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The map label for this gene is trmE [H]

Identifier: 161526321

GI number: 161526321

Start: 3443231

End: 3444625

Strand: Reverse

Name: trmE [H]

Synonym: Bmul_3152

Alternate gene names: 161526321

Gene position: 3444625-3443231 (Counterclockwise)

Preceding gene: 161526323

Following gene: 161526318

Centisome position: 99.89

GC content: 69.75

Gene sequence:

>1395_bases
ATGCTCGCTACCGATTCCGATCCGATCGTCGCCATTGCCACCGCTGCCGGCCGGGGCGGCATCGGCGTCGTCCGCGTATC
GTTCGGGCGCGGCGGCGAGGCGGCGGCGCTGCCGATGATCGACGCGCTATGCGGCCAGCGGCTCGCGCCGCGTCATGCGA
GCTACGTGCCGTTCCTCGACGCGCAGGGCGCGCCGCTCGATCGCGGCATCGCGCTGTACTTCCCCGCGCCGCACTCATAT
ACGGGCGAGCACGTGCTCGAATTGCAGGGGCACGGCGGCCCGATCGTGATGCAGCTGCTGCTGCAGCGCTGCCTCGACGT
CGGTCGCGCGGTCGGGCTGCGGCTCGCGGAGCCCGGCGAATTCACGCGCCGCGCATTCCTGAACGACAAGCTCGATCTCG
CGCAGGCCGAAGCGGTCGCCGATCTGATCGAGGCGAGCACCGAGGCCGCCGCGCGCTCGGCCGGCCGCTCGCTCGACGGG
GCGTTCTCGCGGCAGATTCATGCGCTCGTCGACGACGTGATCAATCTGCGGATGCTGGTCGAGGCGACGCTCGATTTTCC
GGAGGAGGAAATCGACTTTCTCGAAGCGGCCGACGCGCGCGGCAAGCTCGCGAAGATCCGTGCGCAGCTCGCGCACGTGC
TCGGCGATGCGCGGCAGGGCGCGCTGCTGCGCGAAGGGCTGTCGGTCGTGCTCGCGGGGCAGCCGAACGTCGGCAAGTCG
TCGCTGCTGAACGCGCTGGCCGGCGCGGAGCTCGCGATCGTCACGCCGATCGCCGGCACGACGCGCGACAAGGTCGCGCA
GACGATCCAGGTCGAAGGCATTCCGCTGCACATCATCGATACGGCGGGCCTGCGCGAGACCGAGGACGAAGTCGAGCGGA
TCGGGATCGCGCGCACGTGGAGCGAGATCGAGCGCGCGGACGTCGTGCTGCATCTGCTCGATTCGCGGACCGGAATGACC
GCGGACGACGACGCGATCGCCGCGCGTTTCCCGGCCGGCGTGCCGGTCGTGCGCGTGCTGAACAAGACGGATCTGACCGG
CGTGCCGGCATCGGTCGCGCATCCGGCCGCGGAAGGCGATCTGACGGAAGTGCATCTGTCCGCGAAGCGCGGCGACGGGA
TCGACCTGCTGCGTGCGGAGCTGCTGCGCATCGCCGGCTGGCAGGCCGGCGCGGAAGGCGTCTATCTCGCGCGCGAGCGG
CATCTGATGGCGCTGCGCGCCGCGCAGGATCACCTCGCGCGCGCGGCCGAGCATGCGGAACAGCGTGCGCAGTCGCTCGA
CCTGTTCGCGGAGGAGCTTCGGCTCGCGCAGGAGCAGCTCAATGCGATTACGGGGGAATTTACTTCGGACGATCTGCTGG
GCGTGATCTTCAGCAGGTTCTGTATCGGGAAGTGA

Upstream 100 bases:

>100_bases
CCGGCCGTGCAGGTGCGGGCGCGCTACAATGCCGGCGTTCGTTTGCCCGATCTGTTGCGCGGGCGCACCACTGACTGCCT
GATTACCGCCTCCGATCCCC

Downstream 100 bases:

>100_bases
CGCGGCAATCGCCGCCGTCACTTCACCTTCAACGCCGCGCGCCGAGGCGCGGCAAGCGGAGCGTCATCGCGTTGCCGAAG
CGTCCGCGTGCGCAGTATCG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPMIDALCGQRLAPRHASYVPFLDAQGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDVGRAVGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVINLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRTGMT
ADDDAIAARFPAGVPVVRVLNKTDLTGVPASVAHPAAEGDLTEVHLSAKRGDGIDLLRAELLRIAGWQAGAEGVYLARER
HLMALRAAQDHLARAAEHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK

Sequences:

>Translated_464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPMIDALCGQRLAPRHASYVPFLDAQGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDVGRAVGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVINLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRTGMT
ADDDAIAARFPAGVPVVRVLNKTDLTGVPASVAHPAAEGDLTEVHLSAKRGDGIDLLRAELLRIAGWQAGAEGVYLARER
HLMALRAAQDHLARAAEHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
>Mature_464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPMIDALCGQRLAPRHASYVPFLDAQGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDVGRAVGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVINLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRTGMT
ADDDAIAARFPAGVPVVRVLNKTDLTGVPASVAHPAAEGDLTEVHLSAKRGDGIDLLRAELLRIAGWQAGAEGVYLARER
HLMALRAAQDHLARAAEHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=476, Percent_Identity=37.6050420168067, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=37.1244635193133, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI193082988, Length=506, Percent_Identity=34.9802371541502, Blast_Score=218, Evalue=7e-57,
Organism=Escherichia coli, GI2367268, Length=470, Percent_Identity=54.2553191489362, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI87082120, Length=117, Percent_Identity=41.8803418803419, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=467, Percent_Identity=29.9785867237687, Blast_Score=161, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=501, Percent_Identity=28.9421157684631, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI45550813, Length=491, Percent_Identity=32.5865580448065, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49376; Mature: 49376

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPMIDALCGQRLAPRHASYVPFLD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEC
AQGAPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVMQLLLQRCLDVGRAVGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRQIHALVDDVINLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
SEIERADVVLHLLDSRTGMTADDDAIAARFPAGVPVVRVLNKTDLTGVPASVAHPAAEGD
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCC
LTEVHLSAKRGDGIDLLRAELLRIAGWQAGAEGVYLARERHLMALRAAQDHLARAAEHAE
CEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
QRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPMIDALCGQRLAPRHASYVPFLD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEC
AQGAPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVMQLLLQRCLDVGRAVGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRQIHALVDDVINLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
SEIERADVVLHLLDSRTGMTADDDAIAARFPAGVPVVRVLNKTDLTGVPASVAHPAAEGD
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCC
LTEVHLSAKRGDGIDLLRAELLRIAGWQAGAEGVYLARERHLMALRAAQDHLARAAEHAE
CEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
QRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA