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The map label for this gene is insI1 [C]

Identifier: 161870466

GI number: 161870466

Start: 1548879

End: 1549712

Strand: Reverse

Name: insI1 [C]

Synonym: NMCC_1521

Alternate gene names: 161870466

Gene position: 1549712-1548879 (Counterclockwise)

Preceding gene: 161870470

Following gene: 161870461

Centisome position: 71.97

GC content: 49.64

Gene sequence:

>834_bases
ATGTCTTGGTGGGAATTTAGGGGATTTTGGGGAATTTTGCAAAGGTCTCGGACTATCAAACAGCGTAAGCGACAACCCTA
TAAGCTCGATTCGCAGCTGATTCAGCACATCGACACCCTTATCCGCCGCAAACTCAGTCCCGAACAAGTATGCGCCTACC
TGTGCAAACACCACCGGATCACGCTCCACCACAGCACCATTTACCGCTACCTTCGCCAAGACAAAAGCAACGGCAGCACG
TTGTGGCAACATCTCAGAATATGCAGCAAACCCTACCGCAAACGCTACGGCAGCACATGGACCAGAGGCAAAGTCCCCAA
CCGTGTCGGCATAGAAAACCGACCCGCTATCGTCGACCAGAAATCCCATATCGGCGATTGGGAAGCCGACACCATTGTCG
GCAAAGGACAGAAAAGCGCATTATTGACCTTGGTCGAACGCGTTACCCGCTACACCATCATCTGCAAATTGGATAGCCTC
AAAGCCGAAGACACTGCCCGGGCAGCTGTTAGGGCATTAAAGGCACATAAAGACAGGGTGCACACCATCACCATGGATAA
CGGCAAAGAGTTCTACCAACACACCAAAATAACCAAAGCATTGAAAGCGGAGACTTATTTTTGTCGCCCTTACCATTCTT
GGGAGAAAGGGCTGAATGAGAACACCAACGGACTCATCCGGCAATACTTCCCCAAACAAACCGATTTCCGTAACATCAGT
GATCGGGAGATACGCAGGGTTCAAGATGAGTTGAACCACCGGCCAAGAAAAACACTTGGCTACGAAACGCCAAGTGTTTT
ATTCTTGAATCTGTTCCAACCACTAATACACTAG

Upstream 100 bases:

>100_bases
ATCACCTGGTCCAACTTCAATAGCGGGAAACGGTCGATGTGTTTGGCAATCATGGCTTGGGCGGTTTGCTGGAAGAAGGT
GCTCATGAGAAATCTCCTAA

Downstream 100 bases:

>100_bases
TGTTGCACTTGAAATCCGAATCCAAGGGCTTTTGAATATTGCCGTTGCCCGACGTTCTGGATTCCCGCAACTTTTCGTCA
TTCCCGCGAAAGCGGGAATC

Product: IS30 family transposase

Products: NA

Alternate protein names: Transposon TN4551 [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSWWEFRGFWGILQRSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHRITLHHSTIYRYLRQDKSNGST
LWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQKSHIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSL
KAEDTARAAVRALKAHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNIS
DREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH

Sequences:

>Translated_277_residues
MSWWEFRGFWGILQRSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHRITLHHSTIYRYLRQDKSNGST
LWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQKSHIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSL
KAEDTARAAVRALKAHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNIS
DREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH
>Mature_276_residues
SWWEFRGFWGILQRSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHRITLHHSTIYRYLRQDKSNGSTL
WQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQKSHIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLK
AEDTARAAVRALKAHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNISD
REIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=231, Percent_Identity=36.3636363636364, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790736, Length=231, Percent_Identity=36.3636363636364, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787671, Length=231, Percent_Identity=36.3636363636364, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32911; Mature: 32780

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWWEFRGFWGILQRSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHRI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCE
TLHHSTIYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQ
EEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
KSHIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARAAVRALKAHKDRV
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHE
HTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNIS
EEEEECCCHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCC
DREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH
HHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
SWWEFRGFWGILQRSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHRI
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCE
TLHHSTIYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQ
EEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
KSHIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARAAVRALKAHKDRV
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHE
HTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNIS
EEEEECCCHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCC
DREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH
HHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2820936 [H]