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The map label for this gene is leuA [H]

Identifier: 161777625

GI number: 161777625

Start: 1727222

End: 1728892

Strand: Reverse

Name: leuA [H]

Synonym: Rru_A1461

Alternate gene names: 161777625

Gene position: 1728892-1727222 (Counterclockwise)

Preceding gene: 83592799

Following gene: 83592795

Centisome position: 39.72

GC content: 59.9

Gene sequence:

>1671_bases
ATGTTGACCAATCCTGAAATCAAATATCGCCCTTTCGTTTCGCCCATTGAGCTCGACGATCGCAAATGGCCGTCCAAGCG
GCTCACGAAAGCCCCGCGCTGGCTGTCTACCGATCTGCGAGATGGCAATCAGGCGCTCTCCGATCCGATGGATGTCGAGC
GCAAGATGCGTTTTTACGCCATGCTGCTCGCCATCGGTTTCAAAGAAATCGAGATTGCGTTTCCGTCGGCTTCCCAGACC
GAATTTGACTTCGTGCGCGCCCTGATTGGGGGAAACCGGATACCCGATGACGTGACGATTCAGGTCCTCACCCCATCGCG
CCAAGACCTGATCGCTCGCACCTTCGAATCTCTCGAGGGCGCGCGACAGGTAATCGTTCATCTCTATAACGCCACGGCTC
CGCTGTTTCGCCGTGTCGTCTTCGGCATGGAGCGCCATGAGATCGTTGATCTTGCCATCACCGGTGTCAGCGCGATGCTG
GAGGAAAGCCTGAGGCGGCCCGAGACCGATTGGACCTTCGAATACTCGCCGGAAACCTTCTGCTTCACCGAGCCCGATTT
CGCGCTGGAGATTTGCGAGCGCGTGCTTGATGTCTGGCAGCCGACGCCCGAGCGCCCGGCGATCCTCAACCTGCCGGCGA
CGGTCGAAGTGGCGACGCCCAACGTCTATGCCGACCAGATCGAATGGTTTTGCCGTAATATATCGCGTCGCGAGGCGGTG
GTGATCAGCATTCATCCGCACAACGACCGGGGAACCGCCGTCGCCGCGGCTGAACAGGCCTTGCTGGCTGGGGCGGATCG
TGTCGAAGGCTGCCTGTTTGGCAATGGTGAGCGGACGGGCAATGTCGATCTGGTGACCTTGGCCTTGAACTTCTACTCGC
AAGGGATCGATCCGAAGCTCTCTTTTCCGGCGATCCGCGACGTGGTGACGACCGTTGAGCATTGCAACGGACTGCCCGTC
CATCCACGTCACCCCTATGCGGGCGAGCTGGTGCACACGGCCTTCTCCGGCTCGCATCAAGATGCGATCCGCAAAGGGTT
CACCGCTCATCGACAGCGCAACGACGGCATTTGGGAGATGCCCTATTTGCCCGTCGATCCGGCCGACATCGGCGAGACAT
ACGAAGCGGTGATCCGCGTCAACAGCCAGTCCGGCAAGGGGGGCGTCGCCTGGGTGATCGAACAAGACAAGGGCCTGAAA
CTGCCACGATCGCTTCAGAGGGATCTCAGCCGTCGCGTTCAGGAATTCTCCGAGAAAAGCGGCAAGGAGATCACCGCCGC
GATGATCTGGTCGATCTTTCAGCAGGCTTACCACCTGACGGGCCCGCAGGAGTTCGAACTCATCAGCTATCAGGATGGGA
ACTCCCGCAAAGCCGGGAACCACCGCGTCTTCGCCGGGCGCATCCGTCATAAGGGTCGCGAAATCGCCCTCTCCGGTCGT
GGCAACGGCCTGATTTCCAGCGTTGTCGCCGCATTGGAAGACGGCTGTGGCGTCGCGCTGGATGTGGTCGACTTTCACGA
ACATGCCTTGAAGCGTGGTTCGGATTCCCAGGCGGTTGCCTATCTCGAATGTCGAACCCAAGACGATCGCCCGATTTTCG
GAGTCGGTATCGACGATGATATCGCAACGGCATCGGTCAAGGCCGTGCTCAGCGCCGCCAGCAACGGCTGA

Upstream 100 bases:

>100_bases
GCCGCGACTTACCGGCGGTCGTCGGGCCTGACGGCTTCCCGGCGACCGTAGTCGGCCGTGTCAAATCGGACACACACATT
GAATGGCAACGGATCACCCC

Downstream 100 bases:

>100_bases
CCTCGTTTTGCGGCCGGGCTTTCAAGGCCCGGCCGTCCCTCTAACGCTCAACACCAGCATCCCCGAAAGCACCCGCAACC
GCCCCCAGGTCATAAAACCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MLTNPEIKYRPFVSPIELDDRKWPSKRLTKAPRWLSTDLRDGNQALSDPMDVERKMRFYAMLLAIGFKEIEIAFPSASQT
EFDFVRALIGGNRIPDDVTIQVLTPSRQDLIARTFESLEGARQVIVHLYNATAPLFRRVVFGMERHEIVDLAITGVSAML
EESLRRPETDWTFEYSPETFCFTEPDFALEICERVLDVWQPTPERPAILNLPATVEVATPNVYADQIEWFCRNISRREAV
VISIHPHNDRGTAVAAAEQALLAGADRVEGCLFGNGERTGNVDLVTLALNFYSQGIDPKLSFPAIRDVVTTVEHCNGLPV
HPRHPYAGELVHTAFSGSHQDAIRKGFTAHRQRNDGIWEMPYLPVDPADIGETYEAVIRVNSQSGKGGVAWVIEQDKGLK
LPRSLQRDLSRRVQEFSEKSGKEITAAMIWSIFQQAYHLTGPQEFELISYQDGNSRKAGNHRVFAGRIRHKGREIALSGR
GNGLISSVVAALEDGCGVALDVVDFHEHALKRGSDSQAVAYLECRTQDDRPIFGVGIDDDIATASVKAVLSAASNG

Sequences:

>Translated_556_residues
MLTNPEIKYRPFVSPIELDDRKWPSKRLTKAPRWLSTDLRDGNQALSDPMDVERKMRFYAMLLAIGFKEIEIAFPSASQT
EFDFVRALIGGNRIPDDVTIQVLTPSRQDLIARTFESLEGARQVIVHLYNATAPLFRRVVFGMERHEIVDLAITGVSAML
EESLRRPETDWTFEYSPETFCFTEPDFALEICERVLDVWQPTPERPAILNLPATVEVATPNVYADQIEWFCRNISRREAV
VISIHPHNDRGTAVAAAEQALLAGADRVEGCLFGNGERTGNVDLVTLALNFYSQGIDPKLSFPAIRDVVTTVEHCNGLPV
HPRHPYAGELVHTAFSGSHQDAIRKGFTAHRQRNDGIWEMPYLPVDPADIGETYEAVIRVNSQSGKGGVAWVIEQDKGLK
LPRSLQRDLSRRVQEFSEKSGKEITAAMIWSIFQQAYHLTGPQEFELISYQDGNSRKAGNHRVFAGRIRHKGREIALSGR
GNGLISSVVAALEDGCGVALDVVDFHEHALKRGSDSQAVAYLECRTQDDRPIFGVGIDDDIATASVKAVLSAASNG
>Mature_556_residues
MLTNPEIKYRPFVSPIELDDRKWPSKRLTKAPRWLSTDLRDGNQALSDPMDVERKMRFYAMLLAIGFKEIEIAFPSASQT
EFDFVRALIGGNRIPDDVTIQVLTPSRQDLIARTFESLEGARQVIVHLYNATAPLFRRVVFGMERHEIVDLAITGVSAML
EESLRRPETDWTFEYSPETFCFTEPDFALEICERVLDVWQPTPERPAILNLPATVEVATPNVYADQIEWFCRNISRREAV
VISIHPHNDRGTAVAAAEQALLAGADRVEGCLFGNGERTGNVDLVTLALNFYSQGIDPKLSFPAIRDVVTTVEHCNGLPV
HPRHPYAGELVHTAFSGSHQDAIRKGFTAHRQRNDGIWEMPYLPVDPADIGETYEAVIRVNSQSGKGGVAWVIEQDKGLK
LPRSLQRDLSRRVQEFSEKSGKEITAAMIWSIFQQAYHLTGPQEFELISYQDGNSRKAGNHRVFAGRIRHKGREIALSGR
GNGLISSVVAALEDGCGVALDVVDFHEHALKRGSDSQAVAYLECRTQDDRPIFGVGIDDDIATASVKAVLSAASNG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=526, Percent_Identity=23.9543726235741, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324225, Length=567, Percent_Identity=50.2645502645503, Blast_Score=571, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6324682, Length=567, Percent_Identity=47.7954144620811, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61907; Mature: 61907

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNPEIKYRPFVSPIELDDRKWPSKRLTKAPRWLSTDLRDGNQALSDPMDVERKMRFYA
CCCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCHHHCCCHHHHHHHHHHH
MLLAIGFKEIEIAFPSASQTEFDFVRALIGGNRIPDDVTIQVLTPSRQDLIARTFESLEG
HHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
ARQVIVHLYNATAPLFRRVVFGMERHEIVDLAITGVSAMLEESLRRPETDWTFEYSPETF
HHHHHHHHHHCHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCEE
CFTEPDFALEICERVLDVWQPTPERPAILNLPATVEVATPNVYADQIEWFCRNISRREAV
EECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCEE
VISIHPHNDRGTAVAAAEQALLAGADRVEGCLFGNGERTGNVDLVTLALNFYSQGIDPKL
EEEEECCCCCCCCHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCC
SFPAIRDVVTTVEHCNGLPVHPRHPYAGELVHTAFSGSHQDAIRKGFTAHRQRNDGIWEM
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCHHHHHCCCCCCEEC
PYLPVDPADIGETYEAVIRVNSQSGKGGVAWVIEQDKGLKLPRSLQRDLSRRVQEFSEKS
CCCCCCHHHHCHHEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
GKEITAAMIWSIFQQAYHLTGPQEFELISYQDGNSRKAGNHRVFAGRIRHKGREIALSGR
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHCCCCEEEEECC
GNGLISSVVAALEDGCGVALDVVDFHEHALKRGSDSQAVAYLECRTQDDRPIFGVGIDDD
CCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCC
IATASVKAVLSAASNG
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTNPEIKYRPFVSPIELDDRKWPSKRLTKAPRWLSTDLRDGNQALSDPMDVERKMRFYA
CCCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCHHHCCCHHHHHHHHHHH
MLLAIGFKEIEIAFPSASQTEFDFVRALIGGNRIPDDVTIQVLTPSRQDLIARTFESLEG
HHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
ARQVIVHLYNATAPLFRRVVFGMERHEIVDLAITGVSAMLEESLRRPETDWTFEYSPETF
HHHHHHHHHHCHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCEE
CFTEPDFALEICERVLDVWQPTPERPAILNLPATVEVATPNVYADQIEWFCRNISRREAV
EECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCEE
VISIHPHNDRGTAVAAAEQALLAGADRVEGCLFGNGERTGNVDLVTLALNFYSQGIDPKL
EEEEECCCCCCCCHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCC
SFPAIRDVVTTVEHCNGLPVHPRHPYAGELVHTAFSGSHQDAIRKGFTAHRQRNDGIWEM
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCHHHHHCCCCCCEEC
PYLPVDPADIGETYEAVIRVNSQSGKGGVAWVIEQDKGLKLPRSLQRDLSRRVQEFSEKS
CCCCCCHHHHCHHEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
GKEITAAMIWSIFQQAYHLTGPQEFELISYQDGNSRKAGNHRVFAGRIRHKGREIALSGR
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHCCCCEEEEECC
GNGLISSVVAALEDGCGVALDVVDFHEHALKRGSDSQAVAYLECRTQDDRPIFGVGIDDD
CCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCC
IATASVKAVLSAASNG
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA