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The map label for this gene is ligA

Identifier: 161621794

GI number: 161621794

Start: 1764008

End: 1766230

Strand: Reverse

Name: ligA

Synonym: PPA1626

Alternate gene names: 161621794

Gene position: 1766230-1764008 (Counterclockwise)

Preceding gene: 50843096

Following gene: 50843092

Centisome position: 68.99

GC content: 60.46

Gene sequence:

>2223_bases
ATGGTTGACGATTCCACCCAAGGTGGCGAGCTGGAGGCGAGCGAGGCTGGGGCCGAAGCGCGCCACCACTGGACTGAGTT
GGCCCAGCGCATCCTTGACGCCCAGGACGCTTACTATGCCCGCGACGCTCCCACGATCTCTGATGCCGAATACGACCGGC
TTATGGTTGAACTCAAGAAGGTGGAGGACGACCATCCGGAGTTGCGCACTCCCGACTCGCCCACTCAGCGGGTTGGAGCT
CCACAACGGATAACAGATTTCGCCCCGGTCAAACACCTCGAACGGCTACTGAGCCTGGACAACGTCTTTACCCGCGACGA
ACTGTCAGAATGGATGAACAGGGTCGCTACTGCCGTCGGAAAGATCCCGAATTTCCTCTGCGAGCTGAAGATTGACGGGT
TGGCGGTCGACCTCGTCTACCGAGACGGGCAGCTTGTATCCGGGGCGACACGCGGTGATGGCCGTATCGGTGAGGACGTC
ACCGCGAACGTGCGTACGATCGCGGCTATACCCCGGAAACTGACTGGCGACGACGTCCCTCGTCTGCTTGAAGTTCGGGG
CGAGGTATTTTTCCCGGTCGCTGATTTCACCGACCTCAACGCTGCCCTCATCGAGGCAGGTAAGAACCCCTTTGCTAACC
CGCGCAATGCGGCTGCCGGTTCGCTGCGGCAGAAGGACTCTCGGGTGACGGCGTCCCGGCCTCTGTCGATGATCGTCCAC
GGTATTGGGGTGCTGGAAGGTCACGATTTCCCTAGCCAGGGGCATGCCTACGACAAGCTTGCGCAGTGGGGGTTACCCGT
CTCGCCGTACTTCAAGATCGTGGAGCATGTCGATGAGGTACATGAGTTCGTGACCCGCTGGGGCGAGTCCCGCGACGAGG
CCTCCCACCAGATTGACGGCGTGGTGGTCAAGGTTGATGATGTCTCTTTGCAGCGCAAGCTGGGTGCCACCTCGCGAGCT
CCCCGGTGGGCCATCGCATATAAATACCCGCCTGAAGAAGTCAACACCGAGCTCCTTGATATCCGCGTCAATGTTGGAAG
GACCGGTCGTGTCACCCCGTACGGGGTCATGAGACCGGTAACAGTTGCCGGGTCCACCGTCGAGATGGCTACCCTCCACA
ACGCTTTCGAGGTCAAGCGCAAAGGAGTGCTCATTGGCGACACCGTGGTGCTGCGCAAGGCTGGTGACGTTATTCCGGAA
ATCCTTGGCCCGGTGGTGGAGCTGCGTAACGGCACTGAGCGGGAGTTTCTTATGCCCGACCACTGCCCGAGCTGTGGGGC
AGAGCTGGCCTACGAAAAGAACGGTGACAAGGATCTTCGCTGCCCTAATGCTCAAGGTTGTCCCTCCCAGCTACACGAGC
GGGTCTTCGGATTAGCGTCACGTGGCGCCCTCGACATCGAAGCTCTTGGTTGGGAGGCCGCCATCGCTCTGACTGATCCG
GAAAACCAGCGCCCGGGTGATGACGAGGTCGCTGAGGAACTTCCGAAACGACAAACGGCAGTGCTGAGCTCCGAGGCTGG
TCTGTTCGACCTGCAGCTCGACGATCTCGCCGAGATCAAAGTATGGCGTCGTAGGAAAGTTAACGGCGGCCCAGGCCCCT
GGCAGCTTGAACCCTATTTCTTCACGAAGGCGACAGCGAAAAAGCCGTCGACTCCGACTGCCACGACCAAGAAAATGTTC
GACGAGTTAGCGAAGGCCAAGTCTCAGCCGTTGTGGAGAGTGCTGGTCGCCCTGTCTATCCGTCATGTCGGACCAACGGC
GGCCCGGGCTCTGGCCACCCACTTCGGGTCAGTTGAGGCCATCCGGGAAGCGTCCGTCGAGGAACTTGCTGGAGTCGATG
GGGTGGGTGAGATTATCGCCGAATCGGTCAAGCGATGGTTCGAAGTCGACTGGCATCAGGAGATTATTAGTCGATGGGCC
GCAGCGGGAGTGCGGATGGCCGACGATCGTGACGAGGCCCCTGAGCAGACCCTCGAGGGGTTGACTGTGGTCGTCACGGG
AAGTCTGGAGGGATTCAGCCGTGACGAGGCGAAGGAGGCCATCGTCTCGCGCGGAGGAAAAGCGGCTGGATCCGTCTCCC
GAAAGACTGACTACGTCGTCGTGGGGGAGAATGCCGGATCCAAGGAAACCAAGGCGCGAGACCTGGGCCGTCCCATCCTT
GACGAGGCCGGGTTCCGGTATTTGCTTGAAAACGGTCCGCAGGGGATCACAACCATCGGGTGA

Upstream 100 bases:

>100_bases
CCAAGTGCATCCCGGTGGCCAGGCAATTCTCTTTGCAACGGACCTGGACGAAAGCTCGTCGGTGAATGCACAACTATGTG
AACTGCGCAAAGATGGGACT

Downstream 100 bases:

>100_bases
GGGTCGATTCTCTGGGGTCTGTCACTCCGATAAGGCAACGTGGACCGGGTAAAGATGCCTGGTCCACGTTGCGTAAGGGT
GCCTCAGAACTCCGAAATGT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MVDDSTQGGELEASEAGAEARHHWTELAQRILDAQDAYYARDAPTISDAEYDRLMVELKKVEDDHPELRTPDSPTQRVGA
PQRITDFAPVKHLERLLSLDNVFTRDELSEWMNRVATAVGKIPNFLCELKIDGLAVDLVYRDGQLVSGATRGDGRIGEDV
TANVRTIAAIPRKLTGDDVPRLLEVRGEVFFPVADFTDLNAALIEAGKNPFANPRNAAAGSLRQKDSRVTASRPLSMIVH
GIGVLEGHDFPSQGHAYDKLAQWGLPVSPYFKIVEHVDEVHEFVTRWGESRDEASHQIDGVVVKVDDVSLQRKLGATSRA
PRWAIAYKYPPEEVNTELLDIRVNVGRTGRVTPYGVMRPVTVAGSTVEMATLHNAFEVKRKGVLIGDTVVLRKAGDVIPE
ILGPVVELRNGTEREFLMPDHCPSCGAELAYEKNGDKDLRCPNAQGCPSQLHERVFGLASRGALDIEALGWEAAIALTDP
ENQRPGDDEVAEELPKRQTAVLSSEAGLFDLQLDDLAEIKVWRRRKVNGGPGPWQLEPYFFTKATAKKPSTPTATTKKMF
DELAKAKSQPLWRVLVALSIRHVGPTAARALATHFGSVEAIREASVEELAGVDGVGEIIAESVKRWFEVDWHQEIISRWA
AAGVRMADDRDEAPEQTLEGLTVVVTGSLEGFSRDEAKEAIVSRGGKAAGSVSRKTDYVVVGENAGSKETKARDLGRPIL
DEAGFRYLLENGPQGITTIG

Sequences:

>Translated_740_residues
MVDDSTQGGELEASEAGAEARHHWTELAQRILDAQDAYYARDAPTISDAEYDRLMVELKKVEDDHPELRTPDSPTQRVGA
PQRITDFAPVKHLERLLSLDNVFTRDELSEWMNRVATAVGKIPNFLCELKIDGLAVDLVYRDGQLVSGATRGDGRIGEDV
TANVRTIAAIPRKLTGDDVPRLLEVRGEVFFPVADFTDLNAALIEAGKNPFANPRNAAAGSLRQKDSRVTASRPLSMIVH
GIGVLEGHDFPSQGHAYDKLAQWGLPVSPYFKIVEHVDEVHEFVTRWGESRDEASHQIDGVVVKVDDVSLQRKLGATSRA
PRWAIAYKYPPEEVNTELLDIRVNVGRTGRVTPYGVMRPVTVAGSTVEMATLHNAFEVKRKGVLIGDTVVLRKAGDVIPE
ILGPVVELRNGTEREFLMPDHCPSCGAELAYEKNGDKDLRCPNAQGCPSQLHERVFGLASRGALDIEALGWEAAIALTDP
ENQRPGDDEVAEELPKRQTAVLSSEAGLFDLQLDDLAEIKVWRRRKVNGGPGPWQLEPYFFTKATAKKPSTPTATTKKMF
DELAKAKSQPLWRVLVALSIRHVGPTAARALATHFGSVEAIREASVEELAGVDGVGEIIAESVKRWFEVDWHQEIISRWA
AAGVRMADDRDEAPEQTLEGLTVVVTGSLEGFSRDEAKEAIVSRGGKAAGSVSRKTDYVVVGENAGSKETKARDLGRPIL
DEAGFRYLLENGPQGITTIG
>Mature_740_residues
MVDDSTQGGELEASEAGAEARHHWTELAQRILDAQDAYYARDAPTISDAEYDRLMVELKKVEDDHPELRTPDSPTQRVGA
PQRITDFAPVKHLERLLSLDNVFTRDELSEWMNRVATAVGKIPNFLCELKIDGLAVDLVYRDGQLVSGATRGDGRIGEDV
TANVRTIAAIPRKLTGDDVPRLLEVRGEVFFPVADFTDLNAALIEAGKNPFANPRNAAAGSLRQKDSRVTASRPLSMIVH
GIGVLEGHDFPSQGHAYDKLAQWGLPVSPYFKIVEHVDEVHEFVTRWGESRDEASHQIDGVVVKVDDVSLQRKLGATSRA
PRWAIAYKYPPEEVNTELLDIRVNVGRTGRVTPYGVMRPVTVAGSTVEMATLHNAFEVKRKGVLIGDTVVLRKAGDVIPE
ILGPVVELRNGTEREFLMPDHCPSCGAELAYEKNGDKDLRCPNAQGCPSQLHERVFGLASRGALDIEALGWEAAIALTDP
ENQRPGDDEVAEELPKRQTAVLSSEAGLFDLQLDDLAEIKVWRRRKVNGGPGPWQLEPYFFTKATAKKPSTPTATTKKMF
DELAKAKSQPLWRVLVALSIRHVGPTAARALATHFGSVEAIREASVEELAGVDGVGEIIAESVKRWFEVDWHQEIISRWA
AAGVRMADDRDEAPEQTLEGLTVVVTGSLEGFSRDEAKEAIVSRGGKAAGSVSRKTDYVVVGENAGSKETKARDLGRPIL
DEAGFRYLLENGPQGITTIG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=80, Percent_Identity=50, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1788750, Length=714, Percent_Identity=41.5966386554622, Blast_Score=497, Evalue=1e-141,
Organism=Escherichia coli, GI87082305, Length=457, Percent_Identity=24.945295404814, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PROAC (Q6A7A2)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056321.2
- HSSP:   P63973
- ProteinModelPortal:   Q6A7A2
- GeneID:   2932781
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1626
- NMPDR:   fig|267747.1.peg.1598
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   PACN267747:PPA1626-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 81063; Mature: 81063

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 162-162 BINDING 160-160 BINDING 183-183 BINDING 220-220 BINDING 336-336 BINDING 360-360

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDDSTQGGELEASEAGAEARHHWTELAQRILDAQDAYYARDAPTISDAEYDRLMVELKK
CCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VEDDHPELRTPDSPTQRVGAPQRITDFAPVKHLERLLSLDNVFTRDELSEWMNRVATAVG
HCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIPNFLCELKIDGLAVDLVYRDGQLVSGATRGDGRIGEDVTANVRTIAAIPRKLTGDDVP
HHHHHEEEEEECCEEEEEEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHH
RLLEVRGEVFFPVADFTDLNAALIEAGKNPFANPRNAAAGSLRQKDSRVTASRPLSMIVH
HHHHHCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHCCHHHHCCCCCHHCCHHHHHH
GIGVLEGHDFPSQGHAYDKLAQWGLPVSPYFKIVEHVDEVHEFVTRWGESRDEASHQIDG
HHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
VVVKVDDVSLQRKLGATSRAPRWAIAYKYPPEEVNTELLDIRVNVGRTGRVTPYGVMRPV
EEEEECCHHHHHHHCCCCCCCCEEEEEECCHHHHCCEEEEEEEECCCCCCCCCCCCCCCE
TVAGSTVEMATLHNAFEVKRKGVLIGDTVVLRKAGDVIPEILGPVVELRNGTEREFLMPD
EECCCCCHHHHHHHHHHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HCPSCGAELAYEKNGDKDLRCPNAQGCPSQLHERVFGLASRGALDIEALGWEAAIALTDP
CCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECC
ENQRPGDDEVAEELPKRQTAVLSSEAGLFDLQLDDLAEIKVWRRRKVNGGPGPWQLEPYF
CCCCCCCHHHHHHCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FTKATAKKPSTPTATTKKMFDELAKAKSQPLWRVLVALSIRHVGPTAARALATHFGSVEA
EECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHH
IREASVEELAGVDGVGEIIAESVKRWFEVDWHQEIISRWAAAGVRMADDRDEAPEQTLEG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC
LTVVVTGSLEGFSRDEAKEAIVSRGGKAAGSVSRKTDYVVVGENAGSKETKARDLGRPIL
EEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCHHH
DEAGFRYLLENGPQGITTIG
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVDDSTQGGELEASEAGAEARHHWTELAQRILDAQDAYYARDAPTISDAEYDRLMVELKK
CCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VEDDHPELRTPDSPTQRVGAPQRITDFAPVKHLERLLSLDNVFTRDELSEWMNRVATAVG
HCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIPNFLCELKIDGLAVDLVYRDGQLVSGATRGDGRIGEDVTANVRTIAAIPRKLTGDDVP
HHHHHEEEEEECCEEEEEEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHH
RLLEVRGEVFFPVADFTDLNAALIEAGKNPFANPRNAAAGSLRQKDSRVTASRPLSMIVH
HHHHHCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHCCHHHHCCCCCHHCCHHHHHH
GIGVLEGHDFPSQGHAYDKLAQWGLPVSPYFKIVEHVDEVHEFVTRWGESRDEASHQIDG
HHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
VVVKVDDVSLQRKLGATSRAPRWAIAYKYPPEEVNTELLDIRVNVGRTGRVTPYGVMRPV
EEEEECCHHHHHHHCCCCCCCCEEEEEECCHHHHCCEEEEEEEECCCCCCCCCCCCCCCE
TVAGSTVEMATLHNAFEVKRKGVLIGDTVVLRKAGDVIPEILGPVVELRNGTEREFLMPD
EECCCCCHHHHHHHHHHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HCPSCGAELAYEKNGDKDLRCPNAQGCPSQLHERVFGLASRGALDIEALGWEAAIALTDP
CCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECC
ENQRPGDDEVAEELPKRQTAVLSSEAGLFDLQLDDLAEIKVWRRRKVNGGPGPWQLEPYF
CCCCCCCHHHHHHCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FTKATAKKPSTPTATTKKMFDELAKAKSQPLWRVLVALSIRHVGPTAARALATHFGSVEA
EECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHH
IREASVEELAGVDGVGEIIAESVKRWFEVDWHQEIISRWAAAGVRMADDRDEAPEQTLEG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC
LTVVVTGSLEGFSRDEAKEAIVSRGGKAAGSVSRKTDYVVVGENAGSKETKARDLGRPIL
EEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCHHH
DEAGFRYLLENGPQGITTIG
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA