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The map label for this gene is leuA

Identifier: 161611210

GI number: 161611210

Start: 333939

End: 335633

Strand: Direct

Name: leuA

Synonym: MAP0312

Alternate gene names: 161611210

Gene position: 333939-335633 (Clockwise)

Preceding gene: 41406399

Following gene: 41406412

Centisome position: 6.91

GC content: 68.14

Gene sequence:

>1695_bases
ATGCCGGTCAACCGGTACCGGCCGTTCGCCGAGGAGGTCGAGCCCATCCGGCTGCCCGACCGCACCTGGCCCGACAAGGT
CATCGACCGTGCCCCGCTGTGGTGCGCGGTGGACCTGCGCGACGGCAACCAGGCGCTGATCGACCCGATGAGCCCGGCCC
GCAAGCGCCGCATGTTCGACCTGCTGGTCCGCATGGGCTACAAGGAGATCGAGGTCGGCTTCCCGTCGGCCAGCCAGACC
GATTTCGACTTCGTCCGCGAGATCATCACCGAGGGCGCCATTCCCGACGACGTCACCATTCAGGTGCTGACCCAGTGCCG
GCCGGAACTGATCGAGCGCACCTTCGCCGCGTGCCAGGGCGCGCCGCAGGCCATCGTGCACTTCTACAACTCGACGTCAA
TCCTGCAGCGGCGCGTGGTATTTCGCGCCGACCGGGCCGCGGTGCAGGAGATCGCCGTGGCCGGCGCCCGCAAGTGCGTC
GAGGAGGCCGCCAAGTACCCCGAGACGCAGTGGCGGTTCGAGTACTCGCCGGAGTCCTACACCGGAACCGAACTGGAGTA
CGCCAAGCAGGTGTGCGACGCGGTCGGCGAAATCATAAGGCCCACAACGGAAAACCCGATCATCTTCAACCTGCCCGCCA
CGGTGGAGATGGCCACCCCCAACGTGTACGCCGACTCCATCGAGTGGATGAGCCGCAATCTGGCCCGCCGCGACTCCGTC
ATCCTGAGCCTGCACCCGCACAACGACCGCGGAACCGCCGTCGCCGCAGCGGAATTGGGCTATGCGGCCGGCGCCGACCG
GATCGAGGGCTGCCTGTTCGGCAACGGCGAACGCACCGGCAACGTGTGCCTGGTGACGCTGGGGCTGAACCTGTTCTCCC
GCGGCGTGGACCCGCAGATCGACTTCTCCAACATCGACGAGATCCGCCGCACGGTGGAGTACTGCAATCAGCTGCCGGTG
CACGAGCGGCACCCCTACGGCGGCGACCTGGTCTACACCGCGTTCTCCGGCAGCCACCAGGACGCCATCAACAAGGGCCT
GGACCAGATGAAGATCGACGCCGACGCCGCCGACACCGACGTCGAGGACATGCTCTGGCAGGTGCCGTATCTGCCGATCG
ACCCCAAGGACGTCGGGCGCACCTACGAGGCGGTGATCCGGGTGAACTCGCAGTCCGGCAAGGGCGGGGTGGCCTACATC
ATGAAGGCCGATCACGGCCTGGCCCTGCCGCGCCGGCTGCAGATCGAGTTCTCCCAGGTGATCCAGAAGATCAGCGAGGG
CGAGGGCGGCGAGGTCAGCCCCAAGGAGATGTGGGACGCGTTCTTCGAGGAGTACCTGGCCCCGGTGTGGCCGCTGGAGC
GGGTCAAGCAGCGCGTCGACGCCTCCGACGAGGACAGCGGCGTCACCAGCATCTCCGCGACGGTCAAGATCAACGGGGAG
CAGACCGAGATCAACGGGACCGGCAACGGCCCGCTGGCCGCGTTCGTGCACGCGCTCGCCGGGGTCGGGTTCGACGTGGC
GGTGCTGGACTACTCCGAGCACGCCATGAGTTCGGGCGACGACGCGCAGGCCGCCGCGTATGTGGAGGCGTCGGTCAACG
GTCGGACGGTGTGGGGTGTCGGCATCGCCCCGTCCATCACCACCGCCTCGTTGCGCGCGGTGGTGTCGGCGGTCAACCGC
GCCGCCCGCGCCTGA

Upstream 100 bases:

>100_bases
TTCCCATCCCGACGCCTTCAGCTCGTCCCGCAGCATCGTCAAACCCGCCGGCCCGCCCGGCCCCGGACAGCCCGCCTGGA
ACCCCCAGCGCGGCTCCTCG

Downstream 100 bases:

>100_bases
TCAGAACAACGCCAGCTGCGGATCGGGCGTGGCGTCGGTGAACTCGTCCATGCTGGTGCCGCCGATGACGGCGCGAACGC
AGTCCAAAAACTGCGCGTCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MPVNRYRPFAEEVEPIRLPDRTWPDKVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQT
DFDFVREIITEGAIPDDVTIQVLTQCRPELIERTFAACQGAPQAIVHFYNSTSILQRRVVFRADRAAVQEIAVAGARKCV
EEAAKYPETQWRFEYSPESYTGTELEYAKQVCDAVGEIIRPTTENPIIFNLPATVEMATPNVYADSIEWMSRNLARRDSV
ILSLHPHNDRGTAVAAAELGYAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPV
HERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADTDVEDMLWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYI
MKADHGLALPRRLQIEFSQVIQKISEGEGGEVSPKEMWDAFFEEYLAPVWPLERVKQRVDASDEDSGVTSISATVKINGE
QTEINGTGNGPLAAFVHALAGVGFDVAVLDYSEHAMSSGDDAQAAAYVEASVNGRTVWGVGIAPSITTASLRAVVSAVNR
AARA

Sequences:

>Translated_564_residues
MPVNRYRPFAEEVEPIRLPDRTWPDKVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQT
DFDFVREIITEGAIPDDVTIQVLTQCRPELIERTFAACQGAPQAIVHFYNSTSILQRRVVFRADRAAVQEIAVAGARKCV
EEAAKYPETQWRFEYSPESYTGTELEYAKQVCDAVGEIIRPTTENPIIFNLPATVEMATPNVYADSIEWMSRNLARRDSV
ILSLHPHNDRGTAVAAAELGYAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPV
HERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADTDVEDMLWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYI
MKADHGLALPRRLQIEFSQVIQKISEGEGGEVSPKEMWDAFFEEYLAPVWPLERVKQRVDASDEDSGVTSISATVKINGE
QTEINGTGNGPLAAFVHALAGVGFDVAVLDYSEHAMSSGDDAQAAAYVEASVNGRTVWGVGIAPSITTASLRAVVSAVNR
AARA
>Mature_563_residues
PVNRYRPFAEEVEPIRLPDRTWPDKVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTD
FDFVREIITEGAIPDDVTIQVLTQCRPELIERTFAACQGAPQAIVHFYNSTSILQRRVVFRADRAAVQEIAVAGARKCVE
EAAKYPETQWRFEYSPESYTGTELEYAKQVCDAVGEIIRPTTENPIIFNLPATVEMATPNVYADSIEWMSRNLARRDSVI
LSLHPHNDRGTAVAAAELGYAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVH
ERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADTDVEDMLWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIM
KADHGLALPRRLQIEFSQVIQKISEGEGGEVSPKEMWDAFFEEYLAPVWPLERVKQRVDASDEDSGVTSISATVKINGEQ
TEINGTGNGPLAAFVHALAGVGFDVAVLDYSEHAMSSGDDAQAAAYVEASVNGRTVWGVGIAPSITTASLRAVVSAVNRA
ARA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=536, Percent_Identity=25.9328358208955, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=570, Percent_Identity=46.140350877193, Blast_Score=510, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6324682, Length=576, Percent_Identity=45.8333333333333, Blast_Score=501, Evalue=1e-142,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62091; Mature: 61960

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVNRYRPFAEEVEPIRLPDRTWPDKVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFD
CCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVRMGYKEIEVGFPSASQTDFDFVREIITEGAIPDDVTIQVLTQCRPELIERTFAACQG
HHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
APQAIVHFYNSTSILQRRVVFRADRAAVQEIAVAGARKCVEEAAKYPETQWRFEYSPESY
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
TGTELEYAKQVCDAVGEIIRPTTENPIIFNLPATVEMATPNVYADSIEWMSRNLARRDSV
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCE
ILSLHPHNDRGTAVAAAELGYAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQI
EEEEECCCCCCCEEEHHHHCCCCCCHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCC
DFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADTD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHCCHHHEEECCCCCCCC
VEDMLWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLALPRRLQIEFSQV
HHHHHHHCCCCCCCHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
IQKISEGEGGEVSPKEMWDAFFEEYLAPVWPLERVKQRVDASDEDSGVTSISATVKINGE
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEECCC
QTEINGTGNGPLAAFVHALAGVGFDVAVLDYSEHAMSSGDDAQAAAYVEASVNGRTVWGV
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCCCEEEEEEECCCCEEEEEE
GIAPSITTASLRAVVSAVNRAARA
CCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVNRYRPFAEEVEPIRLPDRTWPDKVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFD
CCCCCCCHHHCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVRMGYKEIEVGFPSASQTDFDFVREIITEGAIPDDVTIQVLTQCRPELIERTFAACQG
HHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
APQAIVHFYNSTSILQRRVVFRADRAAVQEIAVAGARKCVEEAAKYPETQWRFEYSPESY
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
TGTELEYAKQVCDAVGEIIRPTTENPIIFNLPATVEMATPNVYADSIEWMSRNLARRDSV
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCE
ILSLHPHNDRGTAVAAAELGYAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQI
EEEEECCCCCCCEEEHHHHCCCCCCHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCC
DFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADTD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHCCHHHEEECCCCCCCC
VEDMLWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLALPRRLQIEFSQV
HHHHHHHCCCCCCCHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
IQKISEGEGGEVSPKEMWDAFFEEYLAPVWPLERVKQRVDASDEDSGVTSISATVKINGE
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEEEEEECCC
QTEINGTGNGPLAAFVHALAGVGFDVAVLDYSEHAMSSGDDAQAAAYVEASVNGRTVWGV
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCCCEEEEEEECCCCEEEEEE
GIAPSITTASLRAVVSAVNRAARA
CCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA