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The map label for this gene is topB [H]

Identifier: 161526296

GI number: 161526296

Start: 3414291

End: 3416888

Strand: Direct

Name: topB [H]

Synonym: Bmul_3127

Alternate gene names: 161526296

Gene position: 3414291-3416888 (Clockwise)

Preceding gene: 161526295

Following gene: 161526298

Centisome position: 99.01

GC content: 63.93

Gene sequence:

>2598_bases
ATGTCCAAAGCACTGATCATTGCGGAAAAGCCTTCTGTCGCGAACGACATCGCGCGCGCTTTGGGCGGCTTTACCAAGCA
TGACGAATACTTCGAAAGCGACGAATACGTGCTTTCGTCCGCTGTCGGCCACCTGCTGGAAATCGCCGCTCCGGAAGAGT
ACGAGGTCAAGCGCGGGAAATGGAGCTTCGCGCATCTGCCCGTCATCCCCCCGCATTTCGACCTCAACCCGATCGCAAAA
AGCGAGTCGCGCCTGAAAGTGCTGACGAAGCTGATGAAGCGCAAGGACGTCGATCGCCTGATCAACGCATGCGACGCGGG
GCGCGAGGGCGAGCTGATTTTCCGCCTGATCGTGCAGCACGCGAAGGCGAAGCAGCCGGTCCAGCGCCTGTGGCTGCAAT
CGATGACGCCGCAGGCGATCCGCGACGGTTTCGCGCACCTGCGCAGCGACGCCGACATGCAGCCGCTCGCCGACGCCGCG
CGCTGCCGTTCCGAAGCCGACTGGCTCGTCGGCATCAACGGCACGCGGGCGATGACGGCCTTCAACAGCAAGGGCGGCGG
CTTTTTCCTGACGACCGTCGGGCGCGTGCAGACGCCGACCTTGTCGATCGTCGTCGAACGCGAAGAGAAAATCCGCCGTT
TCGTCCCGCGCGACTACTGGGAAGTGAAGGCGGAATTCGCATGCGCGGCCGGCTTCTACGAAGGCAAGTGGTTCGACCCG
AAATTCAAGCGCGACGAATTCGATCCGGAGAAGCGCGATTCGCGGCTCTGGAGCCTGCCGGCTGCCGAAACGATCGTCGC
TGCGTGTCGCGACCAGGTCGGCACGGTCAGCGAGGAATCGAAGCCGTCGACGCAGCTGTCGCCGCTGCTGTTCGATCTGA
CGAGCCTGCAGCGCGAGGCGAACAGCCGTTTCGGCTTCTCCGCGAAGAACACGCTGGGCCTCGCGCAGGCGCTGTATGAA
AAGCACAAGGTGCTGACCTACCCGCGTACCGATGCGCGCGCGCTGCCGGAGGACTATATCGGCACGGTGAAGTCGACGCT
CGAGATGCTCAAGGAGAGCCACAACTACCTGCCGCACGCGAAGCAGGTGCTCGACAAGGGCTGGGTCAAGCCGAACAAGC
GGATCTTCGACAACTCGAAGATCAGCGATCACTTTGCGATCATCCCGACGCTGCAGGCGCCGAAATCGCTGTCCGAGCCC
GAGCAGAAGCTCTACGACATGGTCGTGAAGCGCTTCCTCGCCGTGTTCTTCCCGGCCGCCGAATACCGCGTGACGACGCG
CATCACCGAAGTCGCCGGCCATCACTTCAAGACCGAAGGCAAGGTGCTCGTCGAGCCGGGCTGGCTGCAGGTCTACGGCC
GCGACGCCGAAGGCGCCGACGCGAACCTCGTGCAGGTGCAGAAGGACGAGAAGGTGAAGACGGACGAAATCGCCGCCGTC
GCGCTCGTCACGAAGCCGCCCGCACGCTACTCGGAAGCGACGCTGCTGTCGGCGATGGAAGGCGCGGGCAAGCTCGTCGA
GGACGACGAACTGCGCGAGGCGATGGCCGCGAAGGGCCTCGGTACGCCGGCCACGCGCGCGGCGATCATCGAAGGCCTGC
TCGGCGAGAAATATCTCGTGCGCGAAGGCCGCGAACTGATCCCGACCGCGAAGGCATTCCAGCTGATGACGCTGCTGCGC
GGGCTCGGCGTGAAGGAACTGACCGCACCCGAGCTCACGGGCGAATGGGAATACAAGCTGTCGCAGATGGAGCGCGGCAA
CCTCGGACGCGACGCATTCATGCAGGAAATCGCGCGCATGACGCAGCAGATCGTCAAGCGCGCGAAGGAATACGATTCCG
ACACGATTCCCGGCGATTACGCGACGCTCGAAACGCCGTGCCCGAACTGCGGCGGTCAGGTGAAGGAGAACTACCGCCGC
TTCGCGTGCACGAAGTGCGACTTCTCGATCTCGAAGATTCCGGGCAGCCGGCAGTTCGAGATCGCCGAAGTCGAGGAGCT
GTTGCAGAAGAAGGAGATCGGTCCGCTGTCGGGCTTCCGCAGCAAGATGGGCCGTCCGTTCTCGGCGATCCTGAAGCTGA
CGTTCGACGACGAAATCAAGAACTACAAGCTCGAGTTCGATTTCGGGCAGGATCAGGGCGGCGACGAAGGCGAAGCGCCC
GACTTCTCGGCGCAGGAGCCGGTCGGCGCGTGCCCGAAGTGCAAGGGTCGCGTGTTCGAGCACGGGATGAGCTACGTCTG
CGAGCACTCGGTCGCGAACCCGAAGACGTGCGACTTCCGCTCCGGCAAGGTGATCCTGCAGCAGGAGATCACGCGCGAGC
AGATGGCCAAGCTGCTCGCGGACGGCCGCACCGATCTGCTGCCGAACTTCAAGTCGTCGCGCACGGGCCGCAACTTCAAG
GCGTATCTCGTGAAGCAGCCGGACGGCAAGATCGGCTTCGAGTTCGAGAAGAAGGAGCCGAAGGCGGCCGCCGCGAAGAA
GACGGCGAAATCGGCGACGAAGGACACGGAGACCGTCACCGAAGGCGCCGACGAGAAGCCGGCGCCGGCACGCAAGACCG
CCGCACGCAAGACGACGACGCGCAAGACGGGCTCGTAA

Upstream 100 bases:

>100_bases
GCTTTTTACACGTCCGGCCGCCGTCGCGGCGGCGTGTGCTATAAAGCCGTCGTAAAAGGGACCCACACCGGCGAACCCGG
TCTACCAACACACATCTGTC

Downstream 100 bases:

>100_bases
CGAGCGGCGCCGCGAGCCGCGCGCACGTCGCGGCGGCTCGCCGGCCCGCCGGCTTCGTCGGGCAAACAAAAAACGCGGAT
CGATCGCTCGATCCGCGTTT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 865; Mature: 864

Protein sequence:

>865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYFESDEYVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQHAKAKQPVQRLWLQSMTPQAIRDGFAHLRSDADMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFACAAGFYEGKWFDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYIGTVKSTLEMLKESHNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEP
EQKLYDMVVKRFLAVFFPAAEYRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVQVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACTKCDFSISKIPGSRQFEIAEVEELLQKKEIGPLSGFRSKMGRPFSAILKLTFDDEIKNYKLEFDFGQDQGGDEGEAP
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMAKLLADGRTDLLPNFKSSRTGRNFK
AYLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDTETVTEGADEKPAPARKTAARKTTTRKTGS

Sequences:

>Translated_865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYFESDEYVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQHAKAKQPVQRLWLQSMTPQAIRDGFAHLRSDADMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFACAAGFYEGKWFDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYIGTVKSTLEMLKESHNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEP
EQKLYDMVVKRFLAVFFPAAEYRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVQVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACTKCDFSISKIPGSRQFEIAEVEELLQKKEIGPLSGFRSKMGRPFSAILKLTFDDEIKNYKLEFDFGQDQGGDEGEAP
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMAKLLADGRTDLLPNFKSSRTGRNFK
AYLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDTETVTEGADEKPAPARKTAARKTTTRKTGS
>Mature_864_residues
SKALIIAEKPSVANDIARALGGFTKHDEYFESDEYVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAKS
ESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQHAKAKQPVQRLWLQSMTPQAIRDGFAHLRSDADMQPLADAAR
CRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFACAAGFYEGKWFDPK
FKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYEK
HKVLTYPRTDARALPEDYIGTVKSTLEMLKESHNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPE
QKLYDMVVKRFLAVFFPAAEYRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVQVQKDEKVKTDEIAAVA
LVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLRG
LGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRF
ACTKCDFSISKIPGSRQFEIAEVEELLQKKEIGPLSGFRSKMGRPFSAILKLTFDDEIKNYKLEFDFGQDQGGDEGEAPD
FSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMAKLLADGRTDLLPNFKSSRTGRNFKA
YLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDTETVTEGADEKPAPARKTAARKTTTRKTGS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=649, Percent_Identity=26.040061633282, Blast_Score=144, Evalue=4e-34,
Organism=Homo sapiens, GI4507635, Length=700, Percent_Identity=23.7142857142857, Blast_Score=122, Evalue=2e-27,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=34.0872374798061, Blast_Score=331, Evalue=1e-91,
Organism=Escherichia coli, GI1787529, Length=669, Percent_Identity=26.1584454409567, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=626, Percent_Identity=25.3993610223642, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=633, Percent_Identity=23.696682464455, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585251, Length=651, Percent_Identity=25.4992319508449, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640096, Length=640, Percent_Identity=22.1875, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96608; Mature: 96477

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALIIAEKPSVANDIARALGGFTKHDEYFESDEYVLSSAVGHLLEIAAPEEYEVKRGK
CCCEEEEECCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFAHLRSDADMQPLADAARCRSEADWLVGINGTRAMTA
HHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFACAAGFYEGKWFDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYIGTVKSTLEMLKESHNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPEQKLYDMVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EYRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVQVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEEECCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLV
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACTKCDFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCEE
IAEVEELLQKKEIGPLSGFRSKMGRPFSAILKLTFDDEIKNYKLEFDFGQDQGGDEGEAP
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMAKLLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLLPNFKSSRTGRNFKAYLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDTETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHCCCHHHHH
EGADEKPAPARKTAARKTTTRKTGS
CCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKALIIAEKPSVANDIARALGGFTKHDEYFESDEYVLSSAVGHLLEIAAPEEYEVKRGK
CCEEEEECCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFAHLRSDADMQPLADAARCRSEADWLVGINGTRAMTA
HHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFACAAGFYEGKWFDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYIGTVKSTLEMLKESHNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPEQKLYDMVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EYRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVQVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEEECCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLV
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACTKCDFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCEE
IAEVEELLQKKEIGPLSGFRSKMGRPFSAILKLTFDDEIKNYKLEFDFGQDQGGDEGEAP
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMAKLLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLLPNFKSSRTGRNFKAYLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDTETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHCCCHHHHH
EGADEKPAPARKTAARKTTTRKTGS
CCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA