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The map label for this gene is pheS [H]

Identifier: 161621316

GI number: 161621316

Start: 3398232

End: 3399254

Strand: Reverse

Name: pheS [H]

Synonym: Bxe_A1375

Alternate gene names: 161621316

Gene position: 3399254-3398232 (Counterclockwise)

Preceding gene: 91784425

Following gene: 91784423

Centisome position: 69.43

GC content: 61.39

Gene sequence:

>1023_bases
ATGGGATCAATGGATCTGGACCAGATTGTCGCCGACGCGCAAAAAGCCTTCGCAGAAGCCACCGACGTCACCACCCTCGA
GAACGAGAAAGCTCGCTTTCTCGGCAAATCGGGTGCGCTGACCGAGCTGTTGAAGGGCCTTGGCAAACTCGATCCCGAAA
CGCGCAAGACCGAAGGCGCTCGGATCAACCTCGTCAAGCAACAAGTGGAAGCCGCGTTGACGGCCCGCCGTCAGGCGTTG
GCCGAGGCGTTGCTGAACCAGCGCCTCGCCGCTGAAGCCATCGACGTCACGCTGCCCGGCCGCGGCACCGGCGCAGGCAG
CCTGCACCCGGTAATGCGCACGTGGGAGCGCGTCGAACAGATTTTCCGTACGATCGGATTCGACGTGGCCGACGGCCCCG
AAATCGAAACCGACTGGTACAACTTCACCTCGTTGAACAGCCCGGAGAATCATCCGGCGCGGTCCATGCAGGACACTTTT
TACGTCGACGGCAAAGATGCCGACGGCCGCCAGCTGCTGCTGCGCACGCACACCAGCCCGATGCAGGTTCGCTACGCGCG
CACCAACACGCCGCCCATCAAGGTGATCGTGCCGGGCCGCACGTACCGTGTGGACAGCGACGCAACTCACTCGCCCATGT
TCAACCAGGTCGAAGGCCTGTGGATCGACGAGAACATCAGCTTCGCGGACCTGAAGGGCGTCTACACCGACTTCCTCGAG
AAATTCTTCGAGCGCGACGATATTCAGGTGCGCTTCCGTCCGTCGTACTTTCCGTTCACCGAACCGTCGGCGGAGATCGA
CATGTTGTTCGAAACGGGCAAGAACGCCGGCAAGTGGCTCGAAATTTCGGGCTCGGGCCAGGTTCACCCCACGGTGATCC
GCAATATGGGCCTCGACCCGGAGCGTTACATCGGCTTTGCTTTCGGCAGTGGCCTCGAGCGGCTCACCATGTTGCGTTAC
GGCGTGCAGGACCTGCGTCTGTTCTTCGAAAACGACCTGCGTTTCCTGCGTCAATTCGCGTGA

Upstream 100 bases:

>100_bases
GTAGTCCCGGTGACGCTGCAGCAAAAACGGGGCTCCTCACCGAGCCCCGTTTTTGTTGGTAGAACCAGTTTTGCATCGAT
TGAACACTGACGTTGAAATG

Downstream 100 bases:

>100_bases
ACCGGCGCGTACGAACGCGACGAGCATAGAGCGCGGCAACGCACGCAGTGCATGCCGCCACAGCGTCCCCGGTAGTGTCC
GATGATTCGCACCGCATGAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MGSMDLDQIVADAQKAFAEATDVTTLENEKARFLGKSGALTELLKGLGKLDPETRKTEGARINLVKQQVEAALTARRQAL
AEALLNQRLAAEAIDVTLPGRGTGAGSLHPVMRTWERVEQIFRTIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTF
YVDGKDADGRQLLLRTHTSPMQVRYARTNTPPIKVIVPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLE
KFFERDDIQVRFRPSYFPFTEPSAEIDMLFETGKNAGKWLEISGSGQVHPTVIRNMGLDPERYIGFAFGSGLERLTMLRY
GVQDLRLFFENDLRFLRQFA

Sequences:

>Translated_340_residues
MGSMDLDQIVADAQKAFAEATDVTTLENEKARFLGKSGALTELLKGLGKLDPETRKTEGARINLVKQQVEAALTARRQAL
AEALLNQRLAAEAIDVTLPGRGTGAGSLHPVMRTWERVEQIFRTIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTF
YVDGKDADGRQLLLRTHTSPMQVRYARTNTPPIKVIVPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLE
KFFERDDIQVRFRPSYFPFTEPSAEIDMLFETGKNAGKWLEISGSGQVHPTVIRNMGLDPERYIGFAFGSGLERLTMLRY
GVQDLRLFFENDLRFLRQFA
>Mature_339_residues
GSMDLDQIVADAQKAFAEATDVTTLENEKARFLGKSGALTELLKGLGKLDPETRKTEGARINLVKQQVEAALTARRQALA
EALLNQRLAAEAIDVTLPGRGTGAGSLHPVMRTWERVEQIFRTIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTFY
VDGKDADGRQLLLRTHTSPMQVRYARTNTPPIKVIVPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLEK
FFERDDIQVRFRPSYFPFTEPSAEIDMLFETGKNAGKWLEISGSGQVHPTVIRNMGLDPERYIGFAFGSGLERLTMLRYG
VQDLRLFFENDLRFLRQFA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=262, Percent_Identity=31.2977099236641, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI5729820, Length=290, Percent_Identity=31.0344827586207, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788007, Length=334, Percent_Identity=57.4850299401198, Blast_Score=383, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=32.4528301886792, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32563657, Length=264, Percent_Identity=32.5757575757576, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32566635, Length=275, Percent_Identity=31.6363636363636, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325304, Length=94, Percent_Identity=40.4255319148936, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18858079, Length=300, Percent_Identity=31, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137424, Length=248, Percent_Identity=29.8387096774194, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38367; Mature: 38236

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSMDLDQIVADAQKAFAEATDVTTLENEKARFLGKSGALTELLKGLGKLDPETRKTEGA
CCCCCHHHHHHHHHHHHHHHHCCEECCCCHHHHHCCCCCHHHHHHHHHCCCCCHHCCCCC
RINLVKQQVEAALTARRQALAEALLNQRLAAEAIDVTLPGRGTGAGSLHPVMRTWERVEQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHH
IFRTIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTFYVDGKDADGRQLLLRTHTSP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
MQVRYARTNTPPIKVIVPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLE
EEEEEEECCCCCEEEEECCCEEEECCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHH
KFFERDDIQVRFRPSYFPFTEPSAEIDMLFETGKNAGKWLEISGSGQVHPTVIRNMGLDP
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCH
ERYIGFAFGSGLERLTMLRYGVQDLRLFFENDLRFLRQFA
HHHEEEHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GSMDLDQIVADAQKAFAEATDVTTLENEKARFLGKSGALTELLKGLGKLDPETRKTEGA
CCCCHHHHHHHHHHHHHHHHCCEECCCCHHHHHCCCCCHHHHHHHHHCCCCCHHCCCCC
RINLVKQQVEAALTARRQALAEALLNQRLAAEAIDVTLPGRGTGAGSLHPVMRTWERVEQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHH
IFRTIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTFYVDGKDADGRQLLLRTHTSP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
MQVRYARTNTPPIKVIVPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLE
EEEEEEECCCCCEEEEECCCEEEECCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHH
KFFERDDIQVRFRPSYFPFTEPSAEIDMLFETGKNAGKWLEISGSGQVHPTVIRNMGLDP
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCH
ERYIGFAFGSGLERLTMLRYGVQDLRLFFENDLRFLRQFA
HHHEEEHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA