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The map label for this gene is prfC [H]

Identifier: 161869559

GI number: 161869559

Start: 598755

End: 600326

Strand: Reverse

Name: prfC [H]

Synonym: NMCC_0574

Alternate gene names: 161869559

Gene position: 600326-598755 (Counterclockwise)

Preceding gene: 161869560

Following gene: 161869554

Centisome position: 27.88

GC content: 54.64

Gene sequence:

>1572_bases
GTGCGCCGCCGCCGCACGTTTGCCATCATCTCCCACCCTGACGCTGGTAAAACCACGTTGACCGAAAAGCTGTTGCTGTT
TTCAGGCGCGATTCAGAGTGCGGGTACGGTAAAAGGCAAGAAAACCGGCAAATTCGCCACCTCCGACTGGATGGAAATCG
AGAAGCAGCGCGGCATTTCCGTCGCTTCGTCAGTGATGCAGTTCGACTACAAAGACCACACCGTCAACCTCTTGGACACG
CCGGGACACCAAGACTTCTCCGAAGACACCTACCGCGTTTTGACCGCCGTGGACAGCGCATTGATGGTCATCGACGCGGC
AAAAGGCGTGGAAGCGCAAACCATCAAACTCTTAAACGTCTGCCGCCTGCGCGATACGCCGATTGTTACCTTTATGAACA
AATACGACCGCGAAGTGCGCGACTCGCTGGAACTCTTGGACGAAGTGGAAAACATCCTGCAAATCCGCTGCGCGCCCGTT
ACCTGGCCGATCGGTATGGGCAAAAACTTCAAAGGCGTATACCACATCCTGAACGATGAAATCTATCTCTTTGAAGCCGG
CGACGAACGCCTGCCGCACGAGTTCGACATCATCAAAGGCATAGACAATCCCGAATTGGAACAACGCTTTCCATTGGAAA
TACAGCAGTTGCGCGACGAAATCGAATTGGTACAGGCGGCTTCCAACGAGTTCAATCTCGACGAATTTCTCGCCGGCGAA
CTCACGCCCGTATTCTTCGGCTCTGCGATTAACAACTTCGGTATTCAGGAAATCCTCAATTCATTGATTGACTGGGCACC
CGCACCGAAACCGCGCGACGCGACCGTACGCATGGTCGAGCCGGACGAGGCTAAGTTTTCCGGATTTATCTTTAAAATCC
AAGCCAATATGGATCCGAAACACCGCGACCGCATCGCTTTTTTGCGCGTCTGCTCCGGTAAATTCGAGCGCGGCATGAAA
ATGAAACACCTGCGTATCAACCGCGAAATCGCCGCCTCCAGCGTGGTAACCTTCATGTCCCACGACCGCGAGCTGGTTGA
AGAAGCCTACGCCGGCGACATTATCGGTATCCCAAACCACGGCAACATCCAAATCGGCGACAGCTTCTCCGAAGGCGAAC
AACTGACGTTTACCGGCATCCCATTCTTCGCGCCCGAACTGTTCCGCAGCGTTCGCATCAAAAACCCGCTGAAAATCAAG
CAACTGCAAAAAGGTTTGCAACAGCTTGGCGAAGAAGGTGCGGTGCAGGTGTTCAAACCGATGAGCGGCGCGGATTTGAT
TTTGGGCGCGGTCGGCGTGTTGCAATTTGAAGTCGTTACCTCGCGGCTGGCCAACGAATACGGCGTAGAAGCCGTGTTCG
ACAGCGCATCCATCTGGTCGGCGCGCTGGGTATCGTGCGACGACAAGAAAAAACTGGCGGAATTTGAAAAAGCCAATGCA
GGCAACCTCGCCATCGACGCGGGCGGCAACCTCGCCTACCTCGCCCCTAACCGCGTGAATCTGGGACTCACGCAAGAACG
CTGGCCGGACATCGTGTTCCACGAAACGCGCGAGCATTCGGTCAAACTTTAA

Upstream 100 bases:

>100_bases
TTTTGTACAATTTCGCCGTCTCAAACACTGTCCGGGCCGTCTGAAAAGCGGCCTGAACTTTTTACAAAGAAAAACTATGT
CCCAAGAAATCCTCGACCAA

Downstream 100 bases:

>100_bases
AAAGCAATCGGCAATAAAATGCCGTCTGAACCCGAAAAAAGGGCTTCAGACGGCATTTTTGCCTGCAACTCAAACGCAGA
CGGTCAAAATCAAGGCGCAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDT
PGHQDFSEDTYRVLTAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENILQIRCAPV
TWPIGMGKNFKGVYHILNDEIYLFEAGDERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFNLDEFLAGE
LTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMK
MKHLRINREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFAPELFRSVRIKNPLKIK
QLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSRLANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANA
GNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKL

Sequences:

>Translated_523_residues
MRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDT
PGHQDFSEDTYRVLTAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENILQIRCAPV
TWPIGMGKNFKGVYHILNDEIYLFEAGDERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFNLDEFLAGE
LTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMK
MKHLRINREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFAPELFRSVRIKNPLKIK
QLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSRLANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANA
GNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKL
>Mature_523_residues
MRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDT
PGHQDFSEDTYRVLTAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENILQIRCAPV
TWPIGMGKNFKGVYHILNDEIYLFEAGDERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFNLDEFLAGE
LTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMK
MKHLRINREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFAPELFRSVRIKNPLKIK
QLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSRLANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANA
GNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=490, Percent_Identity=26.530612244898, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI19923640, Length=506, Percent_Identity=27.0750988142292, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI25306287, Length=481, Percent_Identity=26.8191268191268, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI25306283, Length=353, Percent_Identity=30.3116147308782, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=30.3571428571429, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=34.3065693430657, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=32.8859060402685, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=35.3982300884956, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=35.3982300884956, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=35.3982300884956, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790835, Length=518, Percent_Identity=64.0926640926641, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=497, Percent_Identity=27.1629778672032, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788922, Length=172, Percent_Identity=33.1395348837209, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994988, Length=137, Percent_Identity=35.036496350365, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=27.2727272727273, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=35.9550561797753, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=31.3333333333333, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=181, Percent_Identity=30.939226519337, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=27.348643006263, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=519, Percent_Identity=25.626204238921, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=153, Percent_Identity=28.7581699346405, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=166, Percent_Identity=31.3253012048193, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=166, Percent_Identity=31.3253012048193, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324166, Length=147, Percent_Identity=30.6122448979592, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582462, Length=496, Percent_Identity=26.8145161290323, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI221458488, Length=502, Percent_Identity=27.2908366533865, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI78706572, Length=138, Percent_Identity=36.231884057971, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=173, Percent_Identity=30.635838150289, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585711, Length=173, Percent_Identity=30.635838150289, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585713, Length=173, Percent_Identity=30.635838150289, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28574573, Length=167, Percent_Identity=31.1377245508982, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58738; Mature: 58738

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWMEIEKQRGIS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHH
VASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEAQTIKLLNV
HHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHH
CRLRDTPIVTFMNKYDREVRDSLELLDEVENILQIRCAPVTWPIGMGKNFKGVYHILNDE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCCCCHHEEEECCE
IYLFEAGDERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFNLDEFLAGE
EEEEECCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCC
LTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATVRMVEPDEAKFSGFIFKIQANMDPK
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCC
HRDRIAFLRVCSGKFERGMKMKHLRINREIAASSVVTFMSHDRELVEEAYAGDIIGIPNH
HHHHHHHHHHHCCHHHCCCEEEHEECCHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCC
GNIQIGDSFSEGEQLTFTGIPFFAPELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKP
CCEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECC
MSGADLILGAVGVLQFEVVTSRLANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCHHHHHHHHCCCC
GNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKL
CCEEEECCCCEEEECCCCEECCCCHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure
MRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWMEIEKQRGIS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHH
VASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEAQTIKLLNV
HHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHH
CRLRDTPIVTFMNKYDREVRDSLELLDEVENILQIRCAPVTWPIGMGKNFKGVYHILNDE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCCCCHHEEEECCE
IYLFEAGDERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFNLDEFLAGE
EEEEECCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCC
LTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATVRMVEPDEAKFSGFIFKIQANMDPK
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCC
HRDRIAFLRVCSGKFERGMKMKHLRINREIAASSVVTFMSHDRELVEEAYAGDIIGIPNH
HHHHHHHHHHHCCHHHCCCEEEHEECCHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCC
GNIQIGDSFSEGEQLTFTGIPFFAPELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKP
CCEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECC
MSGADLILGAVGVLQFEVVTSRLANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCHHHHHHHHCCCC
GNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKL
CCEEEECCCCEEEECCCCEECCCCHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA