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The map label for this gene is spoT [H]

Identifier: 161870506

GI number: 161870506

Start: 1597912

End: 1600068

Strand: Reverse

Name: spoT [H]

Synonym: NMCC_1569

Alternate gene names: 161870506

Gene position: 1600068-1597912 (Counterclockwise)

Preceding gene: 161870507

Following gene: 161870501

Centisome position: 74.3

GC content: 50.07

Gene sequence:

>2157_bases
ATGCCTGTCCCTCAACCCACCGCACCTTACGACCCTCTGACCGCCGAAGCGCGTACCCTGCTTTTCCATACAGCCTCCTA
CCTCAAGCCCGAGGAACAGGCGGAGCTTGAAAAAGCCGTCGCCTATGCGTTTCGCGCCCACGACGGACAAACCCGGAAAA
GTGGCGAACCATACATTACGCATCCAATTGCCGTTGCGACGCAGCTCGCCCTTTGGCATATGGACATACAGGGTCTGTGT
GCAGGCGTGATGCACGACGTATTGGAAGATACGGGCGTGACAAAAGGGGAAATGGCGGAGGTGTTCGGCAATACGATTGC
CGAGATGGTGGACGGTCTGTCCAAACTCGAAAAACTCAAATTTGAAGATCATGCGGAACATCAGGCGGAGAGTTTCCGCA
AACTGATTTTGGCAATGACCAAAGATGTGCGCGTGATTGTCGTTAAACTTGCCGACCGTCTGCATAATATGCGGACACTC
GGTTCGATGCGCCCGGACAAACGCCGCCGTATTGCAAGGGAAACCCTTGAAATCTACGCACAGATTGCCAACCGTATAGG
TTTGAATAACGCATATCAAGAGCTTCAGGATTTATCGTTCCAAAACCTGCATCCCAACCGCTACGAGACTTTAAAAAAAG
CGATGGACAAGAGCCGGAAGAACCGGCAGGACGTTGTCGGCAAAGTCTTGCGCGCATTCGGCCAGCGGCTGGTAGGCGCG
AATATAGAGGCCAAAATCAAAGGCAGGGAAAAAAACCTGTACGGCATCCATCAGAAAATGATGGCGAAAAAGCTGCGCTT
TGCCGAGGTTATGGATATTTACGGTTTCCGCGTCATTGTCAACAGCATTCCAGCCTGTTATGCCGCACTCGGCGCATTGC
ACACCCTCTATCAGCCCAAGCCCGGGCGGTTCAAAGACTATATCGCCATTCCGAAAAGCAATGGGTATCAAAGTCTGCAT
ACGACTTTGGTCGGCCCTTACGGCTTGCCGATTGAAGTTCAGATACGTACCAAGGAAATGGATGCTGTTGCCGAAGGTGG
AATCGCCGGACATTGGAGCTATAAATCATATTCTAAGACGGTCGATCAGGCGGTGCTTCACACAAACCGGTGGCTGAAAA
ATATCTTAGATTTGCAGGCAAGCAGTGCCAATGCTATTGAGTTTCTCGAACACGTCAAAGTCGATTTGTTTCCGAACGAA
ATCTACATCCTTACGCCAAAAGGAAAAATCCTAACTTTGCCCAAAGGGGCAACGCCTGTCGATTTTGCTTATGCGGTGCA
TACCGATATCGGGCACAAAACCGTTGCCGCACGTATCAACAATATCATGATGCCGTTGCGTACGAAGCTCAAAACCGGCG
ATTCCGTCGAAATTATTACATCCGAACACGCCAAACCCAATCCCGCGTGGTTGAATTTCGCCGTGTCCGGCAGGGCGCGC
AGTGCCATACGCCAATATATTAAAAACCTTAACCGGCACGATGCGGTCGTTTTGGGAGAGAGCCTCTTACAAAAAGCCCT
GTCCAGTTTGCTGCCCAAAGATGTCCTGCTTTCAGACGGCATCAAAGAAAAATACCTTGCCGATCTCAACGACAAACAGA
CTTCATTTGAGGAAGTGCTGTACAACGTCGGTATGGGGCATACCCTGCCTGTTTATGTCGCCATGCATATTGCTGAGTTG
GCAGGGGAGCATTTCGGCAGCGAGGTTAGGCTCAGTTCCATTAAAGTCGATGGTCAGGAAAGCGGGCATATTCATTTTGC
AGAGTGCTGCCACCCTGTTCCCGGCGATTCCATCCGTTTGCTGTTGGTTAAGGGAAAAGGCATGATTATCCATAGGGATA
GCTGCCCGACGTTGTTGAAGTCCGATCCCGAACAGCAGCTGGATGCAGACTGGGAAAATATGAACGGGCAGAACTACCGT
GTCGGGCTTCAAGTCCAATCGGAAGACAGCCACGGCTTGCTGGCATTAATGGCGCAAGCGATTTCCGATTCCGGTGCGGA
CATTGAGTCGGTCGAAACACCGTCTAAATCCCAGTCGGGAACGGAAGGTTTTGTCGAATTCAAATTCTTATTGAAAGTCA
AGAATCTGAATCAATTGAATCAGATTATTCAAAATCTGCATAGTATTCCATATATCCGCAAAGTCATCAGAAGTTGA

Upstream 100 bases:

>100_bases
ATAAATAAATTCTGCCGGAAACGCACGTCGGCACATTCTGCCGCCGTGCAGTCCGACGGTTTGAAACGAAAAATAAAATA
TGTTCCGAAAGCCGTCTGAA

Downstream 100 bases:

>100_bases
AGCAGGTAAAGCGGGTTGCCTTTTGATTAACAGGCAACCCGCTTTATGGTTTATCCGTTCAATTGCTTATTTGTTCAAAT
ATACGGCAAGCCTTTCAACA

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophophohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MPVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAFRAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLC
AGVMHDVLEDTGVTKGEMAEVFGNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLRAFGQRLVGA
NIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLH
TTLVGPYGLPIEVQIRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHVKVDLFPNE
IYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKLKTGDSVEIITSEHAKPNPAWLNFAVSGRAR
SAIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAEL
AGEHFGSEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPEQQLDADWENMNGQNYR
VGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS

Sequences:

>Translated_718_residues
MPVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAFRAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLC
AGVMHDVLEDTGVTKGEMAEVFGNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLRAFGQRLVGA
NIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLH
TTLVGPYGLPIEVQIRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHVKVDLFPNE
IYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKLKTGDSVEIITSEHAKPNPAWLNFAVSGRAR
SAIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAEL
AGEHFGSEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPEQQLDADWENMNGQNYR
VGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS
>Mature_717_residues
PVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAFRAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCA
GVMHDVLEDTGVTKGEMAEVFGNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTLG
SMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLRAFGQRLVGAN
IEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHT
TLVGPYGLPIEVQIRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHVKVDLFPNEI
YILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKLKTGDSVEIITSEHAKPNPAWLNFAVSGRARS
AIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELA
GEHFGSEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPEQQLDADWENMNGQNYRV
GLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=699, Percent_Identity=45.3505007153076, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=680, Percent_Identity=29.5588235294118, Blast_Score=352, Evalue=7e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 80251; Mature: 80120

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAFRAHDGQTRKSGEPYIT
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
HPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAEVFGNTIAEMVDGLSKLEKLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTLGSMRPDKRRRIARETLEIYA
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLRAFGQRLVGA
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
NIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAALGALHTLYQPK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQIRTKEMDAVAEGGIAGHWSYKSYSKT
CCCCCCEEECCCCCCCHHHHHHEECCCCCCEEEEEECCHHHHHHCCCCEECCCCHHHHHH
VDQAVLHTNRWLKNILDLQASSANAIEFLEHVKVDLFPNEIYILTPKGKILTLPKGATPV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCEEEEECCCCCEEECCCCCCCC
DFAYAVHTDIGHKTVAARINNIMMPLRTKLKTGDSVEIITSEHAKPNPAWLNFAVSGRAR
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCHHH
SAIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGIKEKYLADLNDKQTSFEEVL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCCCHHHHHHHH
YNVGMGHTLPVYVAMHIAELAGEHFGSEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHCCCCCCCCEEE
LLVKGKGMIIHRDSCPTLLKSDPEQQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQA
EEEECCEEEEECCCCCHHHCCCCHHHHCCCHHCCCCCCEEEEEEEECCCCCHHHHHHHHH
ISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS
HCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
PVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAFRAHDGQTRKSGEPYIT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
HPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAEVFGNTIAEMVDGLSKLEKLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTLGSMRPDKRRRIARETLEIYA
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLRAFGQRLVGA
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
NIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAALGALHTLYQPK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQIRTKEMDAVAEGGIAGHWSYKSYSKT
CCCCCCEEECCCCCCCHHHHHHEECCCCCCEEEEEECCHHHHHHCCCCEECCCCHHHHHH
VDQAVLHTNRWLKNILDLQASSANAIEFLEHVKVDLFPNEIYILTPKGKILTLPKGATPV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCEEEEECCCCCEEECCCCCCCC
DFAYAVHTDIGHKTVAARINNIMMPLRTKLKTGDSVEIITSEHAKPNPAWLNFAVSGRAR
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCHHH
SAIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGIKEKYLADLNDKQTSFEEVL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCCCHHHHHHHH
YNVGMGHTLPVYVAMHIAELAGEHFGSEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHCCCCCCCCEEE
LLVKGKGMIIHRDSCPTLLKSDPEQQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQA
EEEECCEEEEECCCCCHHHCCCCHHHHCCCHHCCCCCCEEEEEEEECCCCCHHHHHHHHH
ISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS
HCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]