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The map label for this gene is rph [H]

Identifier: 161870362

GI number: 161870362

Start: 1429601

End: 1430329

Strand: Reverse

Name: rph [H]

Synonym: NMCC_1406

Alternate gene names: 161870362

Gene position: 1430329-1429601 (Counterclockwise)

Preceding gene: 161870363

Following gene: 161870361

Centisome position: 66.42

GC content: 55.56

Gene sequence:

>729_bases
ATGCCTGATTACATCCGCATCTCGCGCGCCGCGGACAGCCTGCGCGATATCAAAATCACCCCTCATTTCCTGCCGCATAC
GGACGGTTCGTGCCTTATTGAATGCGGCAATACCAAAGTGATTTGTACCGCTTCCATAGATGAAAACGTCCCGCCGTTTC
TGCGCGGTAAAAACCAAGGCTGGGTAACGGCGGAATACGGGATGCTGCCTGCCTCAACTGCTTCCCGGATGCGCCGCGAA
GCTTCGGCGGGCAAACAGTCCGGGCGCACACAAGAAATCCAACGCCTTATCGGGCGTTCGCTGCGCGCGGTCGTGGATAT
GGAAAAACTCGGCGAACGACAAATCTTGATTGACTGCGATGTGATTCAGGCAGATGGCGGTACGCGCACGGCTTCGATTA
CCGGTGCGTTTGTCGCGCTGCAAATCGCCGTCGGCAAACTGGTTTCAGACGGCATTTTGAGTGAAAACCCCATCCGCGAA
GCCGTTGCCGCTGTGTCAGTAGGCGTAGTGAACGGCGTGCCGCTTTTGGATTTGGATTATCCCGAAGACTCCGGTTGCGA
CAGTGATGTGAATATCGTCATGACCGCATCGGGAAAAATCATCGAAATCCAAGGTACGGCGGAAGACGCGCCGTTCAGTT
TGGACGAATTGGGCAAACTGGTTGCACTGGCGCAAAAAGGCATAGGCGAACTGCTGCAACACCAGCAAAATGCGTTGTCC
GCCGCCTGA

Upstream 100 bases:

>100_bases
CAAAGCCGAAGAGGCGTAGAAATTTTCAGTTTGAAGGCCTTAAATGTGATTTTTCATATTTAAGGCTTTTTAATTTATCT
GTTTTTAACGGGAGGGCAGT

Downstream 100 bases:

>100_bases
TTGTCGTCCGGATGCCGTCTGAAAACCGTTCAGACGGCATTTTGTCAACAATCTGAAAGAACAGGTTTTTCGCTGACCGC
TGTCAAACGGCAGGCGGGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTASIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRRE
ASAGKQSGRTQEIQRLIGRSLRAVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELGKLVALAQKGIGELLQHQQNALS
AA

Sequences:

>Translated_242_residues
MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTASIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRRE
ASAGKQSGRTQEIQRLIGRSLRAVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELGKLVALAQKGIGELLQHQQNALS
AA
>Mature_241_residues
PDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTASIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREA
SAGKQSGRTQEIQRLIGRSLRAVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIREA
VAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELGKLVALAQKGIGELLQHQQNALSA
A

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=205, Percent_Identity=60, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25741; Mature: 25609

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTASIDENVPPFLRGKNQG
CCCCEEHHHHHCCHHEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCCCCHHHCCCCCC
WVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLRAVVDMEKLGERQILIDCD
EEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIREAVAAVSVGVVNGVPLLDLDY
EEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECC
PEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELGKLVALAQKGIGELLQHQQNALS
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
AA
CC
>Mature Secondary Structure 
PDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTASIDENVPPFLRGKNQG
CCCEEHHHHHCCHHEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCCCCHHHCCCCCC
WVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLRAVVDMEKLGERQILIDCD
EEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIREAVAAVSVGVVNGVPLLDLDY
EEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECC
PEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELGKLVALAQKGIGELLQHQQNALS
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA