Click here to switch to the map view.

The map label for this gene is sbcB [H]

Identifier: 161621731

GI number: 161621731

Start: 3369107

End: 3370540

Strand: Reverse

Name: sbcB [H]

Synonym: RSc3125

Alternate gene names: 161621731

Gene position: 3370540-3369107 (Counterclockwise)

Preceding gene: 17547845

Following gene: 17547842

Centisome position: 90.69

GC content: 67.43

Gene sequence:

>1434_bases
GTGACCCACACCTTCTTCTGGCACGACTACGAAACCTTCGGCGCCGTGCCGCGCCGCGATCGCCCGGCCCAGTTCGCCGG
CATCCGTACCGACGCCGAGCTCAACGAGATCGGCGAACCGGTCGAGCTGTTCTGTCAGCCGTCCAACGACTGGCTGCCCG
ACCCGATGTCGTGCCTGATCACCGGCATCACGCCGCAGCAGTGCCGCCGGCAGGGTATCCCCGAGCACCGTTTCGCGCAG
GCCATCGAGCGCGAGCTGGCCACGCCCGGCACCATCGGCGTCGGCTATAACTCCATCCGCTTCGACGATGAGATCACGCG
CCACCTGTTCTGGCGCAACCTGATCGATCCGTATGCGCGCGAATGGCAGAACACGTGCGGCCGATGGGATCTGCTCGATG
TGGTGCGCACCACCTGGGCGCTGCGCCCGGACGGCGTCGAATGGCCGAAGAACGGCGACGGCAAGCCCAGCTTCAAGCTG
GAGCACCTGTCGCAGGCCAACGGCCTGCAGCACGAAGCCGCACACGATGCGCTGTCGGATGTGCGGGCCACCATCGCGCT
GGCCCGGCGGGTCCGCGCCGCGCAGCCCCGCCTGTTCGATTTCTGCTTCGCGCTGCGCAAGAAGGAGCGCGTGCTGGCCG
AGATCGGCGACGCGTCGCGGCCGTTGCTGCACGTCTCGGGCATGTACGGCGTGGAGCGGGGCTGCCTGGCGGTAGTGTGG
CCGCTGGGCTGGCACCCGACCAACAAGAACGAACTGCTGGTGTGGGACCTCGCCTGCGATCCGGCCGAGCTGTTCGACCT
CGGCGTTGACACCATCCGCGAGCGCCTGTTCACGCGCAGCGCCGATCTGACCGAGGGCACCACGCGCTTGCCCATCAAGT
CGATCCACATCAACAAGTCGCCCATCGTCATCGGCAACCTGAAGACGCTGCAGCCCGCGCAGGCCGAGCGCTGGGGCATC
GACTTCGCGGTCATCGAGCGTCATGCCGGTATCGCCCAAGGGGCGCCCGATCTGCGCGAAACGTGGCGCCAGGTCTACGC
CCGCGAGCTGGAGCCGATCGAAGACGTGGACCAGAATCTCTACGGCGGCTTCGTCGGCAATGACGACCGCCGCACGCTCA
ACGAACTGCGCACGCTTTCGGGCGAGCAGCTCGCGCAGCTGCACCCGGACTTCGCCGATGCGCGCCTGCCCGAACTGCTG
TTCCGCTATCGCGCGCGCAATTTTCCCGACACGCTCACGGAAGAGGAGTACGAACAGTGGGAGCAGTTGCGCGCCGAGCG
CCTGTTCGAGGGGCGCGAGGGCTACCTGACCTTCGATGCCTTCGGCGAGCGGATCGAGCAGCTTGCCGCCGAGGCCGCCG
AGCGTGACGACGCGCGCGCGCAGAATGTGCTGCAGGATCTGTACGACTACGCTCAGCAGATTCTGCCGGGCTGA

Upstream 100 bases:

>100_bases
TGCTGGAGGCCGGCCGGCGCAACTTCCTGCTCGATTGACGGGCGCGACCGGCAGGGCTGTTAGCATGCCGGCATCTTTGC
GATGCCCAACCCGCCTGACC

Downstream 100 bases:

>100_bases
GGCAACGCTGGTGGCACTCGGGTGGTCCGTCGCCGATCATTCGTCCCGACACGGGGTCGCGTCGCGGTCGCGTGCCGGTC
CAACGTCTGCTGCGAAGGCG

Product: exonuclease I

Products: NA

Alternate protein names: Exonuclease I; DNA deoxyribophosphodiesterase; dRPase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTHTFFWHDYETFGAVPRRDRPAQFAGIRTDAELNEIGEPVELFCQPSNDWLPDPMSCLITGITPQQCRRQGIPEHRFAQ
AIERELATPGTIGVGYNSIRFDDEITRHLFWRNLIDPYAREWQNTCGRWDLLDVVRTTWALRPDGVEWPKNGDGKPSFKL
EHLSQANGLQHEAAHDALSDVRATIALARRVRAAQPRLFDFCFALRKKERVLAEIGDASRPLLHVSGMYGVERGCLAVVW
PLGWHPTNKNELLVWDLACDPAELFDLGVDTIRERLFTRSADLTEGTTRLPIKSIHINKSPIVIGNLKTLQPAQAERWGI
DFAVIERHAGIAQGAPDLRETWRQVYARELEPIEDVDQNLYGGFVGNDDRRTLNELRTLSGEQLAQLHPDFADARLPELL
FRYRARNFPDTLTEEEYEQWEQLRAERLFEGREGYLTFDAFGERIEQLAAEAAERDDARAQNVLQDLYDYAQQILPG

Sequences:

>Translated_477_residues
MTHTFFWHDYETFGAVPRRDRPAQFAGIRTDAELNEIGEPVELFCQPSNDWLPDPMSCLITGITPQQCRRQGIPEHRFAQ
AIERELATPGTIGVGYNSIRFDDEITRHLFWRNLIDPYAREWQNTCGRWDLLDVVRTTWALRPDGVEWPKNGDGKPSFKL
EHLSQANGLQHEAAHDALSDVRATIALARRVRAAQPRLFDFCFALRKKERVLAEIGDASRPLLHVSGMYGVERGCLAVVW
PLGWHPTNKNELLVWDLACDPAELFDLGVDTIRERLFTRSADLTEGTTRLPIKSIHINKSPIVIGNLKTLQPAQAERWGI
DFAVIERHAGIAQGAPDLRETWRQVYARELEPIEDVDQNLYGGFVGNDDRRTLNELRTLSGEQLAQLHPDFADARLPELL
FRYRARNFPDTLTEEEYEQWEQLRAERLFEGREGYLTFDAFGERIEQLAAEAAERDDARAQNVLQDLYDYAQQILPG
>Mature_476_residues
THTFFWHDYETFGAVPRRDRPAQFAGIRTDAELNEIGEPVELFCQPSNDWLPDPMSCLITGITPQQCRRQGIPEHRFAQA
IERELATPGTIGVGYNSIRFDDEITRHLFWRNLIDPYAREWQNTCGRWDLLDVVRTTWALRPDGVEWPKNGDGKPSFKLE
HLSQANGLQHEAAHDALSDVRATIALARRVRAAQPRLFDFCFALRKKERVLAEIGDASRPLLHVSGMYGVERGCLAVVWP
LGWHPTNKNELLVWDLACDPAELFDLGVDTIRERLFTRSADLTEGTTRLPIKSIHINKSPIVIGNLKTLQPAQAERWGID
FAVIERHAGIAQGAPDLRETWRQVYARELEPIEDVDQNLYGGFVGNDDRRTLNELRTLSGEQLAQLHPDFADARLPELLF
RYRARNFPDTLTEEEYEQWEQLRAERLFEGREGYLTFDAFGERIEQLAAEAAERDDARAQNVLQDLYDYAQQILPG

Specific function: Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase [H]

COG id: COG2925

COG function: function code L; Exonuclease I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788321, Length=476, Percent_Identity=44.9579831932773, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013620
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T; PF08411 Exonuc_X-T_C [H]

EC number: =3.1.11.1 [H]

Molecular weight: Translated: 54683; Mature: 54552

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHTFFWHDYETFGAVPRRDRPAQFAGIRTDAELNEIGEPVELFCQPSNDWLPDPMSCLI
CCCEEEECCHHHHCCCCCCCCCCHHCCCCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHE
TGITPQQCRRQGIPEHRFAQAIERELATPGTIGVGYNSIRFDDEITRHLFWRNLIDPYAR
ECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCEECHHHHHHHHHHHHHHHHHHH
EWQNTCGRWDLLDVVRTTWALRPDGVEWPKNGDGKPSFKLEHLSQANGLQHEAAHDALSD
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
VRATIALARRVRAAQPRLFDFCFALRKKERVLAEIGDASRPLLHVSGMYGVERGCLAVVW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEE
PLGWHPTNKNELLVWDLACDPAELFDLGVDTIRERLFTRSADLTEGTTRLPIKSIHINKS
CCCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
PIVIGNLKTLQPAQAERWGIDFAVIERHAGIAQGAPDLRETWRQVYARELEPIEDVDQNL
CEEEECCCCCCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
YGGFVGNDDRRTLNELRTLSGEQLAQLHPDFADARLPELLFRYRARNFPDTLTEEEYEQW
CCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EQLRAERLFEGREGYLTFDAFGERIEQLAAEAAERDDARAQNVLQDLYDYAQQILPG
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THTFFWHDYETFGAVPRRDRPAQFAGIRTDAELNEIGEPVELFCQPSNDWLPDPMSCLI
CCEEEECCHHHHCCCCCCCCCCHHCCCCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHE
TGITPQQCRRQGIPEHRFAQAIERELATPGTIGVGYNSIRFDDEITRHLFWRNLIDPYAR
ECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCEECHHHHHHHHHHHHHHHHHHH
EWQNTCGRWDLLDVVRTTWALRPDGVEWPKNGDGKPSFKLEHLSQANGLQHEAAHDALSD
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
VRATIALARRVRAAQPRLFDFCFALRKKERVLAEIGDASRPLLHVSGMYGVERGCLAVVW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEE
PLGWHPTNKNELLVWDLACDPAELFDLGVDTIRERLFTRSADLTEGTTRLPIKSIHINKS
CCCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
PIVIGNLKTLQPAQAERWGIDFAVIERHAGIAQGAPDLRETWRQVYARELEPIEDVDQNL
CEEEECCCCCCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
YGGFVGNDDRRTLNELRTLSGEQLAQLHPDFADARLPELLFRYRARNFPDTLTEEEYEQW
CCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EQLRAERLFEGREGYLTFDAFGERIEQLAAEAAERDDARAQNVLQDLYDYAQQILPG
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3539937; 9097040; 9278503; 1329027 [H]