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The map label for this gene is hisH [H]

Identifier: 161869563

GI number: 161869563

Start: 602433

End: 603071

Strand: Reverse

Name: hisH [H]

Synonym: NMCC_0578

Alternate gene names: 161869563

Gene position: 603071-602433 (Counterclockwise)

Preceding gene: 161869564

Following gene: 161869562

Centisome position: 28.01

GC content: 53.36

Gene sequence:

>639_bases
ATGCAAACCGCCATTATCGATTACGGTATGGGCAACCTGCATTCCGTATTGAAATCCGTCCGGACGGCGGGGCAGCTTGC
CGGAAAAAATACCGAAATCTTTTTAAGCGGCGACCCCGACCGCGTGTCCCGCGCCGACAAAGTCATTTTTCCCGGTCAGG
GTGCGATGCCCGACTGTATGGCGGCATTAAAACGAGACGGTTTGGACGAGGCAGTCAAAGATGCCTTAAAAAACAAACCG
TTTTTCGGAATCTGCGTCGGCGCGCAACTTTTATTCGACCACAGCGAAGAAGGAAACACCGACGGCTTGGGCTGGTTCGG
CGGCAAAGTCAGACGCTTTGAGCGCGACCTCCGCGACCCGCAGGGATGCCGTCTGAAAGTCCCGCATATGGGCTGGAACA
CCGTGCGCCAAACCCAAAACCACCCGCTGTTTAAAGATATTCCCCAAGACACGCGTTTTTACTTCGTCCACAGCTACTAT
TTCGCCCCCGAAAATCCCGAAACCATATTGGGCGAAAGCGACTACCCGTCCCCGTTTGCCTGCATCGTCGGCAAAGACAA
CGTATTCGCCACGCAATTTCACACCGAAAAAAGCCACGATGCCGGGCTGACGATGTTGAAAAACTTTTTAAACTGGTAA

Upstream 100 bases:

>100_bases
CGTCTGAAGACAGATTCCCGTTTTCAGACGGCATTTTTATCAGCCCGGCACATTTGTTTGTTAAAATCGCAGCCATATTG
CAAAAAAAGAGGAGGAAGCC

Downstream 100 bases:

>100_bases
GCCGGACACGGCCCCGCACAAGGAGAAAAATTATGCTGCTGATACCCGCCATCGATTTGAAAGAAGGACGCTGCGTCCGC
CTGAGACAAGGGCTGATGGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSRADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKP
FFGICVGAQLLFDHSEEGNTDGLGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW

Sequences:

>Translated_212_residues
MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSRADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKP
FFGICVGAQLLFDHSEEGNTDGLGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW
>Mature_212_residues
MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSRADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKP
FFGICVGAQLLFDHSEEGNTDGLGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=38.8625592417062, Blast_Score=120, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23773; Mature: 23773

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSRADKVIFPGQGAMPDCM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCEEEECCCCCCHHHH
AALKRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDGLGWFGGKVRRFERDLRDP
HHHHHCCHHHHHHHHHHCCCEEEEEECHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCC
QGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYYFAPENPETILGESDYPSPFA
CCCEEECCCCCCHHHHHCCCCCCHHCCCCCCEEEEEEEEEECCCCCCEEECCCCCCCCEE
CIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW
EEEECCCEEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSRADKVIFPGQGAMPDCM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCEEEECCCCCCHHHH
AALKRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDGLGWFGGKVRRFERDLRDP
HHHHHCCHHHHHHHHHHCCCEEEEEECHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCC
QGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYYFAPENPETILGESDYPSPFA
CCCEEECCCCCCHHHHHCCCCCCHHCCCCCCEEEEEEEEEECCCCCCEEECCCCCCCCEE
CIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW
EEEECCCEEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA