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The map label for this gene is trpS

Identifier: 161751105

GI number: 161751105

Start: 195440

End: 196585

Strand: Direct

Name: trpS

Synonym: ST0169

Alternate gene names: 161751105

Gene position: 195440-196585 (Clockwise)

Preceding gene: 15920347

Following gene: 15920350

Centisome position: 7.25

GC content: 32.11

Gene sequence:

>1146_bases
ATGGCTCAAGATTTTAATGTTACCCCTTGGGAAGTAAAAGGAAAAGTAGATTATGATAAGCTAATTGTCCAATTTGGAAC
TCAAAAAATTACTTCAGAATTAAAGGAAAAGATTAAGAGTATTATTAATGATGAACTCCACGTTATGTTAAGAAGAGATG
TTTTCTTTTCTCATAGGGATTTAGATTTAGTTTTAAAAGATTATCAAGATGGTAAAGGATTTTTCCTATATACTGGAAGA
GCCCCTTCATTAGGTATGCATATAGGTCATTTAATTCCTTTTATCTTTACTAAATGGTTACAAGACAAATTTAACGTTAA
TCTATATATAGAGATAACAGACGATGAAAAATTTATGAGGAATCCAGAATATACTTTGGATCAAACTAGACAATGGGCGT
ATGATAATATTTTAGATATAATAGCTGTTGGATTTAACCCAGATAAAACTTTCATATTTCAAGATACAGAGTATATTAGA
AATATGTACCCTATTGCAATAAAAATTGCTAAAAAATTAACATTCTCCGAAGTTAGGGCTACGTTTGGTTTAGATACTTC
TTCAAATATAGGAATAATTTGGTATCCAGCATTACAAATAGCCCCTACAATGTTTGAGAAGAGGAGATGTCTAATACCAG
CTGGTATTGACCAAGATCCTTATTGGAGATTGCAAAGAGATATTGCTGAGAGCCTAGGTTATTATAAGGCAGCACAGATT
CATAGTAAATTCCTTCCTCCATTAACTGGACCCGAAGGAAAAATGAGCTCATCTCAGCCTGAGACAGCAATTTATCTTAC
TGATGATCCTAAGACTGTTGAAAGGAAAATAATGAAATATGCATTTTCTGGAGGTCAACCTACAATTGAATTGCATAGAA
AATATGGTGGTAATCCAGACATAGATGTGTCATTTCAGTGGCTGTATATGTTCTTTGAGCCTGATGATAATAAGATAAAG
AAAATAGAAGAAGATTATAGATCTGGTGCACTTTTAACTGGTGAACTAAAACAGATATTAATAGAAAAATTAAATGATTT
CTTGGAAGAACATAGGCAGAAAAGAGAAGAAGCTAAGAAATTGGTGAATGTTTTCAAATATGATGGAGAACTTGCTAGAG
AGATGTGGAGGAAGATTCACGAATAA

Upstream 100 bases:

>100_bases
GAACAATTCCCAGAGCTACCAGCTGGTGGATTGGCATCTTCACAAAAATCATGACCTTTCTTACCACATTTTTAAATTTC
TCATTTTTCTTTTTTATATT

Downstream 100 bases:

>100_bases
TACCGTATTTCTTTAAGTAGTATACGATTAGGTTAGTTGATCTATTCAACTTTTTAGCAATCTCGTATATTGATTTACCC
TCTTTATACATACTAATAAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAQDFNVTPWEVKGKVDYDKLIVQFGTQKITSELKEKIKSIINDELHVMLRRDVFFSHRDLDLVLKDYQDGKGFFLYTGR
APSLGMHIGHLIPFIFTKWLQDKFNVNLYIEITDDEKFMRNPEYTLDQTRQWAYDNILDIIAVGFNPDKTFIFQDTEYIR
NMYPIAIKIAKKLTFSEVRATFGLDTSSNIGIIWYPALQIAPTMFEKRRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQI
HSKFLPPLTGPEGKMSSSQPETAIYLTDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPDIDVSFQWLYMFFEPDDNKIK
KIEEDYRSGALLTGELKQILIEKLNDFLEEHRQKREEAKKLVNVFKYDGELAREMWRKIHE

Sequences:

>Translated_381_residues
MAQDFNVTPWEVKGKVDYDKLIVQFGTQKITSELKEKIKSIINDELHVMLRRDVFFSHRDLDLVLKDYQDGKGFFLYTGR
APSLGMHIGHLIPFIFTKWLQDKFNVNLYIEITDDEKFMRNPEYTLDQTRQWAYDNILDIIAVGFNPDKTFIFQDTEYIR
NMYPIAIKIAKKLTFSEVRATFGLDTSSNIGIIWYPALQIAPTMFEKRRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQI
HSKFLPPLTGPEGKMSSSQPETAIYLTDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPDIDVSFQWLYMFFEPDDNKIK
KIEEDYRSGALLTGELKQILIEKLNDFLEEHRQKREEAKKLVNVFKYDGELAREMWRKIHE
>Mature_380_residues
AQDFNVTPWEVKGKVDYDKLIVQFGTQKITSELKEKIKSIINDELHVMLRRDVFFSHRDLDLVLKDYQDGKGFFLYTGRA
PSLGMHIGHLIPFIFTKWLQDKFNVNLYIEITDDEKFMRNPEYTLDQTRQWAYDNILDIIAVGFNPDKTFIFQDTEYIRN
MYPIAIKIAKKLTFSEVRATFGLDTSSNIGIIWYPALQIAPTMFEKRRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIH
SKFLPPLTGPEGKMSSSQPETAIYLTDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPDIDVSFQWLYMFFEPDDNKIKK
IEEDYRSGALLTGELKQILIEKLNDFLEEHRQKREEAKKLVNVFKYDGELAREMWRKIHE

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI47419916, Length=373, Percent_Identity=50.1340482573727, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI47419914, Length=373, Percent_Identity=50.1340482573727, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI47419920, Length=373, Percent_Identity=50.1340482573727, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI47419918, Length=373, Percent_Identity=50.1340482573727, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI239750058, Length=143, Percent_Identity=42.6573426573427, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI310120693, Length=143, Percent_Identity=42.6573426573427, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239755558, Length=143, Percent_Identity=41.958041958042, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI72001474, Length=366, Percent_Identity=44.5355191256831, Blast_Score=317, Evalue=6e-87,
Organism=Saccharomyces cerevisiae, GI6324475, Length=373, Percent_Identity=46.112600536193, Blast_Score=342, Evalue=5e-95,
Organism=Drosophila melanogaster, GI17864462, Length=367, Percent_Identity=45.7765667574932, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24645295, Length=367, Percent_Identity=45.7765667574932, Blast_Score=322, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_SULTO (Q976M1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376017.2
- ProteinModelPortal:   Q976M1
- SMR:   Q976M1
- GeneID:   1458052
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0169
- NMPDR:   fig|273063.1.peg.185
- HOGENOM:   HBG435805
- ProtClustDB:   PRK12285
- BioCyc:   STOK273063:ST0169-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_A
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- InterPro:   IPR020653
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 44719; Mature: 44588

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDFNVTPWEVKGKVDYDKLIVQFGTQKITSELKEKIKSIINDELHVMLRRDVFFSHRD
CCCCCCCCCEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
LDLVLKDYQDGKGFFLYTGRAPSLGMHIGHLIPFIFTKWLQDKFNVNLYIEITDDEKFMR
HHHHEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHC
NPEYTLDQTRQWAYDNILDIIAVGFNPDKTFIFQDTEYIRNMYPIAIKIAKKLTFSEVRA
CCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHHHHHCHHHHHHHHHHHHHHHHH
TFGLDTSSNIGIIWYPALQIAPTMFEKRRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQI
HHCCCCCCCCCEEEECHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHHHH
HSKFLPPLTGPEGKMSSSQPETAIYLTDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPD
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCCC
IDVSFQWLYMFFEPDDNKIKKIEEDYRSGALLTGELKQILIEKLNDFLEEHRQKREEAKK
CEEEEEEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVNVFKYDGELAREMWRKIHE
HHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AQDFNVTPWEVKGKVDYDKLIVQFGTQKITSELKEKIKSIINDELHVMLRRDVFFSHRD
CCCCCCCCEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
LDLVLKDYQDGKGFFLYTGRAPSLGMHIGHLIPFIFTKWLQDKFNVNLYIEITDDEKFMR
HHHHEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHC
NPEYTLDQTRQWAYDNILDIIAVGFNPDKTFIFQDTEYIRNMYPIAIKIAKKLTFSEVRA
CCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHHHHHCHHHHHHHHHHHHHHHHH
TFGLDTSSNIGIIWYPALQIAPTMFEKRRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQI
HHCCCCCCCCCEEEECHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHHHH
HSKFLPPLTGPEGKMSSSQPETAIYLTDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPD
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCCC
IDVSFQWLYMFFEPDDNKIKKIEEDYRSGALLTGELKQILIEKLNDFLEEHRQKREEAKK
CEEEEEEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVNVFKYDGELAREMWRKIHE
HHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479