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The map label for this gene is thyA [H]

Identifier: 161870554

GI number: 161870554

Start: 1648605

End: 1649300

Strand: Reverse

Name: thyA [H]

Synonym: NMCC_1620

Alternate gene names: 161870554

Gene position: 1649300-1648605 (Counterclockwise)

Preceding gene: 161870557

Following gene: 161870553

Centisome position: 76.59

GC content: 52.73

Gene sequence:

>696_bases
ATGCGTTTTGACTTGGGCAAAGGTTTTCCGCTGCTGACGACCAAAAAGCTGCATCTGCGTTCGATTATCCACGAGCTGCT
TTGGTTTCTCAAAGGCGATACCAATATCAAATACCTAAAGGACAACAATGTTTCCATTTGGGACGAGTGGGCGGACGAAA
ACGGCGACTTAGGCCCGGTTTACGGCTACCAGTGGCGTAACTGGCCCGCGCCCGACGGCAGGCATATCGACCAAATCGCC
AATGTGCTGGAACAAATCAAGAAGAATCCCGACTCGCGCCGCCTGATTGTATCGGCGTGGAATCCGGCTTTGGTCGATGA
AATGGCCTTGCCGCCCTGCCACACGCTGTTTCAGTTTTACGTTGCCGACGGCAAACTGTCCTGCCAGCTTTACCAGCGTA
GTGCAGATATTTTCCTTGGTGTGCCGTTTAATATTGCCAGCTACGCGCTGTTGACGATGATGATGGCGCAAGTGTGCGGG
CTGGAGGCGGGCGAGTTTGTCCATACGTTTGGCGACGCGCATTTGTACCGCAACCATTTCGAGCAGGCCGCATTGCAGTT
GGAACGCGAACCGCGCGCCTTGCCAGTGATGAAAATCAATCCTGAAGTCAAAGATTTGTTTTCCTTCAAGTTTGAAGATT
TCGAGTTGGAAGGCTACGATCCGCATCCGCACATCAAGGCGGCAGTGTCGGTTTAG

Upstream 100 bases:

>100_bases
TATGAAAGCCTATTTGGACTTGATGCGCCACGTCCTCGACAACGGTACGGACAAATCAGACCGCACCGGTACGGGTACGC
GCTCCGTATTCGGTTATCAG

Downstream 100 bases:

>100_bases
GAGGCGGTATGGGCAGGCATTTCGGGCGCAGGCGTTTTTTGACGGCTGCCGCCGTTGCGGCGGCGGGCGCGGCGGTTTCT
TTTTTGCCGAATCCTTTTGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRFDLGKGFPLLTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWPAPDGRHIDQIA
NVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCG
LEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV

Sequences:

>Translated_231_residues
MRFDLGKGFPLLTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWPAPDGRHIDQIA
NVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCG
LEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV
>Mature_231_residues
MRFDLGKGFPLLTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWPAPDGRHIDQIA
NVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCG
LEAGEFVHTFGDAHLYRNHFEQAALQLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=250, Percent_Identity=51.2, Blast_Score=263, Evalue=9e-71,
Organism=Escherichia coli, GI1789191, Length=231, Percent_Identity=71.4285714285714, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71993377, Length=243, Percent_Identity=48.559670781893, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6324648, Length=227, Percent_Identity=49.3392070484581, Blast_Score=214, Evalue=1e-56,
Organism=Drosophila melanogaster, GI17137556, Length=253, Percent_Identity=52.1739130434783, Blast_Score=263, Evalue=1e-70,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 26591; Mature: 26591

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLGKGFPLLTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPV
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCC
YGYQWRNWPAPDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFY
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHH
VADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHF
HCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EQAALQLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV
HHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCEEECCCCCCCCCEEEECC
>Mature Secondary Structure
MRFDLGKGFPLLTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPV
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCC
YGYQWRNWPAPDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHTLFQFY
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHH
VADGKLSCQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHF
HCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EQAALQLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV
HHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCEEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9228757 [H]