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The map label for this gene is hisF [H]

Identifier: 161723177

GI number: 161723177

Start: 3427631

End: 3428404

Strand: Reverse

Name: hisF [H]

Synonym: BTH_I2987

Alternate gene names: 161723177

Gene position: 3428404-3427631 (Counterclockwise)

Preceding gene: 83719864

Following gene: 83719448

Centisome position: 90.0

GC content: 69.51

Gene sequence:

>774_bases
ATGGCTCTAGCTAAACGCATCATCCCCTGTCTCGACGTGACCGCCGGGCGCGTCGTCAAGGGTGTCAATTTCGTCGAGCT
GCGCGACGCGGGAGACCCCGTCGAGATCGCGCGGCGCTACGACGACCAGGGCGCCGACGAACTCACGTTCCTCGACATCA
CCGCGACGTCGGACGGCCGCGACCTGATCCTGCCGATCATCGAGGCGGTCGCGTCGCAGGTGTTCATCCCGCTCACGGTG
GGCGGCGGCGTGCGCGCGGTCGAAGACGTGCGGCGCCTGCTGAACGCCGGCGCGGACAAGGTCAGCATGAACTCGTCGGC
AGTCGCCAATCCGCAGCTCGTGCGCGACGCGGCCGACAAATACGGCTCGCAGTGCATCGTCGTCGCGATCGACGCGAAGC
GCGTGTCGGCCGAAGGCGAGCCGCCGCGCTGGGAGGTCTTCACGCACGGCGGCCGCAAGGGCACGGGCCTCGACGCGGTC
GAATGGGCGCGCAAGATGGCCGAATTCGGTGCGGGCGAGATCCTGCTGACGAGCATGGACCGCGACGGCACGAAGGCGGG
CTTCGATCTCGCGCTCACGCGCGCGGTATCGGACGCGGTGCCCGTGCCCGTGATCGCGTCGGGCGGGGTCGGCTCGCTCG
AGCATCTCGCGGCGGGCATCACCGAGGGCCACGCCGACGCCGTGCTCGCCGCGAGCATTTTCCACTACGGCGAGCACACG
GTCGGCGAGGCGAAGCGCTACATGGCCGAGCGCGGCATTGCAGTGAGGTTGTGA

Upstream 100 bases:

>100_bases
GAACTCGACGACGCCTGACGCACCGTCTCGGCACGACGCCTTTTCGCCAAGCCGTCCGCCTCGCGCGGCCGGCATTACCC
GCGGCATGCCGCGCAACATC

Downstream 100 bases:

>100_bases
TGAACGAAGCAGCGAAGCCGGGCGGCTGGCTCGACAAGGTCCGCTGGGATGCGAACGGCCTCGTGCCGGTGATCGCGCAG
GACGCGGCGACGAACGACGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSAEGEPPRWEVFTHGGRKGTGLDAV
EWARKMAEFGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
VGEAKRYMAERGIAVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSAEGEPPRWEVFTHGGRKGTGLDAV
EWARKMAEFGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
VGEAKRYMAERGIAVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSAEGEPPRWEVFTHGGRKGTGLDAVE
WARKMAEFGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHTV
GEAKRYMAERGIAVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.8571428571429, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=31.8181818181818, Blast_Score=103, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.6687898089172, Blast_Score=170, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27118; Mature: 26987

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDGR
CCCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSAEGEPPRWEVFTHGGRKGTGLDAVEWARKMAEFGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
VGEAKRYMAERGIAVRL
HHHHHHHHHHCCCEEEC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDGR
CCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSAEGEPPRWEVFTHGGRKGTGLDAVEWARKMAEFGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGITEGHADAVLAASIFHYGEHT
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
VGEAKRYMAERGIAVRL
HHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA