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The map label for this gene is hisF [H]

Identifier: 161869561

GI number: 161869561

Start: 600883

End: 601650

Strand: Reverse

Name: hisF [H]

Synonym: NMCC_0576

Alternate gene names: 161869561

Gene position: 601650-600883 (Counterclockwise)

Preceding gene: 161869562

Following gene: 161869560

Centisome position: 27.94

GC content: 61.33

Gene sequence:

>768_bases
ATGGCACTGGCAAAACGCATCATCCCCTGTCTCGACGTAAAAGACGGGCGCGTCGTCAAAGGCGTGAACTTCATCGGTTT
GCGCGACGCGGGCGACCCCGTCGAAGCCGCCAAACGCTACAACGGCGAAGGCGCGGACGAATTGACCTTCCTCGACATCA
CCGCCTCATCCGACAACCGCGACACCATCCTGCACATCATCGAAGAGGTTGCCGGACAAGTCTTCATCCCGCTGACCGTC
GGCGGCGGCGTACGCACCGTTGCCGACATCCGCCGCCTGCTCAATGCCGGCGCGGACAAAGTCAGCATCAACACCGCCGC
CGTAACCCGTCCCGATTTAATTAACGAAGCCGCCGGATTTTTCGGTTCGCAAGCCATCGTCGCCGCCGTCGATGCCAAAG
CCGTCAACCCCGAAAACACACGCTGGGAAATCTTTACCCACGGCGGGCGCAATCCGACCGGTTTGGATGCGGTGGAATGG
GCGGTCGAAATGCAAAAACGCGGCGCGGGCGAAATCCTGCTCACCGGTATGGACAGGGACGGTACGAAACAGGGTTTCAA
CCTGCCGCTGACCCGCGCCGTTGCCGAAGCCGTCGACATTCCCGTCATCGCCTCCGGCGGGGTCGGCAATGTCCGGCACC
TGATTGAAGGCATAACCGAAGGCAAAGCCGATGCCGTACTTGCCGCCGGCATTTTCCATTTCGGGGAAATCGCCATCCGC
GAAGCCAAACGCGCTATGCGCGAAGCCGGCATCGAAGTGCGCCTCTGA

Upstream 100 bases:

>100_bases
CGTAGCAGGCGCGATTACCGGCCGCGCGATTTACGAGGGCAGCATCGATTTTGCCCAAGCGCAGCAACTGGCAGATTCCC
TCGACTAAAGGCATCCGATT

Downstream 100 bases:

>100_bases
CCGCCCCGACTATGCCGTCTGAAAGGAAATATGGATAAAAACCTGCTTGAAGCCGTCAAATTTGACGAAAAAGGTTTGGT
TTGCGCCATCGCCCAAGATG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEGADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTV
GGGVRTVADIRRLLNAGADKVSINTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGITEGKADAVLAAGIFHFGEIAIR
EAKRAMREAGIEVRL

Sequences:

>Translated_255_residues
MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEGADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTV
GGGVRTVADIRRLLNAGADKVSINTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGITEGKADAVLAAGIFHFGEIAIR
EAKRAMREAGIEVRL
>Mature_254_residues
ALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEGADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVG
GGVRTVADIRRLLNAGADKVSINTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEWA
VEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGITEGKADAVLAAGIFHFGEIAIRE
AKRAMREAGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.1906614785992, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI87082028, Length=209, Percent_Identity=30.622009569378, Blast_Score=87, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=35.8490566037736, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27028; Mature: 26897

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEGADELTFLDITASSDNR
CCCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCH
DTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVSINTAAVTRPDLINEAAGF
HHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHH
FGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEWAVEMQKRGAGEILLTGMDRD
CCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
GTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGITEGKADAVLAAGIFHFGEIAIR
CCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EAKRAMREAGIEVRL
HHHHHHHHCCCEECC
>Mature Secondary Structure 
ALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEGADELTFLDITASSDNR
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCH
DTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVSINTAAVTRPDLINEAAGF
HHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHH
FGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEWAVEMQKRGAGEILLTGMDRD
CCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
GTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGITEGKADAVLAAGIFHFGEIAIR
CCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EAKRAMREAGIEVRL
HHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA