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The map label for this gene is tuf

Identifier: 161579044

GI number: 161579044

Start: 712351

End: 713535

Strand: Reverse

Name: tuf

Synonym: TC0596

Alternate gene names: 161579044

Gene position: 713535-712351 (Counterclockwise)

Preceding gene: 15835214

Following gene: 15835212

Centisome position: 66.5

GC content: 42.74

Gene sequence:

>1185_bases
ATGTCAAAAGAAACTTTTCAACGTAATAAGCCTCATATCAACATAGGGACCATTGGACACGTTGACCATGGTAAGACTAC
GTTGACAGCTGCTATTACGCGTGCGTTGTCTGGAGATGGGTTGGCTGATTTTCGTGATTATAGCTCTATTGACAACACTC
CTGAAGAAAAAGCTCGCGGTATTACAATCAACGCTTCTCACGTTGAGTACGAGACTCCTAATCGTCACTATGCTCACGTG
GACTGCCCTGGTCACGCCGACTATGTTAAAAACATGATTACTGGTGCGGCTCAGATGGACGGAGCTATTTTAGTAGTTTC
CGCAACAGATGGAGCTATGCCTCAAACTAAAGAGCACATTCTTTTGGCAAGACAGGTTGGGGTTCCTTACATCGTCGTTT
TCCTCAATAAAATTGACATGATTTCCGAAGAAGATGCCGAGCTTGTTGACTTGGTTGAAATGGAGTTGTCTGAGCTTCTT
GAAGAAAAAGGGTATAAAGGTTGCCCAATTATCAGAGGTTCTGCCCTGAAAGCTTTGGAAGGGGATGCTGCTTACATAGA
GAAAGTTCGAGAGCTGATGCAAGCTGTCGATGACAATATCCCTACTCCAGAAAGAGAAATTGATAAACCATTCTTGATGC
CTATTGAGGACGTCTTCTCTATTTCTGGACGAGGAACTGTAGTAACTGGACGTATTGAGCGTGGAATCGTTAAAGTTTCC
GATAAAGTTCAGTTGGTTGGTCTTAGAGATACTAAAGAAACGATCGTTACTGGAGTTGAGATGTTCAGAAAAGAGCTTCC
AGAAGGTCGTGCTGGAGAGAACGTTGGATTGCTCCTCAGAGGTATCGGTAAGAATGATGTAGAACGAGGAATGGTTGTTT
GTTTGCCAAATAGCGTTAAACCTCATACACAATTTAAGTGTGCTGTTTACGTTTTACAAAAAGAAGAAYGTGGTCGTCAT
AAACCATTCTTTACAGGATATAGACCTCAATTCTTCTTCCGTACAACAGATGTTACCGGTGTTGTAACCTTACCTGAAGG
GGTTGAAATGGTCATGCCTGGTGATAACGTTGAATTTGAAGTGCAATTGATCAGCCCTGTAGCTTTGGAAGAGGGAATGA
GATTTGCTATTCGTGAAGGCGGCCGTACAATCGGTGCTGGAACTATTTCTAAGATCATTGCATAA

Upstream 100 bases:

>100_bases
GTAGAGCACTTGCATGGTAAGCAAGTGGCCGTAGGTTCAATTCCTATTCTGGGCAAAAGATATTGGTAGCAAACTAATTT
TTAATTTGAGGATTTTTGAG

Downstream 100 bases:

>100_bases
TTTAAGCGATGTGTTGGCAAGGCTTTCTTGGCATTATTGTAGCCTTGCCTTTTGGGTGTGTAGCTTAGATGGTAGAGCAG
TGGCCTCCAAAGCCGCCGGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLADFRDYSSIDNTPEEKARGITINASHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKIDMISEEDAELVDLVEMELSELL
EEKGYKGCPIIRGSALKALEGDAAYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
DKVQLVGLRDTKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCLPNSVKPHTQFKCAVYVLQKEEXGRH
KPFFTGYRPQFFFRTTDVTGVVTLPEGVEMVMPGDNVEFEVQLISPVALEEGMRFAIREGGRTIGAGTISKIIA

Sequences:

>Translated_394_residues
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLADFRDYSSIDNTPEEKARGITINASHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKIDMISEEDAELVDLVEMELSELL
EEKGYKGCPIIRGSALKALEGDAAYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
DKVQLVGLRDTKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCLPNSVKPHTQFKCAVYVLQKEEXGRH
KPFFTGYRPQFFFRTTDVTGVVTLPEGVEMVMPGDNVEFEVQLISPVALEEGMRFAIREGGRTIGAGTISKIIA
>Mature_393_residues
SKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLADFRDYSSIDNTPEEKARGITINASHVEYETPNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKIDMISEEDAELVDLVEMELSELLE
EKGYKGCPIIRGSALKALEGDAAYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVSD
KVQLVGLRDTKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCLPNSVKPHTQFKCAVYVLQKEEXGRHK
PFFTGYRPQFFFRTTDVTGVVTLPEGVEMVMPGDNVEFEVQLISPVALEEGMRFAIREGGRTIGAGTISKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=54.911838790932, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=30.045871559633, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=30.045871559633, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=29.0465631929047, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=28.0623608017817, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=29.2452830188679, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI194097354, Length=324, Percent_Identity=28.7037037037037, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194018520, Length=324, Percent_Identity=28.7037037037037, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI194018522, Length=324, Percent_Identity=28.7037037037037, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=29.0123456790123, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI82546879, Length=427, Percent_Identity=25.0585480093677, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4503507, Length=299, Percent_Identity=28.4280936454849, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI94966754, Length=146, Percent_Identity=35.6164383561644, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI157426893, Length=303, Percent_Identity=25.7425742574257, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=66.9191919191919, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=66.9191919191919, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI2367247, Length=369, Percent_Identity=30.8943089430894, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=35.4166666666667, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789108, Length=344, Percent_Identity=26.7441860465116, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788922, Length=269, Percent_Identity=27.5092936802974, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=51.4925373134328, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.5876288659794, Blast_Score=330, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=332, Percent_Identity=32.8313253012048, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=332, Percent_Identity=32.8313253012048, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=302, Percent_Identity=33.4437086092715, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=27.2108843537415, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=445, Percent_Identity=26.9662921348315, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=436, Percent_Identity=25.6880733944954, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=27.8388278388278, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=269, Percent_Identity=30.1115241635688, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17565438, Length=430, Percent_Identity=25.1162790697674, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=290, Percent_Identity=26.551724137931, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=62.2166246851385, Blast_Score=498, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6325337, Length=348, Percent_Identity=31.8965517241379, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=348, Percent_Identity=31.8965517241379, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=465, Percent_Identity=24.5161290322581, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=353, Percent_Identity=27.1954674220963, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=294, Percent_Identity=28.2312925170068, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=31.7991631799163, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=152, Percent_Identity=30.9210526315789, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=56.4556962025316, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=56.4556962025316, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=48.4536082474227, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=330, Percent_Identity=31.5151515151515, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI45553816, Length=330, Percent_Identity=31.5151515151515, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24651721, Length=330, Percent_Identity=31.5151515151515, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17864154, Length=330, Percent_Identity=31.5151515151515, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=28.0269058295964, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=28.0269058295964, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=31.0650887573964, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922690, Length=301, Percent_Identity=31.2292358803987, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=29.3948126801153, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=28.3154121863799, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=28.3154121863799, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI78706572, Length=274, Percent_Identity=28.4671532846715, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28572034, Length=138, Percent_Identity=39.1304347826087, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43293; Mature: 43162

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLADFRDYSSIDNTPEEKARG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCC
ITINASHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKIDMISEEDAELVDLVEMELSELLEEKGYKGCPIIRGSALKALE
EEEHHCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCC
GDAAYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
CCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCCEEEEC
DKVQLVGLRDTKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCLPNSVK
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCC
PHTQFKCAVYVLQKEEXGRHKPFFTGYRPQFFFRTTDVTGVVTLPEGVEMVMPGDNVEFE
CCCCEEEEEEEEEECCCCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEE
VQLISPVALEEGMRFAIREGGRTIGAGTISKIIA
EEEECCHHHHHCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
SKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSGDGLADFRDYSSIDNTPEEKARG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCC
ITINASHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKIDMISEEDAELVDLVEMELSELLEEKGYKGCPIIRGSALKALE
EEEHHCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCC
GDAAYIEKVRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGIVKVS
CCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCCEEEEC
DKVQLVGLRDTKETIVTGVEMFRKELPEGRAGENVGLLLRGIGKNDVERGMVVCLPNSVK
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCC
PHTQFKCAVYVLQKEEXGRHKPFFTGYRPQFFFRTTDVTGVVTLPEGVEMVMPGDNVEFE
CCCCEEEEEEEEEECCCCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEE
VQLISPVALEEGMRFAIREGGRTIGAGTISKIIA
EEEECCHHHHHCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA