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The map label for this gene is priA [H]

Identifier: 161869481

GI number: 161869481

Start: 514003

End: 516204

Strand: Direct

Name: priA [H]

Synonym: NMCC_0493

Alternate gene names: 161869481

Gene position: 514003-516204 (Clockwise)

Preceding gene: 161869479

Following gene: 161869486

Centisome position: 23.87

GC content: 60.08

Gene sequence:

>2202_bases
TTGCCTGCCTGTATGATCTACCACCGCATCGCCGTAAACGTGCCGCTTTCAGACGGCCTTTTGACTTATTCCCATTCCGA
TCCGCTTCCTCCGGGAACGCGGGTGCTTGTGCCTTTCCGCAATAAAACCGTGGTCGGGATGGTGTGGGAAACGGATATTG
CGCCGGATATGGATACGGCGCGGATTTTGAGTATTCAGACGACCTTTTTAGACGAGCCGCCGTTGCCTGAAAGCTGGCGT
GATTTGCTCTCGTTTACGTCGCGTTATTACCACTATCCGACGGGGCAGGCGGTGTTTGCCGCGCTGCCGCAGGGTTTGAA
GGAAACGCGCGCGGTGGAAATGCCGCAGCCGCCGTTGTTTTACGCGCTGAACGAAGCGGGCAGGGCGCAAACGCCGCCGC
TGGCGCGGTTCAACAAAAAAGCGGCTTTGTGGGACGCACTGCTGTCGGGCGGGATGACGATGGCGGCGTTGAAGCAGGTA
AACGCGCAGGCGGCGAAATTGATTGAGGATTGGGCTGAACAAGGCTGGATTGAAACGACGGAAGCGGCGAAGCCCGTTTT
AAGGTCGTGTAATGGGCAGGCTTCGCACTCTGAATTTGTATTAAATGCTGACCAGCAGAAGGCTTCCGATGCCATTCAGA
CGGCATTGGGACGCTTCCAGCCGTTTCTGCTGTACGGCATCACCGGCAGCGGCAAGACCGAGGTGTATTTTGATGCGATG
GCGAAAGTGTTGGCTCAGGGGCGGCAGGTGTTGTTTCTGTTGCCCGAAATCAACCTCACGCCGCAGCTTTTGAAGCGGGT
GGAAAACCGTTTTGCCGACGTGCCGACCGCCGTGTTGCACAGTCAGATGGCGGCAGGCAAGCGCACGCAGGATTATCTGC
GCGCGATGTTGGGGCAGGCGAAGCTGGTGATCGGCACGCGGTTGGCGGTGTTCACGCCGATGGATGATGTCGGGCTGATT
GTGGTCGATGAGGAACACGACGGCTCGTTCAAACAGGACAACGAATTGCGCTACCACGCCCGCGATTTGGCGGTGTGGCG
GGCGAAGCAGGGCGGCTGCCCGATCATATTGGGCAGTGCCACCCCCAGCTTGGAGAGCTGGCACAAGGCGCAAAGCGGCG
CGTACCGCCTGCTGCAACTGACCGAACGCGCCCATACCGCCGCGCAACTGCCGCAAGTGGACATCCTCAACGTAGGCCGT
CTGAAACTTGACAACGGCTTCTCGCCGCAAGCCTTGCAGCTTTTGAAACAGAACTTTGAAGCAGGTGGCATGTCGTTGGT
GTACCTCAACCGTCGCGGCTTCGCGCCCGCGCTGTTTTGCGGCGACTGCGGTTATACCTTCGGCTGCCCGAACTGCTCCG
CCAAAATGGTGCTGCACCAACGCGTCCGCCAACTGCGCTGCCACCACTGCGACCACCGCGAACCCATACCGTTCAAATGC
CCCGACTGCGGCAATCAAGACCTGACCGCCGTCGGACACGGCACGCAGCGCGTCGAAGAAACCCTGCGCGCTTTCCTGCC
CAAGGCCGCCGTCGTCCGCGTGGACAGGGACAGCACCGCGCACAAAAACGACTGGGCGGATTTGTACCGCCGCATCGCCG
ACAACGAAATCGATATTCTGGTCGGCACGCAGATGCTCGCCAAAGGTCATGATTTCGCGCGGCTCAACCTCGTTATCGTA
TTGAACGCCGACGGCAGCCTGTACAGCGCGGATTTCCGCGCGCCGGAAAGGCTGTTTGCCGAGCTGATGCAGGTGTCCGG
CAGGGCGGGGCGCGCCGACAAGCCCGGCAAGGTGCTGATACAGACCCAACTGCCCGAACATCCCGTCTTCGCCGCCGTCA
AAGCGCAGGACTACGTCGTGTTTGCCGAAAACGAATTGAATGAGCGGCAAATGTTCGCCATGCCGCCCTTCGGTTTTCAG
ACGGCCGTCCGCGCGGACGCGCCGCGTGTTGCCGATGCGATGGAATTTCTCAACGCCGCCAAAGAAACCCTCGCCCCGCT
TTTGCCCGAAAGCGTTTCACAGTTCGGCGCCGCCCCGATGCTGATGGTGCGCCTCGCCGAACGCGAACGCGCGCAAATCT
TCCTCGAATCAACATCCCGACAGGATTTGCACCGCGCCGTGAGTTTGTGGGTGCAGGTGTTGCAGCAAAACAGGGACGGC
AAAATCCGATGGTCGGTGGATGTCGATCCGCATGAGGCTTGA

Upstream 100 bases:

>100_bases
AGCGGAATTGTATCAAATCTCTGTTGCGCCTGCATTGCGCCTAGGCTCAATTTATCGTCTGAAAATAGCTTCCGCCTGTT
AAAATACGCAAAAAAATGAT

Downstream 100 bases:

>100_bases
TTATTGGCAATCCGATGCCGTCTGAAAACCGTTTCAGACGGCATTTTTATTCCGGATCGTCTGTAAACGCGTTCGCCCGA
AAGATCGGTACGAATGTCAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MPACMIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMVWETDIAPDMDTARILSIQTTFLDEPPLPESWR
DLLSFTSRYYHYPTGQAVFAALPQGLKETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQV
NAQAAKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPFLLYGITGSGKTEVYFDAM
AKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLHSQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLI
VVDEEHDGSFKQDNELRYHARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDILNVGR
LKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSAKMVLHQRVRQLRCHHCDHREPIPFKC
PDCGNQDLTAVGHGTQRVEETLRAFLPKAAVVRVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIV
LNADGSLYSADFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNERQMFAMPPFGFQ
TAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLAERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDG
KIRWSVDVDPHEA

Sequences:

>Translated_733_residues
MPACMIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMVWETDIAPDMDTARILSIQTTFLDEPPLPESWR
DLLSFTSRYYHYPTGQAVFAALPQGLKETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQV
NAQAAKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPFLLYGITGSGKTEVYFDAM
AKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLHSQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLI
VVDEEHDGSFKQDNELRYHARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDILNVGR
LKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSAKMVLHQRVRQLRCHHCDHREPIPFKC
PDCGNQDLTAVGHGTQRVEETLRAFLPKAAVVRVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIV
LNADGSLYSADFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNERQMFAMPPFGFQ
TAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLAERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDG
KIRWSVDVDPHEA
>Mature_732_residues
PACMIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMVWETDIAPDMDTARILSIQTTFLDEPPLPESWRD
LLSFTSRYYHYPTGQAVFAALPQGLKETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVN
AQAAKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPFLLYGITGSGKTEVYFDAMA
KVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLHSQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIV
VDEEHDGSFKQDNELRYHARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDILNVGRL
KLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSAKMVLHQRVRQLRCHHCDHREPIPFKCP
DCGNQDLTAVGHGTQRVEETLRAFLPKAAVVRVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVL
NADGSLYSADFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNERQMFAMPPFGFQT
AVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLAERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGK
IRWSVDVDPHEA

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=708, Percent_Identity=38.8418079096045, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81452; Mature: 81321

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPACMIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMVWETDIAPDMDTA
CCCEEEEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCC
RILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGLKETRAVEMPQPPLF
EEEEEEEEECCCCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCEE
YALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVNAQAAKLIEDWAEQGWIETT
EEECCCCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPFLLYGITGSGKTEVYFDAM
HHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
AKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLHSQMAAGKRTQDYLRAMLGQA
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCC
KLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRYHARDLAVWRAKQGGCPIILGSA
EEEEECEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCCEEECCC
TPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDILNVGRLKLDNGFSPQALQLLKQNFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCHHHHHHHHHCCC
AGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSAKMVLHQRVRQLRCHHCDHREPIPFKC
CCCEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PDCGNQDLTAVGHGTQRVEETLRAFLPKAAVVRVDRDSTAHKNDWADLYRRIADNEIDIL
CCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEE
VGTQMLAKGHDFARLNLVIVLNADGSLYSADFRAPERLFAELMQVSGRAGRADKPGKVLI
ECHHHHHCCCCEEEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
QTQLPEHPVFAAVKAQDYVVFAENELNERQMFAMPPFGFQTAVRADAPRVADAMEFLNAA
EECCCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCCCHHHHHCCCCHHHHHHHHHHHH
KETLAPLLPESVSQFGAAPMLMVRLAERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDG
HHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCC
KIRWSVDVDPHEA
CEEEEECCCCCCC
>Mature Secondary Structure 
PACMIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMVWETDIAPDMDTA
CCEEEEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCC
RILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGLKETRAVEMPQPPLF
EEEEEEEEECCCCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCEE
YALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVNAQAAKLIEDWAEQGWIETT
EEECCCCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPFLLYGITGSGKTEVYFDAM
HHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
AKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLHSQMAAGKRTQDYLRAMLGQA
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCC
KLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRYHARDLAVWRAKQGGCPIILGSA
EEEEECEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCCEEECCC
TPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDILNVGRLKLDNGFSPQALQLLKQNFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCHHHHHHHHHCCC
AGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSAKMVLHQRVRQLRCHHCDHREPIPFKC
CCCEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PDCGNQDLTAVGHGTQRVEETLRAFLPKAAVVRVDRDSTAHKNDWADLYRRIADNEIDIL
CCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEE
VGTQMLAKGHDFARLNLVIVLNADGSLYSADFRAPERLFAELMQVSGRAGRADKPGKVLI
ECHHHHHCCCCEEEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
QTQLPEHPVFAAVKAQDYVVFAENELNERQMFAMPPFGFQTAVRADAPRVADAMEFLNAA
EECCCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCCCHHHHHCCCCHHHHHHHHHHHH
KETLAPLLPESVSQFGAAPMLMVRLAERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDG
HHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCC
KIRWSVDVDPHEA
CEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]