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The map label for this gene is dut

Identifier: 161869825

GI number: 161869825

Start: 857675

End: 858127

Strand: Direct

Name: dut

Synonym: NMCC_0854

Alternate gene names: 161869825

Gene position: 857675-858127 (Clockwise)

Preceding gene: 161869824

Following gene: 161869826

Centisome position: 39.83

GC content: 51.88

Gene sequence:

>453_bases
ATGAATATTGAAGTAGAAATGAAAGTATTGGACGAGAGGATGGCGGATTTTATCCCTGCCTATGCAACGGAGGGTTCTGC
AGGTTTGGACTTGCGTGCCTGTTTGGATGAGGAAGTCGTTTTACAGCCGGGTGAAACGTTTCTTGTGCCGACGGGTTTGG
CAATTTATTTGGCGAATCCCGCATATGCCGCCGTTTTGCTGCCCCGTTCCGGCTTGGGGCATAAACACGGCATTGTCTTG
GGCAATTTGGTCGGTTTGATTGACTCCGATTATCAAGGGGAATTGAAGGTGTCGTTATGGAACAGGGGCAGTGAACCTTT
TGCCGTCAAACCGTTCGAGCGTATCGCACAGATGGTTATCGTGCCAGTTGTGCAGGCGGGCTTCAAACGTGTCGAGGAGT
TTGTCGGAAGCAGCCGGGGTGAGGGCGGCTTCGGCAGTACGGGTTCTCACTAA

Upstream 100 bases:

>100_bases
AACGGATTTCAAAACAAAACCGATTTGCCGTGTTTCAGCGTAAACACGGCTTGTGTATAATCTCCCATCTTTGAAACCGA
CCGTATGCAGGAGCAAGACG

Downstream 100 bases:

>100_bases
AAATATAGAATGCCGTCTGAAAGACACGTCAGGTTCAGACGGCATATCTTCCCAATATGCCGGTAATCGGAGAACATTAT
GAATACCTTACTCAACCAAC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPGETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVL
GNLVGLIDSDYQGELKVSLWNRGSEPFAVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH

Sequences:

>Translated_150_residues
MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPGETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVL
GNLVGLIDSDYQGELKVSLWNRGSEPFAVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH
>Mature_150_residues
MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPGETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVL
GNLVGLIDSDYQGELKVSLWNRGSEPFAVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=38.6363636363636, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=38.6363636363636, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=38.6363636363636, Blast_Score=77, Evalue=8e-15,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=64.4295302013423, Blast_Score=202, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71988561, Length=133, Percent_Identity=41.3533834586466, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=139, Percent_Identity=35.2517985611511, Blast_Score=74, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24583610, Length=130, Percent_Identity=36.9230769230769, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921126, Length=130, Percent_Identity=36.9230769230769, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_NEIG1 (Q5F9E0)

Other databases:

- EMBL:   AE004969
- RefSeq:   YP_207609.1
- ProteinModelPortal:   Q5F9E0
- SMR:   Q5F9E0
- STRING:   Q5F9E0
- EnsemblBacteria:   EBNEIT00000002224
- GeneID:   3282986
- GenomeReviews:   AE004969_GR
- KEGG:   ngo:NGO0459
- NMPDR:   fig|242231.4.peg.27
- eggNOG:   COG0756
- GeneTree:   EBGT00050000021459
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q5F9E0
- ProtClustDB:   PRK00601
- BioCyc:   NGON242231:NGO0459-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16104; Mature: 16104

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPGETFLVPTGLAIYLANP
CCEEEHHHHHHHHHHHHHCHHCCCCCCCCCHHHHCCCCEEECCCCEEEECCCEEEEEECC
AYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNRGSEPFAVKPFERIAQMVI
CEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECHHHHHHHHHH
VPVVQAGFKRVEEFVGSSRGEGGFGSTGSH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPGETFLVPTGLAIYLANP
CCEEEHHHHHHHHHHHHHCHHCCCCCCCCCHHHHCCCCEEECCCCEEEECCCEEEEEECC
AYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNRGSEPFAVKPFERIAQMVI
CEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECHHHHHHHHHH
VPVVQAGFKRVEEFVGSSRGEGGFGSTGSH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA