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The map label for this gene is ampD [H]

Identifier: 161869605

GI number: 161869605

Start: 638660

End: 639232

Strand: Reverse

Name: ampD [H]

Synonym: NMCC_0621

Alternate gene names: 161869605

Gene position: 639232-638660 (Counterclockwise)

Preceding gene: 161869619

Following gene: 161869604

Centisome position: 29.68

GC content: 56.54

Gene sequence:

>573_bases
ATGGACAATCCCGCTACGGCACCCTGGCAGGACGGTTGGCTGCAAAGCGTGCGCCACATTCCGTCGCCGAATTTCGGCCC
GAGGGAGTCCAAAGAAGAGGTTTCCCTTGTCGTATTGCACAATATCTCGCTGCCGCCGTTCGAATATGGTACGGATGCGG
TGGAAAAGCTGTTTGCCAACCGGCTCGACCCCGACGGACATCCGTTCTTCAGCCTGATACACACTTTGCGCGTATCCAGC
CATTTTTTAATCAAACGCGACGGCGAAACGGTACAGTTCGTTTCCTGCGACAATATGGCATACCATGCCGGCGTATCCTC
GTTTCGCGGACGGGAAAAATGCAACGCATTTTCCATCGGCATCGAACTGGAAGGCTGCGATTTCGAACCCTTTACCGAAG
CGCAATACCGTTCGCTCGAAGCCTTGTTGGACGCAATCTGCCGCCGCTACCCCATCACGGCGGTAACGGGGCACCAAGAC
ATCGCGCCCAGCCGCAAAACCGACCCCGGTCATTTTTTCGACTGGCGGCGGATACGGGGGAAAGGGTTTCCCGTGGACAG
AAATGCCGTCTGA

Upstream 100 bases:

>100_bases
AACAATTTTCTCAACATGGTAGGATTCCCAATATCGGAATACATCTTAAAATTCGGGCGACAGTATAACCCAAATTACCC
CATAAGACAGAAAGGACATC

Downstream 100 bases:

>100_bases
AAACCACAGATTTTAGACGGCATTTCGGAAAATTTCCGCTTATTTCGACACACTGCTGTCAGACCCTGCAATTTTCGTTA
TAATTCAAACGTTATAACCG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISLPPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSS
HFLIKRDGETVQFVSCDNMAYHAGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
IAPSRKTDPGHFFDWRRIRGKGFPVDRNAV

Sequences:

>Translated_190_residues
MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISLPPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSS
HFLIKRDGETVQFVSCDNMAYHAGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
IAPSRKTDPGHFFDWRRIRGKGFPVDRNAV
>Mature_190_residues
MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISLPPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSS
HFLIKRDGETVQFVSCDNMAYHAGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
IAPSRKTDPGHFFDWRRIRGKGFPVDRNAV

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=54.0697674418605, Blast_Score=187, Evalue=3e-49,
Organism=Escherichia coli, GI1787092, Length=136, Percent_Identity=38.2352941176471, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21504; Mature: 21504

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISLPPFEYGTDAVEKLFAN
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
RLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYHAGVSSFRGREKCNAFSIG
CCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCHHHHCCHHHCCCCCCCEEEEE
IELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQDIAPSRKTDPGHFFDWRRIRG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCC
KGFPVDRNAV
CCCCCCCCCC
>Mature Secondary Structure
MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISLPPFEYGTDAVEKLFAN
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
RLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYHAGVSSFRGREKCNAFSIG
CCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCHHHHCCHHHCCCCCCCEEEEE
IELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQDIAPSRKTDPGHFFDWRRIRG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCC
KGFPVDRNAV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]