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The map label for this gene is hemX [H]

Identifier: 161869722

GI number: 161869722

Start: 744104

End: 745438

Strand: Direct

Name: hemX [H]

Synonym: NMCC_0742

Alternate gene names: 161869722

Gene position: 744104-745438 (Clockwise)

Preceding gene: 161869721

Following gene: 161869723

Centisome position: 34.55

GC content: 54.23

Gene sequence:

>1335_bases
GTGGGCGAACCTGAAAACAAATCATCCGAACCCGTACGCGAGATACAGGCATCAAAAGAAATGCCGTCTGAAACCTCTTC
CCCACGCAAAGAAAACGAAACAGAAGTACACATTCCTGCCGCTCCTTTTATCGTCAAACAGTCCGGCAGCAACGCTTTGG
CAGTCTGCGCCCTGGTATTGGCGGCATTGGGTTTGGGTACAAGTGGTTTTTTGTTTGTCCAAGGACAGAATGTCTTGAAA
AACCAAGAGCTGGCATTCAACCAAAAAATCGACAAAGCCGCCTTGGGCGAGTCGGAAAACGCCGCCCTGTTGAAAGACAA
CCTCAACCGGCAAGCCGCCATACAATCAGAGCTCGACCGTTTGGACGGAAACGTCAAAGCAAACGGCGAACAAATCTTGG
AAATGCAAAAATCCTATCGCGAGTTGACCAAAGGACGCGCCGATTGGCTGGTGGACGAAACCGAGACCATACTCAATCTG
GCGGCGCAACAGCTGGTGTTGACCGGCAATATCCAAACGGCAGTCGGCGTATTGGAGCATATCGACAGCCGCCTGTCCCG
TTTCGATCAGGCAGAGCTTCTGCCGATCAAACAGGCAGTCAGCAGCGACTTGGCGGAACTGAAAAACCGTCCCTATGTCG
ATATTTCCGGCACGGCATTGCGCCTCGACAGGCTGGAAACCGCCGTATCCGGACTGCCGCTGATTTTGGACGGCGTATTG
AAGCCGGGCGTACAGGTGAAGAACGAAGCCGCTTCTGCTTCATGGTGGCAGAACGTATGGGAAAAATCCCTCGGCACATT
GAAGGGGCTGGTCGAAATCCGCCGTTTGGAAAACAACGATGCCATGCTGATTTCTCCCGAACAGGCATATTTTGTGCGTG
AAAACCTGCGCCTCCGCCTTTTGGATGCGCGGACTGCATTAATGCAGCGCAACAGCGAAGTCTATCAGGGCGATTTGAAC
AATGCCGAAGCCGCCGTCAGACAGTATTTCGATGCCAAGTCTCCCGCCACGCAGTCGTGGCTGAAAGAACTGGCGGAATT
GAAGGCGTTGGATGTGCGCATGACGGCGGATGACGGTTTGAAAAACAGCCTAAATGCCGTCCGCGCCTATCGCGACGGTA
CGCGCATGACGGCGGCGGAAAATCAAGAAGCAGAACAGGCGGCTTCCGAACCGGCAAACGAAAACACAGCTTCCGAACCG
GCTGCCGCATCGGATGTGAAGACCATAGAAGCACCGTCCCTGCCTTCGGAACGCAAACCGGAACAGCCTGCAAAAAAACA
GCCCGTACCGGAAAAGGCAGGGCGTTCGCCGTCCGCTAAAGGAGAACGCGCATGA

Upstream 100 bases:

>100_bases
AAACCGTCCCTACGCTGGAAGCCGCGCTTTCCCATTCTTCCATTTCCGTTTCAGACGGCATGGTCTTTCCCGGAACCTCA
AATTAATAAGGAGCAAAACG

Downstream 100 bases:

>100_bases
AAACGGTAGTCTGGATTGTCGTCCTGTTTGCCGCCGCCGTCGGGCTGGCATTGGCTTCGGGCATTTACACCGGCGACGTG
TATATCGTACTCGGACAGAC

Product: uroporphyrin-III C-methyltransferase HemX, putative

Products: NA

Alternate protein names: Urogen-III methylase; ORF X [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLK
NQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDGVL
KPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLN
NAEAAVRQYFDAKSPATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEP
AAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA

Sequences:

>Translated_444_residues
MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLK
NQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDGVL
KPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLN
NAEAAVRQYFDAKSPATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEP
AAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA
>Mature_443_residues
GEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKN
QELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLA
AQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDGVLK
PGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNN
AEAAVRQYFDAKSPATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPA
AASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790235, Length=230, Percent_Identity=26.5217391304348, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 48512; Mature: 48381

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHH
AALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDR
HHHCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHH
LDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
HCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHH
IDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDGVL
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHH
KPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENLRLRL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCEEEE
LDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRMTADDGL
HHHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCH
KNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPAAASDVKTIEAPSLPSERKP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
EQPAKKQPVPEKAGRSPSAKGERA
CCCCCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
GEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHH
AALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDR
HHHCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHH
LDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
HCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHH
IDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDGVL
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHH
KPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENLRLRL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCEEEE
LDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRMTADDGL
HHHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCH
KNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPAAASDVKTIEAPSLPSERKP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
EQPAKKQPVPEKAGRSPSAKGERA
CCCCCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062586; 3054815; 1379743; 9278503; 9298646 [H]