Click here to switch to the map view.

The map label for this gene is mnmC

Identifier: 161760566

GI number: 161760566

Start: 3104442

End: 3106511

Strand: Direct

Name: mnmC

Synonym: YPTB2627

Alternate gene names: 161760566

Gene position: 3104442-3106511 (Clockwise)

Preceding gene: 51596944

Following gene: 51596953

Centisome position: 65.43

GC content: 53.09

Gene sequence:

>2070_bases
GTGAACCAACGCCCAATCCAAACCGCAACACTCAGCTGGAATGAACAGGGTACACCTGTATCTGAACAGTTTGGTGACAT
CTATTTTTCCAATGAAGATGGTCTGGAAGAGACTCACCACGTTTTTCTCAAAGGGAATGGGTTTCCTGCACGCTTCGCTT
CCCACCCGCAGCAAAGCTGTATTTTTGCTGAAACGGGTTTTGGCACTGGTTTAAACTTCCTGACATTATGGCGTGATTTT
GCCCTATTTAGGCAGCAATCGCCCAATGCTACGCTTCGGCGGTTGCACTACATTAGTTTTGAAAAATATCCACTTCACGT
CGCCGATCTAGCCTCAGCCCATGCCCGTTGGCCGGAGTTAGCCTCTTTCGCTGAACAACTGCGAGCGCAGTGGCCCCTAC
CGCTGGCCGGTTGTCATCGTATTTTGCTGGCTGACGGCGCTATTACGTTGGATTTATGGTTTGGTGATGTCAATACCCTA
TTACCCACATTGGACGATAGCCTGAATAATCAGGTAGATGCCTGGTTCCTTGATGGCTTTGCTCCCGCCAAGAATCCAGA
TATGTGGAATGAGCAGTTGTTCAATGCCATGGCGCGAATGACCAGGCCGGGAGGCACTTTTTCAACCTTTACCGCCGCCG
GATTTGTGCGTCGTGGTTTGCAGCAGGCAGGGTTTAACGTAACTAAAGTGAAAGGTTTCGGGCAAAAACGGGAAATGCTG
ACGGGCACCCTGCCACAACAGATTCATGCGCCCACGGCCCCTTGGTATCATCGGCCTGCCGCCACGCGCTGTGATGATAT
TGCCATTATCGGTGGCGGGATTGTCAGCGCCTTGACCGCACTGGCCTTACAACGTCGCGGGGCGGTGGTCACGCTCTACT
GTGCTGACGCACAGCCAGCACAGGGGGCATCAGGCAACCGACAAGGGGCACTCTACCCGTTGCTCAATGGTAAAAATGAT
GCACTGGAAACCTTTTTTACCAGTGCCTTTACCTTTGCCCGCCGTCAGTACGACCAACTGCTTGAGCAAGGGATCGCCTT
TGACCATCAGTGGTGCGGTGTCAGCCAATTAGCGTTTGATGATAAGAGCCGTGGCAAAATTGAGAAGATGCTGCATACAC
AGTGGCCAGTTGAGTTTGCCGAAGCGATGAGCCGTGAGCAACTCAGTGAGTTGGCCGGGCTGGATTGCGCCCATGATGGC
ATACACTATCCTGCTGGCGGTTGGCTCTGCCCTTCTGACCTCACTCACGCACTGATGATGCTAGCGCAACAACATGGCAT
GACCTGCCACTATCAACATGAACTGCAGCGATTAAAACGCATTGATAACCAGTGGCAACTGACCTTTGGTCAATCACAAG
CGGCTAAACATCATGCCACCGTCATTTTAGCAACTGGCCACCGCTTACCTGAATGGGAACAAACTCACCATCTGCCCTTG
TCTGCGGTACGTGGGCAGGTGTCACACATCCCGACCACCCCGGTGCTCAGCCAACTGCAACAGGTGTTGTGCTATGACGG
TTACCTGACGCCAGTCAACCCAGCCAATCAGCATCATTGTATTGGTGCCAGTTATCAGCGCGGCGACATCGCGACTGATT
TTCGCCTAACCGAGCAGCAGGAGAACCGTGAGCGTTTGCTACGCTGTCTGCCACAAGTCAGTTGGCCGCAGCAGGTAGAT
GTCAGCGATAATCAGGCTCGGTGTGGCGTTCGCTGCGCTATCCGCGACCATTTACCGATGGTCGGTGCCGTGCCGGATTA
TGCTGCAACACTGGCTCAATATCAGGATCTGTCTCGTCGTATTCAGCATGGGGGTGAGAGCGAGGTAAATGATATCGCTG
TCGCGCCAGTCTGGCCTGAGTTATTTATGGTCGGGGGGTTAGGCTCGCGTGGCTTATGCAGTGCACCATTGGTGGCCGAG
ATTCTGGCCGCGCAGATGTTCGGTGAACCATTGCCGTTAGATGCCAAAACGCTGGCAGCATTGAATCCTAACCGTTTTTG
GATCAAAAAATTATTGAAAGGCAGGCCGGTACAAACTCGCTCCCCGGCCACCCAAGAGTCATCAAGATGA

Upstream 100 bases:

>100_bases
AAGTCCGATCAGCGTAATATAGCCAGAAAGTTTTACAATTTGCACGTGGAACACCACCTCGTTAGAATCGCTCTACCCCT
TGAGATACGAGTCTTCTAAC

Downstream 100 bases:

>100_bases
GTGACAAGTGCCGCTAACTATCACGCCCCCACGGCTTCAAGAACGAAGGGGAACGCCCAAAGTTATTGGCGTCGCAGGTA
GGCAGCAAGTAAATGAATCC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MNQRPIQTATLSWNEQGTPVSEQFGDIYFSNEDGLEETHHVFLKGNGFPARFASHPQQSCIFAETGFGTGLNFLTLWRDF
ALFRQQSPNATLRRLHYISFEKYPLHVADLASAHARWPELASFAEQLRAQWPLPLAGCHRILLADGAITLDLWFGDVNTL
LPTLDDSLNNQVDAWFLDGFAPAKNPDMWNEQLFNAMARMTRPGGTFSTFTAAGFVRRGLQQAGFNVTKVKGFGQKREML
TGTLPQQIHAPTAPWYHRPAATRCDDIAIIGGGIVSALTALALQRRGAVVTLYCADAQPAQGASGNRQGALYPLLNGKND
ALETFFTSAFTFARRQYDQLLEQGIAFDHQWCGVSQLAFDDKSRGKIEKMLHTQWPVEFAEAMSREQLSELAGLDCAHDG
IHYPAGGWLCPSDLTHALMMLAQQHGMTCHYQHELQRLKRIDNQWQLTFGQSQAAKHHATVILATGHRLPEWEQTHHLPL
SAVRGQVSHIPTTPVLSQLQQVLCYDGYLTPVNPANQHHCIGASYQRGDIATDFRLTEQQENRERLLRCLPQVSWPQQVD
VSDNQARCGVRCAIRDHLPMVGAVPDYAATLAQYQDLSRRIQHGGESEVNDIAVAPVWPELFMVGGLGSRGLCSAPLVAE
ILAAQMFGEPLPLDAKTLAALNPNRFWIKKLLKGRPVQTRSPATQESSR

Sequences:

>Translated_689_residues
MNQRPIQTATLSWNEQGTPVSEQFGDIYFSNEDGLEETHHVFLKGNGFPARFASHPQQSCIFAETGFGTGLNFLTLWRDF
ALFRQQSPNATLRRLHYISFEKYPLHVADLASAHARWPELASFAEQLRAQWPLPLAGCHRILLADGAITLDLWFGDVNTL
LPTLDDSLNNQVDAWFLDGFAPAKNPDMWNEQLFNAMARMTRPGGTFSTFTAAGFVRRGLQQAGFNVTKVKGFGQKREML
TGTLPQQIHAPTAPWYHRPAATRCDDIAIIGGGIVSALTALALQRRGAVVTLYCADAQPAQGASGNRQGALYPLLNGKND
ALETFFTSAFTFARRQYDQLLEQGIAFDHQWCGVSQLAFDDKSRGKIEKMLHTQWPVEFAEAMSREQLSELAGLDCAHDG
IHYPAGGWLCPSDLTHALMMLAQQHGMTCHYQHELQRLKRIDNQWQLTFGQSQAAKHHATVILATGHRLPEWEQTHHLPL
SAVRGQVSHIPTTPVLSQLQQVLCYDGYLTPVNPANQHHCIGASYQRGDIATDFRLTEQQENRERLLRCLPQVSWPQQVD
VSDNQARCGVRCAIRDHLPMVGAVPDYAATLAQYQDLSRRIQHGGESEVNDIAVAPVWPELFMVGGLGSRGLCSAPLVAE
ILAAQMFGEPLPLDAKTLAALNPNRFWIKKLLKGRPVQTRSPATQESSR
>Mature_689_residues
MNQRPIQTATLSWNEQGTPVSEQFGDIYFSNEDGLEETHHVFLKGNGFPARFASHPQQSCIFAETGFGTGLNFLTLWRDF
ALFRQQSPNATLRRLHYISFEKYPLHVADLASAHARWPELASFAEQLRAQWPLPLAGCHRILLADGAITLDLWFGDVNTL
LPTLDDSLNNQVDAWFLDGFAPAKNPDMWNEQLFNAMARMTRPGGTFSTFTAAGFVRRGLQQAGFNVTKVKGFGQKREML
TGTLPQQIHAPTAPWYHRPAATRCDDIAIIGGGIVSALTALALQRRGAVVTLYCADAQPAQGASGNRQGALYPLLNGKND
ALETFFTSAFTFARRQYDQLLEQGIAFDHQWCGVSQLAFDDKSRGKIEKMLHTQWPVEFAEAMSREQLSELAGLDCAHDG
IHYPAGGWLCPSDLTHALMMLAQQHGMTCHYQHELQRLKRIDNQWQLTFGQSQAAKHHATVILATGHRLPEWEQTHHLPL
SAVRGQVSHIPTTPVLSQLQQVLCYDGYLTPVNPANQHHCIGASYQRGDIATDFRLTEQQENRERLLRCLPQVSWPQQVD
VSDNQARCGVRCAIRDHLPMVGAVPDYAATLAQYQDLSRRIQHGGESEVNDIAVAPVWPELFMVGGLGSRGLCSAPLVAE
ILAAQMFGEPLPLDAKTLAALNPNRFWIKKLLKGRPVQTRSPATQESSR

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=680, Percent_Identity=60.4411764705882, Blast_Score=824, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 76694; Mature: 76694

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRPIQTATLSWNEQGTPVSEQFGDIYFSNEDGLEETHHVFLKGNGFPARFASHPQQSC
CCCCCCEEEEEECCCCCCCHHHHHCCEEECCCCCCCCCCEEEEECCCCCCHHHCCCCCCE
IFAETGFGTGLNFLTLWRDFALFRQQSPNATLRRLHYISFEKYPLHVADLASAHARWPEL
EEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHH
ASFAEQLRAQWPLPLAGCHRILLADGAITLDLWFGDVNTLLPTLDDSLNNQVDAWFLDGF
HHHHHHHHHCCCCCHHHHCEEEEECCEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCC
APAKNPDMWNEQLFNAMARMTRPGGTFSTFTAAGFVRRGLQQAGFNVTKVKGFGQKREML
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TGTLPQQIHAPTAPWYHRPAATRCDDIAIIGGGIVSALTALALQRRGAVVTLYCADAQPA
HCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
QGASGNRQGALYPLLNGKNDALETFFTSAFTFARRQYDQLLEQGIAFDHQWCGVSQLAFD
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCC
DKSRGKIEKMLHTQWPVEFAEAMSREQLSELAGLDCAHDGIHYPAGGWLCPSDLTHALMM
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHH
LAQQHGMTCHYQHELQRLKRIDNQWQLTFGQSQAAKHHATVILATGHRLPEWEQTHHLPL
HHHHCCCEEEHHHHHHHHHHCCCCEEEEECCHHHCCCCEEEEEEECCCCCCCHHHCCCCH
SAVRGQVSHIPTTPVLSQLQQVLCYDGYLTPVNPANQHHCIGASYQRGDIATDFRLTEQQ
HHHCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCHH
ENRERLLRCLPQVSWPQQVDVSDNQARCGVRCAIRDHLPMVGAVPDYAATLAQYQDLSRR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHH
IQHGGESEVNDIAVAPVWPELFMVGGLGSRGLCSAPLVAEILAAQMFGEPLPLDAKTLAA
HHCCCCCCCCCEEECCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
LNPNRFWIKKLLKGRPVQTRSPATQESSR
CCCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQRPIQTATLSWNEQGTPVSEQFGDIYFSNEDGLEETHHVFLKGNGFPARFASHPQQSC
CCCCCCEEEEEECCCCCCCHHHHHCCEEECCCCCCCCCCEEEEECCCCCCHHHCCCCCCE
IFAETGFGTGLNFLTLWRDFALFRQQSPNATLRRLHYISFEKYPLHVADLASAHARWPEL
EEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHH
ASFAEQLRAQWPLPLAGCHRILLADGAITLDLWFGDVNTLLPTLDDSLNNQVDAWFLDGF
HHHHHHHHHCCCCCHHHHCEEEEECCEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCC
APAKNPDMWNEQLFNAMARMTRPGGTFSTFTAAGFVRRGLQQAGFNVTKVKGFGQKREML
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TGTLPQQIHAPTAPWYHRPAATRCDDIAIIGGGIVSALTALALQRRGAVVTLYCADAQPA
HCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
QGASGNRQGALYPLLNGKNDALETFFTSAFTFARRQYDQLLEQGIAFDHQWCGVSQLAFD
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCC
DKSRGKIEKMLHTQWPVEFAEAMSREQLSELAGLDCAHDGIHYPAGGWLCPSDLTHALMM
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHH
LAQQHGMTCHYQHELQRLKRIDNQWQLTFGQSQAAKHHATVILATGHRLPEWEQTHHLPL
HHHHCCCEEEHHHHHHHHHHCCCCEEEEECCHHHCCCCEEEEEEECCCCCCCHHHCCCCH
SAVRGQVSHIPTTPVLSQLQQVLCYDGYLTPVNPANQHHCIGASYQRGDIATDFRLTEQQ
HHHCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCHH
ENRERLLRCLPQVSWPQQVDVSDNQARCGVRCAIRDHLPMVGAVPDYAATLAQYQDLSRR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHH
IQHGGESEVNDIAVAPVWPELFMVGGLGSRGLCSAPLVAEILAAQMFGEPLPLDAKTLAA
HHCCCCCCCCCEEECCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
LNPNRFWIKKLLKGRPVQTRSPATQESSR
CCCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA