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The map label for this gene is trpS

Identifier: 161777477

GI number: 161777477

Start: 391248

End: 392390

Strand: Direct

Name: trpS

Synonym: SSO0452

Alternate gene names: 161777477

Gene position: 391248-392390 (Clockwise)

Preceding gene: 15897382

Following gene: 15897385

Centisome position: 13.08

GC content: 36.66

Gene sequence:

>1143_bases
ATGCCAGATGAATTTACTGTAACTCCTTGGGAAGTTAAGGGTAAAGTTGATTATGATAAACTAATTGTTCAATTTGGTAC
TCAGAAAATTACAGAAGAGCTGAAACAAAGAATTAAGAACTTAGCTGGGGATTTGCATGTCATGCTCAGGAGAAACGTAT
TTTTTTCTCATAGGGATTTAGATTTAGTTTTAAATGACTATGAGAAAAGTAAAGGATTCTTCCTATATACTGGAAGAGCG
CCTTCCTTAGGTATGCATATAGGACATCTGATACCATTCATATTTACCAAATGGCTACAAGAGAAATTTAATGCTAATTT
ATACATTGAGATAACTGACGACGAGAAGTACATGAGAAATCCAGAATTTACATTAGATCAAACTAGGAGTTGGGCTTACG
ATAATATTTTAGATATAATCGCTGTTGGCTTTAATCCCGATAAAACGTTCATCTTCCAAGATACAGAGTACATAAGGAAT
ATGTATCCTATAACAGTGAAAATAGCAAAGAAGCTGACGTTTTCAGAAGTAAGAGCTACTTTTGGATTAGACGCATCCTC
AAATATAGGTCTCATATTTTACCCAGCCCTACAGATAGCTCCTACCATGTTTGAAAAGAAGAGATGTCTAATACCAGCCG
GTATAGATCAAGATCCCTATTGGAGATTGCAAAGGGATATAGCGGAAAGCCTTGGGTATTATAAGGCTGCGCAGATACAT
AGTAAATTCCTTCCCCCACTCACGGGTCCAGAGGGCAAGATGAGTTCTTCAAACCCAGAAACGGCAATATATCTTGTAGA
TGATCCTAAAACCGTGGAAAGGAAAATCATGAAATACGCATTTTCAGGGGGACAACCCACAATAGAGTTACATAGGAAAT
ATGGCGGAAACCCGGAAATAGATGTTCCCTTTCAGTGGTTATATTACTTCTTTGAGGAGGATGATAATAGGATTAAGGAG
ATTGAGGAGGAGTATAGATCAGGCAAGATGTTAACCGGTGAGTTAAAACAGATATTAATAGACAAACTAAATAATTTCTT
AGAAGAACACAGAAGAAGGAGGGAAGAAGCAAAAGAACTTGTACATGTATTTAAATATGATGGTAAATTAGCTAAGCAGA
TGTGGGAGAAGATTCACGAATAG

Upstream 100 bases:

>100_bases
GTCGGTACCGGATTAGCCTCTAAATCTAATTCCTAACCCTTGTAACCGCATATTTCACTTAATTTTAACCTTTTATATTT
TCATGTATTTTCTTACTACA

Downstream 100 bases:

>100_bases
GTCTATATAAACCCATTTTCTTTAAGTGATACACCACGAGATTAGTTGAAATATTGAGCTCTTTTGCGATCCTATATATA
CTATTTCCTTTAATATACTC

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPDEFTVTPWEVKGKVDYDKLIVQFGTQKITEELKQRIKNLAGDLHVMLRRNVFFSHRDLDLVLNDYEKSKGFFLYTGRA
PSLGMHIGHLIPFIFTKWLQEKFNANLYIEITDDEKYMRNPEFTLDQTRSWAYDNILDIIAVGFNPDKTFIFQDTEYIRN
MYPITVKIAKKLTFSEVRATFGLDASSNIGLIFYPALQIAPTMFEKKRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIH
SKFLPPLTGPEGKMSSSNPETAIYLVDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPEIDVPFQWLYYFFEEDDNRIKE
IEEEYRSGKMLTGELKQILIDKLNNFLEEHRRRREEAKELVHVFKYDGKLAKQMWEKIHE

Sequences:

>Translated_380_residues
MPDEFTVTPWEVKGKVDYDKLIVQFGTQKITEELKQRIKNLAGDLHVMLRRNVFFSHRDLDLVLNDYEKSKGFFLYTGRA
PSLGMHIGHLIPFIFTKWLQEKFNANLYIEITDDEKYMRNPEFTLDQTRSWAYDNILDIIAVGFNPDKTFIFQDTEYIRN
MYPITVKIAKKLTFSEVRATFGLDASSNIGLIFYPALQIAPTMFEKKRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIH
SKFLPPLTGPEGKMSSSNPETAIYLVDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPEIDVPFQWLYYFFEEDDNRIKE
IEEEYRSGKMLTGELKQILIDKLNNFLEEHRRRREEAKELVHVFKYDGKLAKQMWEKIHE
>Mature_379_residues
PDEFTVTPWEVKGKVDYDKLIVQFGTQKITEELKQRIKNLAGDLHVMLRRNVFFSHRDLDLVLNDYEKSKGFFLYTGRAP
SLGMHIGHLIPFIFTKWLQEKFNANLYIEITDDEKYMRNPEFTLDQTRSWAYDNILDIIAVGFNPDKTFIFQDTEYIRNM
YPITVKIAKKLTFSEVRATFGLDASSNIGLIFYPALQIAPTMFEKKRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIHS
KFLPPLTGPEGKMSSSNPETAIYLVDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPEIDVPFQWLYYFFEEDDNRIKEI
EEEYRSGKMLTGELKQILIDKLNNFLEEHRRRREEAKELVHVFKYDGKLAKQMWEKIHE

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI47419916, Length=385, Percent_Identity=48.8311688311688, Blast_Score=357, Evalue=9e-99,
Organism=Homo sapiens, GI47419914, Length=385, Percent_Identity=48.8311688311688, Blast_Score=357, Evalue=9e-99,
Organism=Homo sapiens, GI47419920, Length=385, Percent_Identity=48.8311688311688, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI47419918, Length=385, Percent_Identity=48.8311688311688, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI239750058, Length=143, Percent_Identity=39.1608391608392, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI310120693, Length=143, Percent_Identity=39.1608391608392, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI239755558, Length=143, Percent_Identity=39.1608391608392, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789786, Length=311, Percent_Identity=24.4372990353698, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI72001474, Length=383, Percent_Identity=44.3864229765013, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6324475, Length=378, Percent_Identity=46.031746031746, Blast_Score=344, Evalue=1e-95,
Organism=Drosophila melanogaster, GI17864462, Length=372, Percent_Identity=45.4301075268817, Blast_Score=326, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24645295, Length=372, Percent_Identity=45.4301075268817, Blast_Score=326, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_SULSO (Q97ZX0)

Other databases:

- EMBL:   AE006641
- PIR:   C90190
- RefSeq:   NP_341988.2
- ProteinModelPortal:   Q97ZX0
- SMR:   Q97ZX0
- GeneID:   1455588
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0452
- NMPDR:   fig|273057.1.peg.411
- HOGENOM:   HBG435805
- OMA:   KAVFGFT
- PhylomeDB:   Q97ZX0
- ProtClustDB:   PRK12285
- BioCyc:   SSOL273057:SSO0452-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_A
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- InterPro:   IPR020653
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 44692; Mature: 44561

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDEFTVTPWEVKGKVDYDKLIVQFGTQKITEELKQRIKNLAGDLHVMLRRNVFFSHRDL
CCCCCCCCCEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
DLVLNDYEKSKGFFLYTGRAPSLGMHIGHLIPFIFTKWLQEKFNANLYIEITDDEKYMRN
HHHEECHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCC
PEFTLDQTRSWAYDNILDIIAVGFNPDKTFIFQDTEYIRNMYPITVKIAKKLTFSEVRAT
CCCCHHCCCCHHHHHHHHHEEECCCCCCEEEECCHHHHHCCCCEEEEHHHHHHHHHHHHH
FGLDASSNIGLIFYPALQIAPTMFEKKRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIH
HCCCCCCCCCEEEECHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHH
SKFLPPLTGPEGKMSSSNPETAIYLVDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPEI
HHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCC
DVPFQWLYYFFEEDDNRIKEIEEEYRSGKMLTGELKQILIDKLNNFLEEHRRRREEAKEL
CCCHHHHEEEHHCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHVFKYDGKLAKQMWEKIHE
HHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PDEFTVTPWEVKGKVDYDKLIVQFGTQKITEELKQRIKNLAGDLHVMLRRNVFFSHRDL
CCCCCCCCEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
DLVLNDYEKSKGFFLYTGRAPSLGMHIGHLIPFIFTKWLQEKFNANLYIEITDDEKYMRN
HHHEECHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCC
PEFTLDQTRSWAYDNILDIIAVGFNPDKTFIFQDTEYIRNMYPITVKIAKKLTFSEVRAT
CCCCHHCCCCHHHHHHHHHEEECCCCCCEEEECCHHHHHCCCCEEEEHHHHHHHHHHHHH
FGLDASSNIGLIFYPALQIAPTMFEKKRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIH
HCCCCCCCCCEEEECHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHH
SKFLPPLTGPEGKMSSSNPETAIYLVDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPEI
HHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCC
DVPFQWLYYFFEEDDNRIKEIEEEYRSGKMLTGELKQILIDKLNNFLEEHRRRREEAKEL
CCCHHHHEEEHHCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHVFKYDGKLAKQMWEKIHE
HHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726