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The map label for this gene is purK [H]

Identifier: 161869931

GI number: 161869931

Start: 963227

End: 964363

Strand: Reverse

Name: purK [H]

Synonym: NMCC_0963

Alternate gene names: 161869931

Gene position: 964363-963227 (Counterclockwise)

Preceding gene: 161869932

Following gene: 161869930

Centisome position: 44.78

GC content: 52.42

Gene sequence:

>1137_bases
ATGAAAAACATATCTCTTTCTCCGCCCGCCATGCTCGGCATTCTTGGCGGCGGACAATTAGGCAGAATGTTTACTGTTGC
TGCCAAAACCATGGGCTACAAAGTAACCGTTCTCGACCCCGATCCGGACGCGCCGGCAGCAGAATTTGCCGACTGTCATT
TATGCGCGCCGTTTAACGACCAAGCGGCTTTGGATGAATTGGCAAAATGCGCGGCGGTGACCACTGAATTTGAAAACGTC
AATGCCGATGCGATGCGCTTTTTGGCAAAACATACCAATGTTTCCCCTAGCGGCGATTGTGTGGCGATTGCACAAAACCG
CATTCAGGAAAAGGCATGGATACGCAAAGCGGGATTGCAAACCGCGCCGTATCAAGTGGTTTGTAAGGCTGAAGACATCA
CTGAAGCAAGCGCGCAATTTTTGCCCGGCATCCTGAAAACGGCTACGTTGGGCTACGACGGCAAAGGTCAAATCCGCGTA
AAAACATTGGATGAACTCAAAGCCGCGTTTACCGAACACGGCGGCGTGGATTGCGTTTTGGAAAAAATGGTGGATTTGCG
CAGTGAGATTTCCGTAATTGTATGCCGTTTGAACAATGACAACGTGCAAACTTTCGACCCTGCCGAAAACATCCACGAAA
ACGGCATCTTGGCTTATTCCATCGTCCCCGCGCGACTGAGTGCCGACGTGCAGCAACAGGCGCGGCAGATGGCGCAACGC
TTGGCAGACGAATTGGATTATGTCGGCGTATTGGCGGTAGAAATGTTTGTTGTCGGTGACACACATGAATTGGTCGTCAA
CGAAATCGCGCCGCGTCCGCACAACTCCGGCCACCATACCGTCGATGCCTGCGCGGCAGACCAATTCCAGCAACAGGTCC
GCCTGATGTGCAACCTGCCACCTGCTGACACCAAATTGCTGAGTTCTTGCTGTATGGCGAATATTTTGGGCGACGTTTGG
CAGGAAGACGGCGGCGAACCGGATTGGTTTCCCCTGCAAAGCCGGCCGGACGCGCACCTGCACCTTTACGGCAAAAAAAC
CGCGCACAAAGGGCGGAAAATGGGACACTTTACCGTTTTAACCACCGATTCGGACACCGCATTTCAAGAAGCAAAAAAAC
TGCATCAGTCCCTATAA

Upstream 100 bases:

>100_bases
AAAAAGTATTCTGTATTTCTGAAACTGGTAATTCCAGCGATACAAGACATCCGTCAAGAATTAGTTATAGCTATCAACTT
GTACAATTTGAAGAGTCAGC

Downstream 100 bases:

>100_bases
TCCGACCAACGGCACAGATGCCGTCTGAAGCCGCCGACATACTGCCATGAACCTGCTGACACTGTTACGCCCCGCCACCG
TGCAAGACTGCAAAGACATC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDAPAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENV
NADAMRFLAKHTNVSPSGDCVAIAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
KTLDELKAAFTEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADVQQQARQMAQR
LADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNSGHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVW
QEDGGEPDWFPLQSRPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL

Sequences:

>Translated_378_residues
MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDAPAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENV
NADAMRFLAKHTNVSPSGDCVAIAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
KTLDELKAAFTEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADVQQQARQMAQR
LADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNSGHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVW
QEDGGEPDWFPLQSRPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL
>Mature_378_residues
MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDAPAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENV
NADAMRFLAKHTNVSPSGDCVAIAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
KTLDELKAAFTEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADVQQQARQMAQR
LADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNSGHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVW
QEDGGEPDWFPLQSRPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=357, Percent_Identity=32.2128851540616, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788155, Length=358, Percent_Identity=25.6983240223464, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=31.1475409836066, Blast_Score=182, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41193; Mature: 41193

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDAPAAEFADCHLCAPFND
CCCCCCCCCHHEEEECCCCHHHHHHHHHHHHCEEEEEECCCCCCCHHHHCCCEEECCCCC
QAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVAIAQNRIQEKAWIRKAGLQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCC
TAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRVKTLDELKAAFTEHGGVDCVL
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHH
EKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADVQQQARQMAQR
HHHHHHHCCEEEEEEEECCCCCEEECCHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHH
LADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNSGHHTVDACAADQFQQQVRLMCNLP
HHHHHHHHHHEEEEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PADTKLLSSCCMANILGDVWQEDGGEPDWFPLQSRPDAHLHLYGKKTAHKGRKMGHFTVL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCEEEEE
TTDSDTAFQEAKKLHQSL
ECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDAPAAEFADCHLCAPFND
CCCCCCCCCHHEEEECCCCHHHHHHHHHHHHCEEEEEECCCCCCCHHHHCCCEEECCCCC
QAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVAIAQNRIQEKAWIRKAGLQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCC
TAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRVKTLDELKAAFTEHGGVDCVL
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHH
EKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADVQQQARQMAQR
HHHHHHHCCEEEEEEEECCCCCEEECCHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHH
LADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNSGHHTVDACAADQFQQQVRLMCNLP
HHHHHHHHHHEEEEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PADTKLLSSCCMANILGDVWQEDGGEPDWFPLQSRPDAHLHLYGKKTAHKGRKMGHFTVL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCEEEEE
TTDSDTAFQEAKKLHQSL
ECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]