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The map label for this gene is ppsA [H]

Identifier: 161869551

GI number: 161869551

Start: 589046

End: 591442

Strand: Reverse

Name: ppsA [H]

Synonym: NMCC_0566

Alternate gene names: 161869551

Gene position: 591442-589046 (Counterclockwise)

Preceding gene: 161869553

Following gene: 161869550

Centisome position: 27.47

GC content: 54.94

Gene sequence:

>2397_bases
TTGGACACACAAATGGCCGACAACTACGTAATTTGGTTTGAAAACCTGCGTATGACAGATGTTGAACGCGTGGGCGGTAA
AAACGCCTCGCTGGGCGAAATGATCAGTCAGCTGACCGAAAAAGGCGTTCGCGTCCCCGGCGGCTTTGCCACCACAGCCG
ATGCCTACCGCGCATTCCTCGCACACAACGGTCTGAGCGAACGCATTTCCGCCGCACTGGCAAAATTAGATGTCGAAGAC
GTTGCCGAACTGGCACGCGTCGGCAAAGAAATCCGCCAATGGATTTTGGACACGCCCTTCCCCGAACAGCTCGATGCCGA
AATCGAAGCGGCATGGAACAAAATGGTTGCCGATGCCGGCGGCGCGGACATTTCCGTTGCCGTACGCTCTTCCGCAACCG
CCGAAGACCTGCCCGACGCATCATTTGCCGGCCAACAGGAAACCTTCCTGAATATCAACGGCTTGGAAAACGTTAAAGAA
GCGATGCGCCATGTATTCGCCTCCCGGTACAACGACCGCGCCATTTCTTACCGCGTCCACAAAGGCTTCGAACACGACAT
CGTCGCCCTTTCCGCTGGCGTGCAGCGTATGGTGCGTTCCGACAGCGGCGCGTCCGGCGTGATGTTCACCCTCGATACCG
AATCCGGTTACGACCAAGTCGTCTTTGTCACTTCATCCTACGGTCTGGGCGAAAACGTCGTACAAGGCGCAGTCAATCCC
GACGAATTCTATGTGTTCAAACCCACGCTGAAAGCAGGCAAACCTGCCATCCTGCGCAAAACCATGGGCTCAAAACACAT
CAAAATGATTTTTACCGACAAAGCAGAAGCCGGCAAATCGGTAACCAACGTCGATGTCCCCGAAGAAGACCGCAACCGCT
TCTCCATTACCGACGAAGAAATCACCGAGTTGGCGCATTACGCACTGACCATCGAAAAACACTACGGCCGCCCGATGGAC
ATCGAATGGGGACGCGACGGCTTGGACGGCAAACTCTACATCCTGCAAGCCCGTCCCGAAACCGTCAAATCCCAGGAAGA
AGGCAGCCGCAACCTGCGCCGCTTTTCTATCAACGGCGAAAAAACCGTCTTGTGCGAAGGCCGCGCCATCGGTCAGAAAG
TCGGTCAGGGCAAGGTACGCCTGATTAAAGATGCTTCCGAGATGGATTCCGTCGAAGCCGGCGACGTACTGGTTACCGAC
ATGACCGACCCAGATTGGGAACCGGTGATGAAACGCGCTTCCGCCATCGTGACCAACAGGGGCGGACGTACCTGCCACGC
CGCCATCATCGCGCGCGAACTGGGTATTCCTGCCGTTGTCGGCTGCGGCAATGCAACCGAATTGCTGAAAAACGGTCAAG
AAGTTACCGTATCCTGTGCCGAAGGCGATACCGGCTTCATCTACTCCGGCCTGCTGGACGTACAAATTACCGATGTTGCT
TTGGACAACATGCCTAAAGCACCTGTAAAAGTCATGATGAACGTCGGCAATCCCGAACTCGCATTCAGCTTCGCCAACCT
GCCCAGCGAAGGCATCGGCTTGGCGCGTATGGAATTTATCATCAACCGCCAAATCGGTATCCACCCCAAAGCCTTGTTGG
AATTTGACAAACAAGACGACGAATTAAAAGCGGAAATTACCCGCCGTATCGCCGGTTACGCGTCCCCTGTCGACTTCTAC
GTCGATAAAATCGCCGAAGGCGTGGCGACATTGGCCGCATCGGTTTATCCGCGTAAAACCATCGTCCGTATGTCCGACTT
CAAATCCAACGAATACGCCAACCTGGTCGGCGGCAACGTATACGAACCGCATGAAGAAAACCCGATGTTGGGCTTCCGGG
GCGCGGCGCGTTATGTCGCCGACAACTTCAAAGACTGTTTCGCCTTGGAATGCAAAGCCTTGAAACGCGTCCGCGATGAA
ATGGGGTTGACCAACGTTGAAATCATGATTCCGTTCGTCCGCACTTTGGGCGAAGCCGAAGCCGTTGTCAAAGCCCTGAA
AGAAAACGGCTTGGAACGCGGCAAAAACGGCCTGCGCCTGATTATGATGTGCGAGCTGCCGAGCAACGCGGTATTGGCGG
AACAATTCCTGCAATACTTCGACGGCTTCTCCATCGGCTCGAACGACATGACCCAACTGACCCTCGGTCTCGACCGCGAC
AGCGGCTTGGTATCCGAATCGTTTGACGAACGCAACCCTGCCGTCAAAGTGATGCTGCACCTTGCCATCTCCGCCTGCCG
CAAGCAGAACAAATATGTCGGCATCTGCGGTCAAGGCCCGTCCGACCATCCGGACTTCGCCAAATGGCTGGTTGAGGAAG
GCATTGAAAGCGTTTCCCTGAACCCGGATACCGTCATCGAAACTTGGCTATATTTGGCGAATGAATTGAACAAATAA

Upstream 100 bases:

>100_bases
ACGGCATCATGATTTTAAAACGGATAAAACACATGACAGCAGAGGAACGAATCGCTTAAAATAAGCACGCGGATTTGTTT
CTTTTTTAACATATTTTGGA

Downstream 100 bases:

>100_bases
TCAATGCCCATACCCCCGAGCCTGAAAAGCGCGGGGGTATTTTTTTCCAAGCAGCTCCGCTCAGACGGCATTTCCTGCCG
ATGCCCCGTCCGCGATAATA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MDTQMADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGGFATTADAYRAFLAHNGLSERISAALAKLDVED
VAELARVGKEIRQWILDTPFPEQLDAEIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKE
AMRHVFASRYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVFVTSSYGLGENVVQGAVNP
DEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKSVTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMD
IEWGRDGLDGKLYILQARPETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDVLVTD
MTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQEVTVSCAEGDTGFIYSGLLDVQITDVA
LDNMPKAPVKVMMNVGNPELAFSFANLPSEGIGLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFY
VDKIAEGVATLAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFALECKALKRVRDE
MGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCELPSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRD
SGLVSESFDERNPAVKVMLHLAISACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK

Sequences:

>Translated_798_residues
MDTQMADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGGFATTADAYRAFLAHNGLSERISAALAKLDVED
VAELARVGKEIRQWILDTPFPEQLDAEIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKE
AMRHVFASRYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVFVTSSYGLGENVVQGAVNP
DEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKSVTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMD
IEWGRDGLDGKLYILQARPETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDVLVTD
MTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQEVTVSCAEGDTGFIYSGLLDVQITDVA
LDNMPKAPVKVMMNVGNPELAFSFANLPSEGIGLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFY
VDKIAEGVATLAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFALECKALKRVRDE
MGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCELPSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRD
SGLVSESFDERNPAVKVMLHLAISACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK
>Mature_798_residues
MDTQMADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGGFATTADAYRAFLAHNGLSERISAALAKLDVED
VAELARVGKEIRQWILDTPFPEQLDAEIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKE
AMRHVFASRYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVFVTSSYGLGENVVQGAVNP
DEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKSVTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMD
IEWGRDGLDGKLYILQARPETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDVLVTD
MTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQEVTVSCAEGDTGFIYSGLLDVQITDVA
LDNMPKAPVKVMMNVGNPELAFSFANLPSEGIGLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFY
VDKIAEGVATLAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFALECKALKRVRDE
MGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCELPSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRD
SGLVSESFDERNPAVKVMLHLAISACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=789, Percent_Identity=66.2864385297845, Blast_Score=1115, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=380, Percent_Identity=26.0526315789474, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788726, Length=380, Percent_Identity=27.1052631578947, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=31.5789473684211, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1789193, Length=409, Percent_Identity=24.6943765281174, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17564524, Length=237, Percent_Identity=27.0042194092827, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87728; Mature: 87728

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQMADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGGFATTADAYRAFL
CCCCCCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDAEIEAAWNKMVADAG
HHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
GADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRHVFASRYNDRAISYRVH
CCEEEEEEECCCCHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEC
KGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVFVTSSYGLGENVVQGAVNP
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCC
DEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKSVTNVDVPEEDRNRFSITDEE
CCEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHH
ITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQARPETVKSQEEGSRNLRRFSINGE
HHHHHHHHEEEHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCHHHCCCCCEEEEECCC
KTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDVLVTDMTDPDWEPVMKRASAIVTNR
EEEEECCHHHHHHCCCCCEEEEECHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
GGRTCHAAIIARELGIPAVVGCGNATELLKNGQEVTVSCAEGDTGFIYSGLLDVQITDVA
CCCCHHHHHHHHHCCCCEEEECCCHHHHHHCCCEEEEEECCCCCCEEEECEEEEEEEEEE
LDNMPKAPVKVMMNVGNPELAFSFANLPSEGIGLARMEFIINRQIGIHPKALLEFDKQDD
CCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCH
ELKAEITRRIAGYASPVDFYVDKIAEGVATLAASVYPRKTIVRMSDFKSNEYANLVGGNV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHCCCCC
YEPHEENPMLGFRGAARYVADNFKDCFALECKALKRVRDEMGLTNVEIMIPFVRTLGEAE
CCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHH
AVVKALKENGLERGKNGLRLIMMCELPSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRD
HHHHHHHHCCHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
SGLVSESFDERNPAVKVMLHLAISACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSL
CCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCC
NPDTVIETWLYLANELNK
CCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTQMADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGGFATTADAYRAFL
CCCCCCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDAEIEAAWNKMVADAG
HHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
GADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRHVFASRYNDRAISYRVH
CCEEEEEEECCCCHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEC
KGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVFVTSSYGLGENVVQGAVNP
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCC
DEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKSVTNVDVPEEDRNRFSITDEE
CCEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHH
ITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQARPETVKSQEEGSRNLRRFSINGE
HHHHHHHHEEEHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCHHHCCCCCEEEEECCC
KTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDVLVTDMTDPDWEPVMKRASAIVTNR
EEEEECCHHHHHHCCCCCEEEEECHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
GGRTCHAAIIARELGIPAVVGCGNATELLKNGQEVTVSCAEGDTGFIYSGLLDVQITDVA
CCCCHHHHHHHHHCCCCEEEECCCHHHHHHCCCEEEEEECCCCCCEEEECEEEEEEEEEE
LDNMPKAPVKVMMNVGNPELAFSFANLPSEGIGLARMEFIINRQIGIHPKALLEFDKQDD
CCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCH
ELKAEITRRIAGYASPVDFYVDKIAEGVATLAASVYPRKTIVRMSDFKSNEYANLVGGNV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHCCCCC
YEPHEENPMLGFRGAARYVADNFKDCFALECKALKRVRDEMGLTNVEIMIPFVRTLGEAE
CCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHH
AVVKALKENGLERGKNGLRLIMMCELPSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRD
HHHHHHHHCCHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
SGLVSESFDERNPAVKVMLHLAISACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSL
CCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCC
NPDTVIETWLYLANELNK
CCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]