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The map label for this gene is metG

Identifier: 161723239

GI number: 161723239

Start: 3261809

End: 3263821

Strand: Reverse

Name: metG

Synonym: lpg2882

Alternate gene names: 161723239

Gene position: 3263821-3261809 (Counterclockwise)

Preceding gene: 52843079

Following gene: 52843076

Centisome position: 96.06

GC content: 41.78

Gene sequence:

>2013_bases
ATGACTAGTGAACGAAAAATGTTAGTGACCAGTGCACTGCCTTATGCTAATGGGCATTTGCATCTGGGACATTTGGTTGA
GCATATTCAAACAGATATTTGGGTCCGTACTCACAAAATGTTGGGTATTCAATGTATTAGCGTTTGTGGGGATGACGCTC
ATGGAACACCCATTATGCTGAAAGCAGAGCAACTGGGAATTACTCCGGAAGCTTTAACCGCCGAAATTAAGTTAAGTCAT
GAAAAGGATTTTAAAGCCTTCGCCATTGATTATGATTATTACCATACCACCCATTCACCTGAAAATCAGGCATTGGCGAC
TACCATATTTGAACGTTTGCAGGCAGGCGGTGATATAGTCAAGAAAACAATTCGCCAGTTTTATGATCCCGTAAAGCAAA
TGTTTTTGCCGGACAGGTATGTGAAGGGAACTTGCCCCAAATGTGCTGCTGTCGATCAGTATGGCGATAATTGCGAGGTT
TGTGGCGCCACTTATTCGCCGACGGATTTGATTAACCCTGTTTCTGTTATATCGGGAGTCAGCCCAGTAGAAAAAGAATC
AGAGCATTATTTTTTTGATTTGCCGCGTTACGAAGAGTTACTTAAGGATTGGACAAGAAAGGGGCATTTACAAACCGAAG
TAACGAATAAACTCAGCGAATGGTTTGAGGCGGGTTTAAAGCAATGGGATATTTCTCGCGATGCTCCCTATTTTGGCTTC
CCTATCCCCGGTGTTCCGGATAAATACTTTTACGTTTGGTTAGATGCTCCCATAGGCTATATGGCAAGCTTTAAAAAGTA
TTGCGATGAGCGTGGCGTTTCTTTTGATGAGTTTTGGGATAAGGCTTCAAAAACAGAATTATATCATTTTGTAGGCAAGG
ATATTGTGTATTTTCATGCTTTGTTCTGGCCAGCTATGTTAGCTGCCAGCGGTTTCAGAACACCTACGGCCGTGTATACC
CATGGGTTTTTAACGGTTGAGGGGCAGAAAATGTCCAAATCGCGCGGTACTTTTATAGAAGCACGCGCTTATCTGGCTCA
CTTGCATCCTGAATATCTGCGTTACTATTTCGCTGCCAAATTGAATGGTCGAGTAGACGATTTGGATTTGAATTTTGACG
ATTTTGTCAATAGGGTGAATGCGGATCTGGTCGGGAAGGTAGTGAATATTGCAAGCCGATGTGCGGGATTTATTAATAAG
CGTTTTGATAATCGGTTGAGCTCAGAGTTGAATGATCAAAAATTATACAACGACTTGCTGAGCGCAAGAGAGTCCGTGAT
TGACGCATTTGTTTCAAGAGACTATGCGCGGGCAATAAGACAAATCATGGATTGTGCTGACAAAGTCAATCAATACATCG
ATGCCAATAAACCTTGGGTATTGGCTAAAGATGAAAGCAAATTAAATGAAGTTCATGCCATATGCACAATGGGAATCAAT
TTATTCCGCATTTTAATAACCTATTTAAAACCAGTCTTGCCAATGATGGCCAAAGCTTCAGAAGAATTTTTAAATTCTGA
GCCTTTACACTGGGATTCTATCGATAAACCTTTGCTAAACCATCGTATCAATACTTTTAAGCCTTTGATGGTTAGAGTAG
AAAAAGAAAAAATTGAAGCGATGTTGGTTCAAAGCAAGGAATCTCTGATGACTACCCCAGTAAAAGAAAACACGCCAGTT
GAGGACGCTAACTTAATCAGTATTGAGGACTTTGCCAAAGTCGATTTACGGATTGCAAAAATTGTCAATGCAGAGCCTGT
GGAAGGCGCAGATAAACTAATGCGCTTGATTTTAGACTTGGGTGATGCTCAAAAGCAGGTTTTTGCCGGCATAAAAAAGG
CTTATGATGCTGAAGAATTGATTGGGCGTTTGACAGTAATGGTTGCTAATTTGGAGCCCCGCACTATGCGTTTCGGTGTT
TCTGAAGGCATGGTATTGGCGGCAGGGGATGGGCAGGGAATTTATTTATTACAGCCTGATGCAGGAGCACTTCCTGGCAT
GAAGGTGAAGTAA

Upstream 100 bases:

>100_bases
TAAATAACTACCAATAAAAACCTGTTTTCTATTGCAGCATTTCCATTGTCTTTGCGTTATAATTAGTCGCTTTATTATAG
GTGATGAGTATTCTATAACT

Downstream 100 bases:

>100_bases
TGGCCTCTGTTAAAGAAATTGATGCGTTATTGCCGCAAACCCAGTGTGGGGAATGCGGATATGCAGGTTGTTTGCCTTAT
GCTGAAGCGCTTGCCCAAGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSH
EKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
CGATYSPTDLINPVSVISGVSPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYT
HGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINK
RFDNRLSSELNDQKLYNDLLSARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
LFRILITYLKPVLPMMAKASEEFLNSEPLHWDSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPVKENTPV
EDANLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGV
SEGMVLAAGDGQGIYLLQPDAGALPGMKVK

Sequences:

>Translated_670_residues
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSH
EKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
CGATYSPTDLINPVSVISGVSPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYT
HGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINK
RFDNRLSSELNDQKLYNDLLSARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
LFRILITYLKPVLPMMAKASEEFLNSEPLHWDSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPVKENTPV
EDANLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGV
SEGMVLAAGDGQGIYLLQPDAGALPGMKVK
>Mature_669_residues
TSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHE
KDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVC
GATYSPTDLINPVSVISGVSPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGFP
IPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTH
GFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKR
FDNRLSSELNDQKLYNDLLSARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGINL
FRILITYLKPVLPMMAKASEEFLNSEPLHWDSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPVKENTPVE
DANLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVS
EGMVLAAGDGQGIYLLQPDAGALPGMKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=562, Percent_Identity=27.4021352313167, Blast_Score=240, Evalue=4e-63,
Organism=Homo sapiens, GI24308436, Length=523, Percent_Identity=23.9005736137667, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=60.0297176820208, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=106, Percent_Identity=34.9056603773585, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=672, Percent_Identity=27.0833333333333, Blast_Score=219, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI212645096, Length=511, Percent_Identity=23.8747553816047, Blast_Score=149, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71992781, Length=405, Percent_Identity=24.1975308641975, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=29.8747763864043, Blast_Score=253, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=22.803738317757, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24655311, Length=565, Percent_Identity=30.0884955752212, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI21358483, Length=517, Percent_Identity=24.1779497098646, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75889; Mature: 75758

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIML
CCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIV
EHHHHCCCHHHHEEEEEECCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHCCHHHH
KKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHCCC
SPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
CCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHA
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
LFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAK
HHHHHHHHHCCCCCCCEEEECCEEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKRFDNRLSSELNDQKLYNDLL
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
LFRILITYLKPVLPMMAKASEEFLNSEPLHWDSIDKPLLNHRINTFKPLMVRVEKEKIEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHEEHHHHHHH
MLVQSKESLMTTPVKENTPVEDANLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDL
HHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPD
CHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCEEEEECCC
AGALPGMKVK
CCCCCCCCCC
>Mature Secondary Structure 
TSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIML
CCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIV
EHHHHCCCHHHHEEEEEECCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHCCHHHH
KKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHCCC
SPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
CCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHA
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
LFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAK
HHHHHHHHHCCCCCCCEEEECCEEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKRFDNRLSSELNDQKLYNDLL
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
LFRILITYLKPVLPMMAKASEEFLNSEPLHWDSIDKPLLNHRINTFKPLMVRVEKEKIEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHEEHHHHHHH
MLVQSKESLMTTPVKENTPVEDANLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDL
HHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPD
CHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCEEEEECCC
AGALPGMKVK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA