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The map label for this gene is trmD [H]

Identifier: 161869522

GI number: 161869522

Start: 560767

End: 561516

Strand: Reverse

Name: trmD [H]

Synonym: NMCC_0536

Alternate gene names: 161869522

Gene position: 561516-560767 (Counterclockwise)

Preceding gene: 161869523

Following gene: 161869521

Centisome position: 26.08

GC content: 53.07

Gene sequence:

>750_bases
ATGCTTATCCAGGCAGTTACCATTTTCCCCGAAATGTTCGACAGCATTACCCGCTACGGCGTAACGGGACGCGCGAACAG
ACAGGGAATCTGGCAGTTTGAAGCAGTCAATCCCCGAAAGTTTGCCGACAACAGATTGGGCTACATCGACGACCGCCCGT
TCGGCGGCGGTCCGGGAATGATTATGATGGCTCCGCCGCTTCATGCGGCAATAGAACACGCCAAAGCACAATCTTCCCAA
ACCGCAAAAGTCATCTACCTCAGCCCCCAAGGAAAACCGCTGACACACCAAAAAGCGGCAGAACTGGCAGAACTTCCGCA
TCTGATTCTGCTGTGCGGACGCTATGAGGGAATAGACGAAAGACTGCTGCAAACCAGCGTCGATGAAGAAATCAGCATCG
GAGACTTCGTTGTTTCCGGCGGAGAGCTTCCCGCCATGATGCTGATGGATGCGGTATTGAGGCTCGTACCCGGCGTATTG
GGCGATATGCAGTCTGCCGAACAAGATTCGTTCTCAAGCGGCATTTTGGACTGCCCCCACTACACCAAACCCTTAGAATT
TCAAGGCATGGCTGTTCCGGAAGTATTACGTTCCGGCAATCATGGCTTGATAGCGGAATGGCGGTTGGAACAATCGCTGC
GCCGCACCTTGGAGCGCAGACCCGATCTTTTGGAAAAGCGCGTTTTAATCCCAAAGGAATCCCGCCTCTTAGAAACCATC
CGGCAAGAGCAACGGGAAATCCAATCATAA

Upstream 100 bases:

>100_bases
GAGAACACGGGCAGATTCTGATTCCGTTCGTTTCCCAATATATCGAAACCGTCGATACCGGCAGCAAGACCATTACTGCC
GACTGGGGTTTGGACTACTG

Downstream 100 bases:

>100_bases
TTTAGGAAAAAAACAATGAACCTGATTCAACAGCTCGAGCAAGAAGAAATTGCCCGTCTGAACAAAGACATCCCCGAATT
CGCACCGGGCGACACCGTAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADNRLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQ
TAKVIYLSPQGKPLTHQKAAELAELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLERRPDLLEKRVLIPKESRLLETI
RQEQREIQS

Sequences:

>Translated_249_residues
MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADNRLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQ
TAKVIYLSPQGKPLTHQKAAELAELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLERRPDLLEKRVLIPKESRLLETI
RQEQREIQS
>Mature_249_residues
MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADNRLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQ
TAKVIYLSPQGKPLTHQKAAELAELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLERRPDLLEKRVLIPKESRLLETI
RQEQREIQS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=248, Percent_Identity=56.0483870967742, Blast_Score=270, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27874; Mature: 27874

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADNRLGYIDDRPFGGGPGM
CEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHCCCCCCCCCCCCCCCCCCE
IMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAELAELPHLILLCGRYEGIDE
EEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHH
RLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVLGDMQSAEQDSFSSGILDCPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLERRPDLLEKRVLIPKESRLLETI
CCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHH
RQEQREIQS
HHHHHHHCC
>Mature Secondary Structure
MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADNRLGYIDDRPFGGGPGM
CEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHCCCCCCCCCCCCCCCCCCE
IMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAELAELPHLILLCGRYEGIDE
EEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHH
RLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVLGDMQSAEQDSFSSGILDCPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLERRPDLLEKRVLIPKESRLLETI
CCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHH
RQEQREIQS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA