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The map label for this gene is carA [H]

Identifier: 161869367

GI number: 161869367

Start: 373603

End: 374736

Strand: Reverse

Name: carA [H]

Synonym: NMCC_0373

Alternate gene names: 161869367

Gene position: 374736-373603 (Counterclockwise)

Preceding gene: 161869369

Following gene: 161869366

Centisome position: 17.4

GC content: 57.05

Gene sequence:

>1134_bases
ATGAGCACCCCCGCCCTCCTCGTCCTCGCTGACGGCAGCGTATTTCACGGCACATCAATCGGTTATGAAGGTTCGACTTC
CGGCGAAGTCGTGTTCAATACTTCGATGACTGGCTATCAGGAAATCCTGACCGACCCGTCCTACTGCAAACAAATCGTTA
CCCTCACCTACCCACACATCGGCAACACCGGCACCAACGCCGAAGATGAAGAAAGCCGCAGCGTTTATGCCGCCGGCCTG
ATTATCCGCGACCTGCCGCTCTTGCACAGCAACTTCCGCGCCTCCGAAAGCCTGCACGACTATCTGGTACGCAACAAAAC
CGTCGCCATCGCCGACATCGATACCCGCCGCCTGACCACGCTGTTGCGCGAAAAAGGCGCGCAAGGCGGCGCGATTCTGA
CCGGTGCGGATGCCACAATCGAAAAAGCGCAAGAACTCATCGCCGCGTTCGGCAGTATGGTCGGCAAAGACTTGGCAAAA
GAAGTTTCCTGCACGGAAACTTACGAATGGACGGAAGGCGAATGGGAATTGGGCAAGGGTTTCGTTACCCCTGACGAACA
GCCTTACCACGTCGTCGCCTACGATTTCGGCGTGAAAACCAACATCCTGCGTATGCTCGCCTCGCGCGGCTGCCGCCTGA
CCGTCGTCCCCGCCCAAACGAGCGCACAAGACGTGTTGGCACTCAATCCCGACGGCGTGTTCCTGTCCAACGGCCCCGGC
GACCCCGAGCCTTGCACCTACGCCATCGAAGCCGTGCAAAAACTGATGGAAAGCGGCAAACCGATTTTCGGCATCTGCTT
GGGACACCAGCTCATCAGCCTCGCTATCGGCGCGAAAACCCTGAAAATGCGCTTCAGCCACCACGGTGCGAACCACCCTG
TGCAAGATTTGGACAGCGGCAAAGTCGTCATCACCAGCCAAAACCACGGTTTTGCCGTTGATGCCGACACCCTGCCCGCT
AACGCACGCATTACCCACAAATCCTTGTTTGACAACACTTTGCAAGGCATCGAGCTGACCGACAAACCTGTGTTCTGCTT
CCAAGGCCACCCCGAAGCCAGCCCCGGTCCGCAAGATGTCGGCTATTTGTTTGACAAATTCATTGGCAATATGAAAGCGG
CAAAACGGGCATAA

Upstream 100 bases:

>100_bases
GCGTGTTTTTTTTTGACCGGATTTTTCCGACCGGATGCCCCCTGCCGAAGTCCCTTCAGACGGCATTGTCAAGAATTTTA
TTAAAAACAGGATTCCCATT

Downstream 100 bases:

>100_bases
TGGCTTTCAGACGGCACTGCCGCCGCCTGAAAAAGAAAGCGGGAAATGAAGATTAGCGCACTCGACCATCTGGTACTGAC
CGTTGCCGACATTGACCGAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILTDPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGL
IIRDLPLLHSNFRASESLHDYLVRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPAQTSAQDVLALNPDGVFLSNGPG
DPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAIGAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPA
NARITHKSLFDNTLQGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA

Sequences:

>Translated_377_residues
MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILTDPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGL
IIRDLPLLHSNFRASESLHDYLVRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPAQTSAQDVLALNPDGVFLSNGPG
DPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAIGAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPA
NARITHKSLFDNTLQGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA
>Mature_376_residues
STPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILTDPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLI
IRDLPLLHSNFRASESLHDYLVRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAKE
VSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPAQTSAQDVLALNPDGVFLSNGPGD
PEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAIGAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPAN
ARITHKSLFDNTLQGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=37.7952755905512, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI21361331, Length=386, Percent_Identity=37.8238341968912, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI169790915, Length=386, Percent_Identity=37.8238341968912, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1786215, Length=371, Percent_Identity=67.1159029649596, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI193204318, Length=384, Percent_Identity=39.0625, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=38.5786802030457, Blast_Score=238, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=386, Percent_Identity=37.3056994818653, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=38.2051282051282, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=38.2051282051282, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40663; Mature: 40532

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILTDPSYCKQIVTLTYPHI
CCCCEEEEEECCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHEECCCC
GNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYLVRNKTVAIADIDTRRLTT
CCCCCCCCCCHHCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHH
LLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAKEVSCTETYEWTEGEWELGKG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCC
FVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPAQTSAQDVLALNPDGVFLSNGPG
CCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCCCC
DPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAIGAKTLKMRFSHHGANHPVQDLDSG
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCHHHEEEEHHHCCCCCCHHHCCCC
KVVITSQNHGFAVDADTLPANARITHKSLFDNTLQGIELTDKPVFCFQGHPEASPGPQDV
EEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHCCCEECCCCEEEECCCCCCCCCHHHH
GYLFDKFIGNMKAAKRA
HHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
STPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILTDPSYCKQIVTLTYPHI
CCCEEEEEECCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHEECCCC
GNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYLVRNKTVAIADIDTRRLTT
CCCCCCCCCCHHCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHH
LLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAKEVSCTETYEWTEGEWELGKG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCC
FVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPAQTSAQDVLALNPDGVFLSNGPG
CCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCCCC
DPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAIGAKTLKMRFSHHGANHPVQDLDSG
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCHHHEEEEHHHCCCCCCHHHCCCC
KVVITSQNHGFAVDADTLPANARITHKSLFDNTLQGIELTDKPVFCFQGHPEASPGPQDV
EEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHCCCEECCCCEEEECCCCCCCCCHHHH
GYLFDKFIGNMKAAKRA
HHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7773412 [H]