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The map label for this gene is proB

Identifier: 161723303

GI number: 161723303

Start: 2136770

End: 2137873

Strand: Direct

Name: proB

Synonym: PM1896

Alternate gene names: 161723303

Gene position: 2136770-2137873 (Clockwise)

Preceding gene: 15603757

Following gene: 15603762

Centisome position: 94.65

GC content: 45.83

Gene sequence:

>1104_bases
ATGAAAATCGACAAAACACTCGTTGTGAAATTTGGAACCAGTACGCTTACGCAAGGATCGGCGAAGTTAAATTTACCTCA
TATGATGGATATTGTGCGTCAGCTCGCACAGCTTCATCAAGCGGGCTTTCGTTTAGTGATTGTCACCTCAGGGGCGATTG
CGGCAGGTCGTCATTATTTAAATCACCCGCAATTGCCGCCGACGATTGCCTCGAAACAATTATTGGCTGCGGTAGGACAA
AGTCAGCTCATTCAAACGTGGGAAAAATTGTTTGCCATTTATGATATTCATATTGGGCAGATTTTATTGACCCGTGCCGA
CATTGAAGATCGTGAACGTTTTTTAAATGCCAGAGATACTTTGCGCGCCTTATTAGATAATCAGATTATTCCAGTGATTA
ATGAAAATGACGCTGTCGCTACGTCAGAAATTAAAGTAGGCGATAACGATAATTTATCCGCGTTAGTGGCGATTTTAGTG
CAAGCAGAACAGTTGTATTTGCTTACTGATCAGCAAGGTTTGTTTGATCGTGATCCGCGCAAACATCCAGACGCCAAATT
GATCGCCGACGTGGACAAAATTACCGATCATATTCGCGCGATTGCGGGCGGAAGTGGCACCTCGTTAGGCACAGGCGGCA
TGTCCACCAAAATCAGTGCCGCGGATGTGGCAACCCGCTCCGGCATTGAAACTATTATTGCACCGGGTAATCGCCCGAAT
GTGATTGTGGACTTAGCTTATGGGCAAGCCATTGGCACCAAATTTAGTGTGCAAGCAGATCGCTTAGAAAGTCGTAAACA
ATGGCTATATGCGGCGCCGTCAGCCGGTATTATTACCATTGATGCGGGGGCGGAAAACGCGATGTTAATGCAACATAAAT
CGTTATTGCCTGCTGGGATTGTGAACGTGGAAGGACGCTTTTCACGCGGTGAAGTGGTAAAAATTCGTACTCAGCAAGGT
AAGGATGTCGCCTTAGGGATGCCACGTTATAACAGCGATGCACTCCATTTAATTCAAGGCAAACATTCGCAAGATATCGA
ACAGGTTCTCGGTTATGAATATGGGGCGGTTGCGGTACACCGCGACGATATGATTGTGTTATAA

Upstream 100 bases:

>100_bases
GCTAAATCATAGAAAATAGCAAGTAAACGAGATGATTTTTCTGATGATTAATGGTAAGTTAGCTTCAATTTTGTGCGACA
AAAATAAGAAGATAGAGAAG

Downstream 100 bases:

>100_bases
ATCAGAAAAAGAAAGGTAACAAGATGAATTGGAAAGCAGTATTGATATTCAGTGTACTAGGTTCGCTGGCGAGCTGTGCT
TCATACTCTGAACATGCGGT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKIDKTLVVKFGTSTLTQGSAKLNLPHMMDIVRQLAQLHQAGFRLVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQ
SQLIQTWEKLFAIYDIHIGQILLTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKISAADVATRSGIETIIAPGNRPN
VIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAGIITIDAGAENAMLMQHKSLLPAGIVNVEGRFSRGEVVKIRTQQG
KDVALGMPRYNSDALHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL

Sequences:

>Translated_367_residues
MKIDKTLVVKFGTSTLTQGSAKLNLPHMMDIVRQLAQLHQAGFRLVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQ
SQLIQTWEKLFAIYDIHIGQILLTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKISAADVATRSGIETIIAPGNRPN
VIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAGIITIDAGAENAMLMQHKSLLPAGIVNVEGRFSRGEVVKIRTQQG
KDVALGMPRYNSDALHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL
>Mature_367_residues
MKIDKTLVVKFGTSTLTQGSAKLNLPHMMDIVRQLAQLHQAGFRLVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQ
SQLIQTWEKLFAIYDIHIGQILLTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKISAADVATRSGIETIIAPGNRPN
VIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAGIITIDAGAENAMLMQHKSLLPAGIVNVEGRFSRGEVVKIRTQQG
KDVALGMPRYNSDALHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=289, Percent_Identity=33.5640138408304, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI21361368, Length=291, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=66.5753424657534, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=32.7205882352941, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=32.7205882352941, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=34.8668280871671, Blast_Score=222, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6321822, Length=412, Percent_Identity=34.9514563106796, Blast_Score=212, Evalue=9e-56,
Organism=Drosophila melanogaster, GI21357643, Length=333, Percent_Identity=31.2312312312312, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_PASMU (Q9CJU5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246835.2
- ProteinModelPortal:   Q9CJU5
- SMR:   Q9CJU5
- GeneID:   1245243
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1896
- NMPDR:   fig|272843.1.peg.1897
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   PMUL272843:PM1896-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39895; Mature: 39895

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDKTLVVKFGTSTLTQGSAKLNLPHMMDIVRQLAQLHQAGFRLVIVTSGAIAAGRHYL
CCCCCEEEEEECCCHHCCCCCEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
NHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQILLTRADIEDRERFLNARDT
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHEECCEEEEEECCHHHHHHHHHHHHH
LRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILVQAEQLYLLTDQQGLFDRDPR
HHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCC
KHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKISAADVATRSGIETIIAPGNRPN
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHCCCEEEECCCCCCC
VIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAGIITIDAGAENAMLMQHKSLLPAGI
EEEEEECCCCCCCEEEEEHHHHHHHHHEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCE
VNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDALHLIQGKHSQDIEQVLGYEYGAVAVH
EECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEE
RDDMIVL
CCCEEEC
>Mature Secondary Structure
MKIDKTLVVKFGTSTLTQGSAKLNLPHMMDIVRQLAQLHQAGFRLVIVTSGAIAAGRHYL
CCCCCEEEEEECCCHHCCCCCEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
NHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQILLTRADIEDRERFLNARDT
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHEECCEEEEEECCHHHHHHHHHHHHH
LRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILVQAEQLYLLTDQQGLFDRDPR
HHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCC
KHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKISAADVATRSGIETIIAPGNRPN
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHCCCEEEECCCCCCC
VIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAGIITIDAGAENAMLMQHKSLLPAGI
EEEEEECCCCCCCEEEEEHHHHHHHHHEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCE
VNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDALHLIQGKHSQDIEQVLGYEYGAVAVH
EECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEE
RDDMIVL
CCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100