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The map label for this gene is ligA [H]

Identifier: 161598439

GI number: 161598439

Start: 4417091

End: 4419223

Strand: Reverse

Name: ligA [H]

Synonym: nfa42650

Alternate gene names: 161598439

Gene position: 4419223-4417091 (Counterclockwise)

Preceding gene: 54026237

Following gene: 54026234

Centisome position: 73.39

GC content: 70.56

Gene sequence:

>2133_bases
GTGAGCGACAGCGCGGCTATCTCCGCGGCCGAGGATTCCGCGGCCGCAGCAGCACAGGATCCCGCTTCCGACGAGGATCG
GGTGCGGTGGCAGCGGCTCGCCGAGGAGGTGCGCGAGCATCAGTTCCGCTATTACGTCCGTGATGCCCCGATCATCTCCG
ACGGCGAGTTCGATGCCCTGCTGCGCGAGTTGCAGGAGCTGGAGGACCGGCATCCGGATCTGCGGACGCCGGATTCGCCG
ACGCAGCTGGTGGGCGGCGGGTTCGCCACCGATTTCACCCCGGTGGATCACCTCGAGCGGATGCTGTCGCTGGACAACGT
GTTCGATGTCGACGAGTTGCGCGCCTGGGCGGCGCGGGTGGAGGCCGAGACCGGCCCGGACCTGCACTACCTGTGTGAGG
TGAAGATCGACGGCGTGGCGTTGAACCTGGTGTACGAGCGGGGGAAGTTGGTGCGCGCGGCGACCCGCGGTGACGGCCGC
ACCGGTGAGGACGTCACGCTGAACGCCCGCACGATCGAGGATGTGCCCGGCGAGTTGTCCGCGGGGGGCGAGTTCCCGGT
GCCGGAGCTGCTGGAGGTGCGCGGCGAGGTGTATCTGCGGCTCGCGGATTTCGAGGCGCTGAACGCCGCGATCGTGGCGG
AGGGCAAACCGCCGTACGCCAATCCGCGCAACACGGCGGCGGGGTCGTTGCGGCAGAAGGATCCCGCGGTGACGGCGCGG
CGCCGGTTGCGGATGATCTGTCACGGGCTGGGCCGGATCGAGGGCTACACCCCGGCGTCGCAGTTCGAGGCGTATCGGGC
GCTGTCGGCGTGGGGGCTGCCGGTTTCCGAGCACACCCGCCGGGTGCGGGGCATCGACGCGGTGATCGAGCGCGTGGCCT
ATTGGGGCGAGCACCGCCACGACATCGAGCACGAGATCGACGGCCAGGTGATCAAGGTGGACGAGACGTCGCTGCAGCGG
CGGCTCGGCGCGACCTCCCGGGCGCCGCGGTGGGCGATCGCCTACAAGTATCCGCCGGAGGAGGCGACGACCAAGCTGCG
CGCCATCGAGGTCAACGTCGGCCGCACCGGCCGGGTGACGCCGTTCGCGGTGATGGAGCCGGTGGTGATCGCCGGGTCGA
CGGTGGCGATGGCGACGCTGCACAACGCGTCGGAGGTCAAGCGCAAGGGCGTGTTGATCGGCGACACGGTGACCATCCGC
AAGGCCGGTGACGTGATTCCGGAGGTGCTCGGTCCGGTGGTGGACGCGCGGACCGGCGCCGAACGCGAGTTCGTGATGCC
CACGCACTGCCCGGAGTGCGGTACCGAGCTGGCGCCGGAGAAGGAGGGTGACGCCGACATCCGCTGCCCGAACCAGCGGT
CTTGCCCGGCGCAGCTGCGGGAGCGGGTGTATCACGTGGCCGGCCGCAGCGCTTTCGACATCGAGGCACTCGGATACGAG
GCGGCCATCGACCTGTTGCAGTCCGGTGCGATCGGCGACGAGGGCGACCTGTTCGATCTGGACGAGGCCCGCCTGTTGAC
CACCTCGCTGTTCACCAACAAGAACGGGAGTCTGTCGGCGAACGGCAAACGGCTGCTGGAGAATCTGGACGCGGCCAAGG
ACCGGCCGCTGTGGCGGGTGCTGGTCGGGTTGTCGATCCGGCACGTCGGCCCGACCGCGGCGCGGGCGCTGGCCGCGGAG
TTCGGCAGCATGGAGCGGATCGAGCAGGCGAGCGTGGACGAGCTGGCCGCCACCGACGGGGTGGGGCCGACCATCGCGGC
GGCGGTGGCGGAGTGGTTCACCGTCGATTGGCACCGGGCGATCGTGGCGAAGTGGCGCGCGGCCGGGGTGCGGATGGTCG
ACGAGCGCGATGAGTCGATCGAACGCACGCTGGAGGGGCTGTCGATCGTGGTGACGGGCTCGTTGCAGGGGTTCTCCCGC
GACGGCGCGAAGGAGGCGATCCTGGCGCGGGGCGGCAAGGCGGCCGGTTCGGTGTCGAAGAAGACGGCGTTCGTCGTGGT
CGGGGACGCGCCGGGTGCCAAGGCGGCCAAGGCCGAGGAGCTGGGCGTGCCGATCCTGGACGAGGAGGGGTTCGTGCGGT
TGCTCGCGGAGGGGCCCGCGGCGGTCGCCGCCGAGGAACCGGAATCGGGCTAG

Upstream 100 bases:

>100_bases
CCACCTGTGACGCCCGCCGAGGACGGTGAGCCGACCACTTCCGCCACCTCGGCGGCGGGCCGAGACCCCGCCGCGACGAA
GGAGCGGCAAAATAGACACA

Downstream 100 bases:

>100_bases
CGGGGGCTCACCTGGACGGTGGCGCTGTACTGGCCGCCGATCTCGAGCCTGGTGCAGTGCCCGGGGTCGAGTTCGATGTG
CTGCCGGTTGACCCGGCGCG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] 2 [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSDSAAISAAEDSAAAAAQDPASDEDRVRWQRLAEEVREHQFRYYVRDAPIISDGEFDALLRELQELEDRHPDLRTPDSP
TQLVGGGFATDFTPVDHLERMLSLDNVFDVDELRAWAARVEAETGPDLHYLCEVKIDGVALNLVYERGKLVRAATRGDGR
TGEDVTLNARTIEDVPGELSAGGEFPVPELLEVRGEVYLRLADFEALNAAIVAEGKPPYANPRNTAAGSLRQKDPAVTAR
RRLRMICHGLGRIEGYTPASQFEAYRALSAWGLPVSEHTRRVRGIDAVIERVAYWGEHRHDIEHEIDGQVIKVDETSLQR
RLGATSRAPRWAIAYKYPPEEATTKLRAIEVNVGRTGRVTPFAVMEPVVIAGSTVAMATLHNASEVKRKGVLIGDTVTIR
KAGDVIPEVLGPVVDARTGAEREFVMPTHCPECGTELAPEKEGDADIRCPNQRSCPAQLRERVYHVAGRSAFDIEALGYE
AAIDLLQSGAIGDEGDLFDLDEARLLTTSLFTNKNGSLSANGKRLLENLDAAKDRPLWRVLVGLSIRHVGPTAARALAAE
FGSMERIEQASVDELAATDGVGPTIAAAVAEWFTVDWHRAIVAKWRAAGVRMVDERDESIERTLEGLSIVVTGSLQGFSR
DGAKEAILARGGKAAGSVSKKTAFVVVGDAPGAKAAKAEELGVPILDEEGFVRLLAEGPAAVAAEEPESG

Sequences:

>Translated_710_residues
MSDSAAISAAEDSAAAAAQDPASDEDRVRWQRLAEEVREHQFRYYVRDAPIISDGEFDALLRELQELEDRHPDLRTPDSP
TQLVGGGFATDFTPVDHLERMLSLDNVFDVDELRAWAARVEAETGPDLHYLCEVKIDGVALNLVYERGKLVRAATRGDGR
TGEDVTLNARTIEDVPGELSAGGEFPVPELLEVRGEVYLRLADFEALNAAIVAEGKPPYANPRNTAAGSLRQKDPAVTAR
RRLRMICHGLGRIEGYTPASQFEAYRALSAWGLPVSEHTRRVRGIDAVIERVAYWGEHRHDIEHEIDGQVIKVDETSLQR
RLGATSRAPRWAIAYKYPPEEATTKLRAIEVNVGRTGRVTPFAVMEPVVIAGSTVAMATLHNASEVKRKGVLIGDTVTIR
KAGDVIPEVLGPVVDARTGAEREFVMPTHCPECGTELAPEKEGDADIRCPNQRSCPAQLRERVYHVAGRSAFDIEALGYE
AAIDLLQSGAIGDEGDLFDLDEARLLTTSLFTNKNGSLSANGKRLLENLDAAKDRPLWRVLVGLSIRHVGPTAARALAAE
FGSMERIEQASVDELAATDGVGPTIAAAVAEWFTVDWHRAIVAKWRAAGVRMVDERDESIERTLEGLSIVVTGSLQGFSR
DGAKEAILARGGKAAGSVSKKTAFVVVGDAPGAKAAKAEELGVPILDEEGFVRLLAEGPAAVAAEEPESG
>Mature_709_residues
SDSAAISAAEDSAAAAAQDPASDEDRVRWQRLAEEVREHQFRYYVRDAPIISDGEFDALLRELQELEDRHPDLRTPDSPT
QLVGGGFATDFTPVDHLERMLSLDNVFDVDELRAWAARVEAETGPDLHYLCEVKIDGVALNLVYERGKLVRAATRGDGRT
GEDVTLNARTIEDVPGELSAGGEFPVPELLEVRGEVYLRLADFEALNAAIVAEGKPPYANPRNTAAGSLRQKDPAVTARR
RLRMICHGLGRIEGYTPASQFEAYRALSAWGLPVSEHTRRVRGIDAVIERVAYWGEHRHDIEHEIDGQVIKVDETSLQRR
LGATSRAPRWAIAYKYPPEEATTKLRAIEVNVGRTGRVTPFAVMEPVVIAGSTVAMATLHNASEVKRKGVLIGDTVTIRK
AGDVIPEVLGPVVDARTGAEREFVMPTHCPECGTELAPEKEGDADIRCPNQRSCPAQLRERVYHVAGRSAFDIEALGYEA
AIDLLQSGAIGDEGDLFDLDEARLLTTSLFTNKNGSLSANGKRLLENLDAAKDRPLWRVLVGLSIRHVGPTAARALAAEF
GSMERIEQASVDELAATDGVGPTIAAAVAEWFTVDWHRAIVAKWRAAGVRMVDERDESIERTLEGLSIVVTGSLQGFSRD
GAKEAILARGGKAAGSVSKKTAFVVVGDAPGAKAAKAEELGVPILDEEGFVRLLAEGPAAVAAEEPESG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=87, Percent_Identity=43.6781609195402, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788750, Length=677, Percent_Identity=43.4268833087149, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI87082305, Length=544, Percent_Identity=22.4264705882353, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 76925; Mature: 76794

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSAAISAAEDSAAAAAQDPASDEDRVRWQRLAEEVREHQFRYYVRDAPIISDGEFDAL
CCCCCCEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCHHHH
LRELQELEDRHPDLRTPDSPTQLVGGGFATDFTPVDHLERMLSLDNVFDVDELRAWAARV
HHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
EAETGPDLHYLCEVKIDGVALNLVYERGKLVRAATRGDGRTGEDVTLNARTIEDVPGELS
CCCCCCCEEEEEEEEECCEEEEEEECCCCEEEEECCCCCCCCCCEEEECEECCCCCCCCC
AGGEFPVPELLEVRGEVYLRLADFEALNAAIVAEGKPPYANPRNTAAGSLRQKDPAVTAR
CCCCCCCHHHHHCCCEEEEEEECCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
RRLRMICHGLGRIEGYTPASQFEAYRALSAWGLPVSEHTRRVRGIDAVIERVAYWGEHRH
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHC
DIEHEIDGQVIKVDETSLQRRLGATSRAPRWAIAYKYPPEEATTKLRAIEVNVGRTGRVT
CCCCCCCCCEEEECHHHHHHHHCCCCCCCCEEEEEECCCHHHHCEEEEEEEECCCCCCCC
PFAVMEPVVIAGSTVAMATLHNASEVKRKGVLIGDTVTIRKAGDVIPEVLGPVVDARTGA
CHHHHCCCCCCCCCEEEHHHHCHHHHHHCCEEECCEEEEECHHHHHHHHHHHHHHCCCCC
EREFVMPTHCPECGTELAPEKEGDADIRCPNQRSCPAQLRERVYHVAGRSAFDIEALGYE
CCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
AAIDLLQSGAIGDEGDLFDLDEARLLTTSLFTNKNGSLSANGKRLLENLDAAKDRPLWRV
HHHHHHHCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
LVGLSIRHVGPTAARALAAEFGSMERIEQASVDELAATDGVGPTIAAAVAEWFTVDWHRA
HHCCHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IVAKWRAAGVRMVDERDESIERTLEGLSIVVTGSLQGFSRDGAKEAILARGGKAAGSVSK
HHHHHHHCCCEEEHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCC
KTAFVVVGDAPGAKAAKAEELGVPILDEEGFVRLLAEGPAAVAAEEPESG
CEEEEEEECCCCCCCCCHHHCCCEEECCCCCEEEEECCCCEEECCCCCCC
>Mature Secondary Structure 
SDSAAISAAEDSAAAAAQDPASDEDRVRWQRLAEEVREHQFRYYVRDAPIISDGEFDAL
CCCCCEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCHHHH
LRELQELEDRHPDLRTPDSPTQLVGGGFATDFTPVDHLERMLSLDNVFDVDELRAWAARV
HHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
EAETGPDLHYLCEVKIDGVALNLVYERGKLVRAATRGDGRTGEDVTLNARTIEDVPGELS
CCCCCCCEEEEEEEEECCEEEEEEECCCCEEEEECCCCCCCCCCEEEECEECCCCCCCCC
AGGEFPVPELLEVRGEVYLRLADFEALNAAIVAEGKPPYANPRNTAAGSLRQKDPAVTAR
CCCCCCCHHHHHCCCEEEEEEECCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
RRLRMICHGLGRIEGYTPASQFEAYRALSAWGLPVSEHTRRVRGIDAVIERVAYWGEHRH
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHC
DIEHEIDGQVIKVDETSLQRRLGATSRAPRWAIAYKYPPEEATTKLRAIEVNVGRTGRVT
CCCCCCCCCEEEECHHHHHHHHCCCCCCCCEEEEEECCCHHHHCEEEEEEEECCCCCCCC
PFAVMEPVVIAGSTVAMATLHNASEVKRKGVLIGDTVTIRKAGDVIPEVLGPVVDARTGA
CHHHHCCCCCCCCCEEEHHHHCHHHHHHCCEEECCEEEEECHHHHHHHHHHHHHHCCCCC
EREFVMPTHCPECGTELAPEKEGDADIRCPNQRSCPAQLRERVYHVAGRSAFDIEALGYE
CCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
AAIDLLQSGAIGDEGDLFDLDEARLLTTSLFTNKNGSLSANGKRLLENLDAAKDRPLWRV
HHHHHHHCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
LVGLSIRHVGPTAARALAAEFGSMERIEQASVDELAATDGVGPTIAAAVAEWFTVDWHRA
HHCCHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IVAKWRAAGVRMVDERDESIERTLEGLSIVVTGSLQGFSRDGAKEAILARGGKAAGSVSK
HHHHHHHCCCEEEHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCC
KTAFVVVGDAPGAKAAKAEELGVPILDEEGFVRLLAEGPAAVAAEEPESG
CEEEEEEECCCCCCCCCHHHCCCEEECCCCCEEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA