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The map label for this gene is gshB [H]

Identifier: 161870413

GI number: 161870413

Start: 1488374

End: 1489330

Strand: Reverse

Name: gshB [H]

Synonym: NMCC_1461

Alternate gene names: 161870413

Gene position: 1489330-1488374 (Counterclockwise)

Preceding gene: 161870414

Following gene: 161870412

Centisome position: 69.16

GC content: 54.96

Gene sequence:

>957_bases
ATGAAAGTCCTCTTTATCGCCGACCCGATGGCAAGTTTCAAAACCTACAAAGACACCACCTACGCCATGATGCGCGAGAT
GGCAAAACGCGGTTGGCGGCTGTTTCATACCTTGAGCGGGGAATTGTCGGTTAAAAACGGTCTGGTGGTTGCTGATGCGT
CTGCTTTTGAGTTTCTCGGCGCACAAGACGACCACGACAAAAACTGGTTTGCCGCCGCCGACAAAGTTCAGACGGCATTG
AAAGCATTTGATGCCGTGATTATGCGTACCGATCCGCCGTTCGATATGCAATACCTCTACGCCACCCAATTACTGACGCT
GGCGGAACAGCAGGGCGCGAAAGTCTTTAACAGCGGACAGTCGATGCGTGACTTCAATGAAAAACTGGCGATTTTGAACT
TCAGCCGCTTTACCGCGCCCACGCTGGTAACGACCCGTTCCGCCGATGTCCGCGCATTTTTGAAAGAACACGGCGACATT
ATCATCAAACCGCTGGACGGTATGGGCGGTATGGGTATTTTCCGCCTGACCGAAAAAGACCCCAACATCGGCAGCATCCT
CGAAACCCTGATGCGGCTTGATTCCCGCACCATTATGGCGCAACGCTACATTCCCGAAATCGTACACGGTGACAAACGCA
TCCTGATTATCGGCGGCGAAGTCGTCCCCTATGCTTTGGCGCGTATCCCGCAAAACGGCGAAACACGCGGCAACCTTGCG
GCAGGCGGACGCGGCGTGGCGCAGGAATTGGACGGACGCGACCGCGAAATTGCCGAAACGCTCGCCCCCGAACTCAAACG
GCGCGGCATCCTGCTGGCCGGTTTGGACGTTATCGGCAGCAACCTGACCGAAGTCAACGTAACCAGCCCGACCGGATTCC
AAGAAATTATGAAACAAAAAGGTTTCGACGTGGCGGCAATGTTTGCCGATGCCGTTGCCGCGTGGTCGGTACGTTAA

Upstream 100 bases:

>100_bases
CTAAGTAAAACCCCATCCTTGCCGTCTGAATATTGTTCGGACGGCATTTCCCCTTTGCCCGCGAAATGCGGCACATTCGG
CACACCGGAAAGGAAATATG

Downstream 100 bases:

>100_bases
ACCGATGCCGTTTGAAAGGCTTTTGCTTCACAACCGTTTGGTTTGTTCGGGCAGGCAGGGTTTTCCCTCAGCCGGCAGGG
ATGCGGTTAATGCCGTACGG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSVKNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTAL
KAFDAVIMRTDPPFDMQYLYATQLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIGGEVVPYALARIPQNGETRGNLA
AGGRGVAQELDGRDREIAETLAPELKRRGILLAGLDVIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR

Sequences:

>Translated_318_residues
MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSVKNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTAL
KAFDAVIMRTDPPFDMQYLYATQLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIGGEVVPYALARIPQNGETRGNLA
AGGRGVAQELDGRDREIAETLAPELKRRGILLAGLDVIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR
>Mature_318_residues
MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSVKNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTAL
KAFDAVIMRTDPPFDMQYLYATQLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIGGEVVPYALARIPQNGETRGNLA
AGGRGVAQELDGRDREIAETLAPELKRRGILLAGLDVIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=45.5414012738853, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35114; Mature: 35114

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSVKNGLVVADASAFEFLG
CEEEEEECCHHHHHHHCHHHHHHHHHHHHHHHEEEEHHCCCEEECCCEEEECCHHHHHCC
AQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYATQLLTLAEQQGAKVFNSGQ
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCC
SMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDIIIKPLDGMGGMGIFRLTEKD
HHHHHHHHEEEEEEHHHCCCEEEEECCHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCC
PNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIGGEVVPYALARIPQNGETRGNLA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCC
AGGRGVAQELDGRDREIAETLAPELKRRGILLAGLDVIGSNLTEVNVTSPTGFQEIMKQK
CCCCCHHHHCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCCCEEEEECCCCCHHHHHHHC
GFDVAAMFADAVAAWSVR
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSVKNGLVVADASAFEFLG
CEEEEEECCHHHHHHHCHHHHHHHHHHHHHHHEEEEHHCCCEEECCCEEEECCHHHHHCC
AQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYATQLLTLAEQQGAKVFNSGQ
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCC
SMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDIIIKPLDGMGGMGIFRLTEKD
HHHHHHHHEEEEEEHHHCCCEEEEECCHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCC
PNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIGGEVVPYALARIPQNGETRGNLA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCC
AGGRGVAQELDGRDREIAETLAPELKRRGILLAGLDVIGSNLTEVNVTSPTGFQEIMKQK
CCCCCHHHHCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCCCEEEEECCCCCHHHHHHHC
GFDVAAMFADAVAAWSVR
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]