Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 161869021

GI number: 161869021

Start: 918

End: 2312

Strand: Reverse

Name: gltX [H]

Synonym: NMCC_0003

Alternate gene names: 161869021

Gene position: 2312-918 (Counterclockwise)

Preceding gene: 161869022

Following gene: 161869020

Centisome position: 0.11

GC content: 55.2

Gene sequence:

>1395_bases
ATGACCGTCAAAACCCGTTTCGCCCCCAGCCCAACCGGCTACCTGCACATCGGCGGCGTACGCACCGCCCTGTTTTCTTG
GGCGTTTGCCCGCCATCATAAAGGCGAATTCTTATTGCGCATCGAAGACACCGACTTGGCGCGCTCTACTGCCGAATCCG
TCAACATCATCCTCGACGGCATGAAATGGGTCGGACTCGATTACGACAACGCCGGCAACGTCGTGTACCAAACCCGCCGT
TTCGACCGCTATAAAGAAGTCATCGCCGAACTTTTAGAAAAAGGCGATGCCTACTACTGCTATTGCAGCAAAGAAGAGTT
GGAAGCCATGCGCGAGAAAGCCGAAAAAGAAGGCACGGCGACTTACGACCGCCGCTGGCGGCCGGAAGCAGGCAAAACCC
TGCCCGAAATCCCTGCCGGCGTGCAACCCGTCGTCCGCTTCAAAACGCCTTTGGATGGCGTAACCAAGTGGACGGACTTG
GTCAAAGGCGAAATCTCCATCCCCAACGAAGCACTCGACGACCTGATTATCGCCCGCGCCGACGGCACGCCGACCTACAA
CTTCTGCGTTGTCGTGGACGACTACGACATGGGCGTTACCCACGTTATCCGGGGCGACGACCATGTGAACAACACCCCGA
AACAAATCAACATCTTAAAAGCCATCGGCGCTACCCTGCCTGAATACGGCCACCTGCCGATGATTCTTAACGAACAAGGC
AAAAAAATCTCCAAACGCAGCGGCGATACCGTTGCCATTACCGATTTCGGCGCGATGGGCATCCTGCCCGAAGCCATGCT
CAACTATCTGGCACGTTTGGGCTGGGCGCACGGCGACGATGAGTTCTTCACGATGGAACAATTTATCGAATGGTTTGATT
TGAAAGACGTTTCTCCATCTCCAAGCCGCATGGATTTGAAGAAACTCTACTGGATCAACGGCGAACACATCAAAATCACG
CCCAACGGCAAACTCGCCGAACTCGTCAAACCGCGTCTCGCTCTGCGCGATATCCATGAAACTGAAAAACCTGCATTGGA
AGACGTGTTGGCACTGGTCAAAGACCGCGCCCAAGACCTGAACACACTTGCCGACGAGTGCCTCTACTTCTACGTCAAAC
AAACGCCTACTGAAGCGGACGTGCAGAAACACTGGGACGATGAAGCCGCCGCCCGTATGCTGCGCTTCGCCGAACGCCTC
GAAGGGCTGGAAGACTGGAATACCGAAGCCATCCACGACCTCTTCAAACCCTTCTGCGACGAAGAAGGCATTAAAATGGG
CAAACTCGGCATGCCCCTGCGCCTTGCCGTCTGCGGTACGGCGAAAACCCCCAGCGTCGATGCTGTGTTGGCATTAATCG
GCAAAGAGGAAGTGTTGAAACGCATCCGCGCTTAA

Upstream 100 bases:

>100_bases
CGTCTGAAACTTATCCGCAGGCAGGGTTCGGACGGCATTTTTGATGCCCAAGCACGGTAAAAACAGTAAAATAAGCCGTT
TTAAAGATTTTGGAGCCATC

Downstream 100 bases:

>100_bases
GTTATCCACAGGCATGGGCGATGCCGTCTGATGTCGGAATACGGCTTCAGACGGCATTCAAACGGAAGTTGAACATGAAA
AAAATATTGTATTTTCTGAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDTDLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRR
FDRYKEVIAELLEKGDAYYCYCSKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINILKAIGATLPEYGHLPMILNEQG
KKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWAHGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKIT
PNGKLAELVKPRLALRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAARMLRFAERL
EGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVLALIGKEEVLKRIRA

Sequences:

>Translated_464_residues
MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDTDLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRR
FDRYKEVIAELLEKGDAYYCYCSKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINILKAIGATLPEYGHLPMILNEQG
KKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWAHGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKIT
PNGKLAELVKPRLALRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAARMLRFAERL
EGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVLALIGKEEVLKRIRA
>Mature_463_residues
TVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDTDLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRF
DRYKEVIAELLEKGDAYYCYCSKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDLV
KGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINILKAIGATLPEYGHLPMILNEQGK
KISKRSGDTVAITDFGAMGILPEAMLNYLARLGWAHGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITP
NGKLAELVKPRLALRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAARMLRFAERLE
GLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVLALIGKEEVLKRIRA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=30.9090909090909, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI4826960, Length=308, Percent_Identity=24.6753246753247, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=47.6293103448276, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI226510925, Length=286, Percent_Identity=36.013986013986, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1786895, Length=210, Percent_Identity=27.6190476190476, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=498, Percent_Identity=28.9156626506024, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=486, Percent_Identity=32.0987654320988, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=217, Percent_Identity=28.110599078341, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=216, Percent_Identity=27.3148148148148, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=31.3131313131313, Blast_Score=228, Evalue=1e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52422; Mature: 52290

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDTDLARSTAESVNIILDG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHEEEEC
MKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYCSKEELEAMREKAEKEGTA
CEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCC
TYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDLVKGEISIPNEALDDLIIARA
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCHHHHHHEEEEEC
DGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINILKAIGATLPEYGHLPMILNEQG
CCCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCC
KKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWAHGDDEFFTMEQFIEWFDLKDVSPS
CHHHHCCCCEEEEECCCCHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
PSRMDLKKLYWINGEHIKITPNGKLAELVKPRLALRDIHETEKPALEDVLALVKDRAQDL
CCCCCHHHHEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NTLADECLYFYVKQTPTEADVQKHWDDEAAARMLRFAERLEGLEDWNTEAIHDLFKPFCD
HHHHHHHHEEHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCC
EEGIKMGKLGMPLRLAVCGTAKTPSVDAVLALIGKEEVLKRIRA
CCCCEECCCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
TVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDTDLARSTAESVNIILDG
CCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHEEEEC
MKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYCSKEELEAMREKAEKEGTA
CEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCC
TYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDLVKGEISIPNEALDDLIIARA
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCHHHHHHEEEEEC
DGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINILKAIGATLPEYGHLPMILNEQG
CCCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCC
KKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWAHGDDEFFTMEQFIEWFDLKDVSPS
CHHHHCCCCEEEEECCCCHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
PSRMDLKKLYWINGEHIKITPNGKLAELVKPRLALRDIHETEKPALEDVLALVKDRAQDL
CCCCCHHHHEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NTLADECLYFYVKQTPTEADVQKHWDDEAAARMLRFAERLEGLEDWNTEAIHDLFKPFCD
HHHHHHHHEEHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCC
EEGIKMGKLGMPLRLAVCGTAKTPSVDAVLALIGKEEVLKRIRA
CCCCEECCCCCCEEEEEECCCCCCCHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA