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The map label for this gene is xthA [H]

Identifier: 161870674

GI number: 161870674

Start: 1794929

End: 1795699

Strand: Reverse

Name: xthA [H]

Synonym: NMCC_1746

Alternate gene names: 161870674

Gene position: 1795699-1794929 (Counterclockwise)

Preceding gene: 161870675

Following gene: 161870673

Centisome position: 83.39

GC content: 56.68

Gene sequence:

>771_bases
ATGAAAATCACCACTTGGAACGTCAATTCGCTCAATGTGCGGCTGCCGCAGGTGCAAAACCTGCTTGCCGACAATCCGCC
CGATATTTTGGTTTTGCAGGAACTCAAACTCGATCAGGACAAATTTCCGGCCGCCGCTTTGCAAATGATGGGCTGGCACT
GTGTTTGGAGCGGGCAGAAAACCTACAACGGCGTGGCAATCGTCAGCCGCAACGCGCCGGAAGACGTGCATATCGGGCTG
CCCGCGCTGCCGGACGATCCGCAACGGCGCGTGATTGCGGCAACCGTCGGCGGTGTGCGCGTCATCAATGTCTATTGCGT
CAACGGCGAAGCCCTTGACAGCCCGAAATTCAAATATAAAGAACAATGGTTTGCCGCACTGACGGAGTTTGTCCGCGATG
AAATGACCCGCTACGGCAAACTGGTGCTACTGGGCGATTTCAATATCGCGCCTGCCGATGCGGACTGTTACGACCCTGAA
AAATGGCACGAAAAAATCCACTGTTCGTCCGTCGAACGGCAGTGGTTTCAAAACCTGCTGGATTTGGGACTGACCGACAG
CCTGCGCCAAGTCCATCCCGAAGGCGCGTTTTACACATGGTTCGACTATCGCGGCGCGATGTTCCAACGCAAACTGGGCC
TGCGTATCGACCATATTTTGGTGTCGCCTGCGATGGCGGCGGCGTTGAAGGATGTCCGCGTCGATTTGGAGACGCGCGCG
CTGGAGCGTCCGAGCGACCACGCGCCGGTGACGGCAGAATTCGATTGGTAA

Upstream 100 bases:

>100_bases
TGCGGACAATCCGCGATTTGGAAAACAAACGCGCGGTATGGTGTTAAAATCCTTTCCTTTTGCCGTCTGAACGTTTCAGA
CGGCATTTTTCGGAAATGTT

Downstream 100 bases:

>100_bases
AAGACCGTGTTTTGATATGGCGTTGACAAACATCCATATCTTTAAGTGATTCAATAGGATAGGCTGCTGACTAACCGAAA
ATAATTGCCTTTTCCCTGAC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPAAALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGL
PALPDDPQRRVIAATVGGVRVINVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDHILVSPAMAAALKDVRVDLETRA
LERPSDHAPVTAEFDW

Sequences:

>Translated_256_residues
MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPAAALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGL
PALPDDPQRRVIAATVGGVRVINVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDHILVSPAMAAALKDVRVDLETRA
LERPSDHAPVTAEFDW
>Mature_256_residues
MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPAAALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGL
PALPDDPQRRVIAATVGGVRVINVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDHILVSPAMAAALKDVRVDLETRA
LERPSDHAPVTAEFDW

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=259, Percent_Identity=31.2741312741313, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI18375503, Length=259, Percent_Identity=31.2741312741313, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI18375501, Length=259, Percent_Identity=31.2741312741313, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=36.0902255639098, Blast_Score=178, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=29.6577946768061, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221330655, Length=262, Percent_Identity=29.7709923664122, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136678, Length=262, Percent_Identity=29.7709923664122, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29118; Mature: 29118

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPAAALQMMGWHCVWSGQK
CEEEEECCCEEEEECHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHCCEEEECCCC
TYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVINVYCVNGEALDSPKFKYK
CCCCEEEEECCCCCCEEECCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCHHHH
EQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPEKWHEKIHCSSVERQWFQNLL
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDHILVSPAMAAALKDVRVDLETRA
HCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCHHHHH
LERPSDHAPVTAEFDW
HCCCCCCCCEEEECCC
>Mature Secondary Structure
MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPAAALQMMGWHCVWSGQK
CEEEEECCCEEEEECHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHCCEEEECCCC
TYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVINVYCVNGEALDSPKFKYK
CCCCEEEEECCCCCCEEECCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCHHHH
EQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPEKWHEKIHCSSVERQWFQNLL
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDHILVSPAMAAALKDVRVDLETRA
HCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCHHHHH
LERPSDHAPVTAEFDW
HCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]