACTATCCGAGTGGAAGGCGAATACGCCTATGGTTTGCTGCGTACCGAAACCGGCGTTCACCG
CTTGGTGCGCTATTCACCGTTTGACTCGAACAACAAACGCCATACTTCGTTTGCCTCCGTGTTCGTTTACCCTGAAATCG
ATGATTCCATTGAAATCGAAATCAACCCCGCCGATTTGCGTATCGACACCTATCGCGCATCGGGCGCGGGCGGTCAGCAC
ATCAATAAAACCGACTCCGCCGTGCGCATTACCCACGAGCCGACGGGGATTGTGGTGCAATGTCAAAACGACCGTTCGCA
ACACGCCAACAAAGCCGCCGCGATGGAAATGTTGAAGTCCAAACTGTATGAATTGGAAATGCGCAAACGCAATGAAGAGA
AACAGGCGTTGGAAGAAGGTAAATCCGATGTAGGTTGGGGTAGCCAAATCCGTTCGTATGTTTTGGATTCCTCACGTATC
AAAGACTTGCGTACAGGCTACGAAGTCGGCAACACCAAAGCCGTATTGGATGGCGATTTGGACGGCTTTATCGAAGCCAG
CCTGAAACAGGGCGTGTAG

Upstream 100 bases:

>100_bases
AAATACCCGAATCCCGTACAATACGCCCCTAAAATTTTGAAAGAACAAAAGAATCATGGAAGCCGAAGTAATCAACCAGC
TCAACAATACCCTGAATGAT

Downstream 100 bases:

>100_bases
TTTGAACCGTATGCCGTCTGAAACCTGTACCGTTTCAGACGGCATTTTTGCGTTTCGTGTTATAATCCGCGCCGCACACG
GGACGAGCAGACAGTCGCCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MEKRSEDIRVYMDYQGKKDRLEEVIGLSEDPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDE
EGFAAVQEDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAERKGFKIEILEEDDGEI
AGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTSFASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQH
INKTDSAVRITHEPTGIVVQCQNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSRI
KDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV

Sequences:

>Translated_352_residues
MEKRSEDIRVYMDYQGKKDRLEEVIGLSEDPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDE
EGFAAVQEDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAERKGFKIEILEEDDGEI
AGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTSFASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQH
INKTDSAVRITHEPTGIVVQCQNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSRI
KDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV
>Mature_352_residues
MEKRSEDIRVYMDYQGKKDRLEEVIGLSEDPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDE
EGFAAVQEDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAERKGFKIEILEEDDGEI
AGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTSFASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQH
INKTDSAVRITHEPTGIVVQCQNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSRI
KDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=274, Percent_Identity=36.8613138686131, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI34577120, Length=281, Percent_Identity=35.9430604982206, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI166795305, Length=170, Percent_Identity=40.5882352941176, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI2367172, Length=352, Percent_Identity=64.4886363636364, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1787462, Length=342, Percent_Identity=36.5497076023392, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=287, Percent_Identity=32.0557491289199, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321295, Length=269, Percent_Identity=36.4312267657993, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19921226, Length=305, Percent_Identity=36.3934426229508, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39780; Mature: 39780

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRSEDIRVYMDYQGKKDRLEEVIGLSEDPELWNDPKRAQEIGKERKILEGIVLTLDNI
CCCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASGIEDNRMLIEMTVEENDEEGFAAVQEDVAGLEKQMADLEFKRMFNQPADPNNCFIDIT
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
AGAGGTEAEDWAGMLFRMYSRYAERKGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCEEEEEEECCEEEEEEE
TETGVHRLVRYSPFDSNNKRHTSFASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQH
CHHHHHHHHEECCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCEEEEEEECCCCCCCC
INKTDSAVRITHEPTGIVVQCQNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEG
CCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
KSDVGWGSQIRSYVLDSSRIKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV
CCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MEKRSEDIRVYMDYQGKKDRLEEVIGLSEDPELWNDPKRAQEIGKERKILEGIVLTLDNI
CCCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASGIEDNRMLIEMTVEENDEEGFAAVQEDVAGLEKQMADLEFKRMFNQPADPNNCFIDIT
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
AGAGGTEAEDWAGMLFRMYSRYAERKGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCEEEEEEECCEEEEEEE
TETGVHRLVRYSPFDSNNKRHTSFASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQH
CHHHHHHHHEECCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCEEEEEEECCCCCCCC
INKTDSAVRITHEPTGIVVQCQNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEG
CCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
KSDVGWGSQIRSYVLDSSRIKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV
CCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA