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The map label for this gene is hisF [H]

Identifier: 161723193

GI number: 161723193

Start: 3734883

End: 3735656

Strand: Reverse

Name: hisF [H]

Synonym: BPSL3133

Alternate gene names: 161723193

Gene position: 3735656-3734883 (Counterclockwise)

Preceding gene: 53720741

Following gene: 53720739

Centisome position: 91.68

GC content: 69.9

Gene sequence:

>774_bases
ATGGCTCTAGCTAAACGCATCATCCCCTGTCTCGACGTGACCGCCGGGCGCGTCGTCAAGGGCGTCAATTTCGTCGAGCT
GCGCGACGCGGGAGACCCCGTCGAGATCGCGCGGCGCTATGACGCCCAGGGCGCGGACGAACTCACGTTCCTCGACATCA
CCGCGACGTCGGACGGCCGCGACCTGATCCTGCCGATCATCGAGGCGGTCGCGTCGCAGGTGTTCATCCCGCTCACGGTG
GGCGGCGGCGTGCGCGCGGTCGAGGACGTGCGGCGCCTCCTGAACGCCGGCGCGGACAAGGTCAGCATGAACTCGTCGGC
GGTCGCCAATCCGCCGCTCGTGCGCGACGCGGCCGATAAGTACGGCTCGCAGTGCATCGTCGTCGCGATCGACGCGAAGC
GCGTGTCGGCCGACGGCGAGCCGCCGCGCTGGGAAGTCTTCACGCACGGCGGCCGCAAGGGCACGGGCCTCGACGCGGTC
GAATGGGCGCGCAAGATGGCCGAGCTCGGCGCGGGCGAGATCCTGCTCACGAGCATGGACCGCGACGGTACGAAGGCGGG
TTTCGATCTCGCGCTCACGCGCGCGGTGTCGGATGCGGTGCCGGTGCCCGTGATCGCGTCGGGCGGCGTCGGCTCGCTCG
AGCATCTCGCGGCGGGCATCACCGAAGGCCACGCGGACGCCGTGCTCGCCGCGAGCATCTTCCATTACGGCGAGCACACG
GTCGGCGAGGCGAAGCGCTTCATGGCCGAGCGTGGCATTGCAGTGAGGTTGTGA

Upstream 100 bases:

>100_bases
GAGCTCGACGACGCCTGACGCGCCGTCTCGGCACGACGCCTTTTCGCCAAGCCGTCCGCCTCGCGCGGGCGGCATTACCC
GCGGCATGCCGCGCAACATC

Downstream 100 bases:

>100_bases
TGAATGCAGAAGCGAAGCCGGGCGACTGGCTGGGCAAGGTCCGCTGGGATGCGAACGGCCTCGTGCCGGTGATCGCGCAG
GACGCGGCGACGAACGACGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAV
EWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
VGEAKRFMAERGIAVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAV
EWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
VGEAKRFMAERGIAVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAVE
WARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHTV
GEAKRFMAERGIAVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.8571428571429, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=32.2314049586777, Blast_Score=105, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=35.4430379746835, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 26979; Mature: 26848

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGR
CCCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHH
YGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAVEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
VGEAKRFMAERGIAVRL
HHHHHHHHHHCCCEEEC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGR
CCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHH
YGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAVEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
VGEAKRFMAERGIAVRL
HHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA