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The map label for this gene is radA [H]

Identifier: 161869728

GI number: 161869728

Start: 752273

End: 753652

Strand: Direct

Name: radA [H]

Synonym: NMCC_0748

Alternate gene names: 161869728

Gene position: 752273-753652 (Clockwise)

Preceding gene: 161869724

Following gene: 161869733

Centisome position: 34.93

GC content: 58.7

Gene sequence:

>1380_bases
ATGGCAAAAACGCTTAAAACCCTTTACCAATGCACCGAATGCGGCGGCACTTCCCCGAAATGGCAGGGCAAATGCCCGCA
TTGCGGCGAGTGGAACACACTTCAGGAAAGCTTAGCCGCGCCCGAGCCGAAAAATGCCCGTTTCCAATCTTGGGCGGCGG
ACACTTCGACCGTCCAATCCCTCTCCGCCGTTACCGCCACCGAAGTGCCGCGCAATCCGACCGGCATGGGCGAACTCGAC
CGCGTATTGGGCGGCGGTTTGGTCGACGGTGCGGTCATCCTGCTCGGCGGCGACCCCGGCATCGGCAAATCCACGCTGCT
GCTGCAAACCATCGCCAAAATGGCGCAAAGCCGCAAAGTACTGTACGTTTCCGGCGAGGAATCCGCCCAACAGGTCGCCC
TGCGCGCGCAACGTTTGGAACTGCCGACCGAAGGCGTGAACCTGCTTGCCGAAATCCGCATGGAAGCCATTCAGACGGCC
TTGAAACAGCATCAGCCCGAAGTCGTCGTCATCGACTCCATCCAAACCATGTATTCCGACCAAATCACTTCCGCCCCTGG
TTCCGTGTCGCAGGTGCGCGAGTGTGCCGCCCAACTGACGCGCATGGCGAAACAGATGGGCATCGCCATGATACTGGTCG
GACACGTGACCAAAGACGGTGCGATTGCCGGTCCGCGCGTGCTGGAGCATATGGTCGATACCGTGCTGTATTTCGAGGGC
GACCAGCATTCCAACTACCGCATGATACGCGCCATCAAAAACCGCTTCGGCGCGGCAAACGAACTGGGCGTATTCGCCAT
GACCGAAAACGGTTTGAAAGGCGTGTCCAACCCGTCCGCCATCTTCCTTGCCAGCTACCGCGACGACACGCCCGGTTCGT
GCGTTTTGGTTACGCAGGAAGGCAGCCGACCGCTTTTGGTTGAAATTCAGGCATTGGTCGATGACGCGCACGGATTCACC
CCCAAACGCCTTACCGTCGGACTGGAACAAAACCGCCTTGCGATGCTGCTTGCCGTGTTAAACCGACACGGCGGCATCGC
CTGTTTCGACCAAGACGTGTTTCTCAATGCCGTCGGCGGCGTGAAAATCGGCGAACCGGCGGCGGATTTGGCAGTCATCC
TCGCGATGCTTTCCAGCTTCCGCAACCGCCCCATGCCCGAAAAAACCGTGGTTTTCGGCGAAATCGGCTTAAGCGGCGAA
GTCCGCCCTGTCGCACGCGGACAAGAGCGGCTCAAAGAAGCGGAAAAACTCGGCTTCAAACGCGCCATTGTTCCCAAAGC
CAATATGCCGCGCAACGCCAAAGAGTTTCCGAACCTGAAAATCTACGGCGTTTCGAGTTTGCAGGAAGCCATCGATATTT
GCCGCGACAGCAGGGAATAA

Upstream 100 bases:

>100_bases
GTCTTGTAATCAAAATTAACAAACTAATAATAAAAAATTTTTTAGAAAAGAAGTTTCAGACGATTTTTTCACACTTCAAA
AATCCCATAGGGAAAACATC

Downstream 100 bases:

>100_bases
ACGGAAATGCCGTCTGAAATCGGGTTTCAGACGGCATTTGATTTCCATCCGACATATGTCGGCGACGGGCTTATTCGCCT
ACGGTTTTTTGTATCAGTTT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPKNARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVSNPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVFGEIGLSGE
VRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEAIDICRDSRE

Sequences:

>Translated_459_residues
MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPKNARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVSNPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVFGEIGLSGE
VRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEAIDICRDSRE
>Mature_458_residues
AKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPKNARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDR
VLGGGLVDGAVILLGGDPGIGKSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTAL
KQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTKDGAIAGPRVLEHMVDTVLYFEGD
QHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVSNPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTP
KRLTVGLEQNRLAMLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVFGEIGLSGEV
RPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEAIDICRDSRE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=461, Percent_Identity=56.6160520607375, Blast_Score=529, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49578; Mature: 49447

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPKNARFQSWAADTSTVQS
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCCHHHHHH
LSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIGKSTLLLQTIAKMAQSRKV
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEE
LYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTALKQHQPEVVVIDSIQTMYSD
EEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
QITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTKDGAIAGPRVLEHMVDTVLYFEG
HHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHEEEEC
DQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVSNPSAIFLASYRDDTPGSCVLVTQE
CCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECC
GSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLAMLLAVLNRHGGIACFDQDVFLNAVGG
CCCCEEEEHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHCCCC
VKIGEPAADLAVILAMLSSFRNRPMPEKTVVFGEIGLSGEVRPVARGQERLKEAEKLGFK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
RAIVPKANMPRNAKEFPNLKIYGVSSLQEAIDICRDSRE
EEECCCCCCCCCHHHCCCEEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPKNARFQSWAADTSTVQS
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCCHHHHHH
LSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIGKSTLLLQTIAKMAQSRKV
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEE
LYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTALKQHQPEVVVIDSIQTMYSD
EEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
QITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTKDGAIAGPRVLEHMVDTVLYFEG
HHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHEEEEC
DQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVSNPSAIFLASYRDDTPGSCVLVTQE
CCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECC
GSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLAMLLAVLNRHGGIACFDQDVFLNAVGG
CCCCEEEEHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHCCCC
VKIGEPAADLAVILAMLSSFRNRPMPEKTVVFGEIGLSGEVRPVARGQERLKEAEKLGFK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
RAIVPKANMPRNAKEFPNLKIYGVSSLQEAIDICRDSRE
EEECCCCCCCCCHHHCCCEEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]