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The map label for this gene is rsmB [H]

Identifier: 161526289

GI number: 161526289

Start: 3407338

End: 3408804

Strand: Reverse

Name: rsmB [H]

Synonym: Bmul_3120

Alternate gene names: 161526289

Gene position: 3408804-3407338 (Counterclockwise)

Preceding gene: 161526290

Following gene: 161526288

Centisome position: 98.85

GC content: 71.78

Gene sequence:

>1467_bases
ATGCGCGGTTTGGGATCGGCGCGGCGTCGCGCGGCTCCCGCTCCGCTTGCCGATTTCGCTCCGATGACACGAACCCGTTC
CGCTTCCCCGTCTTCTTCCGGCCGCGCCGCACGCCTGTCGGCATTGCGCGTCGCGCCCGACTCGCTCGGCTTCGCGCTCG
ACGCGGCCGCGCAGGCGGTCGACGCGGTGCGGCGCGGCACCGCGTTGCCGGCCGCGCTCTCCGCGGTGTTCGCTCAGATG
GCATCGGGTGCGCAGGCGCTCGCGCGCGGTGCGACGCAGGACGTCGCGTATCGCACGATGCGCCGTCTCGGCAGCGTCGA
CTGGCTGATCGCGCGCCTCGTCGGCAAGGCGCCGCCCGCACACGTGCATGCGGTGCTCGCATGTGCGCTGGCGCTGCTGC
TCGACGCGGACGGCGAAGCGGCGTATCCGCCGTTCACGGTCGTCGATCAGGCCGTGACCGCGATCGGCGCGCGGCGCGAA
TATGCGTTTGCGAAGGGGATGGTGAACGCCGTGCTGCGACGCTTCCTGCGCGAACGCGACACGCTCGTCGCCGCGATGCA
TGCCGATCCGGTCGCGCAATGGAACTACCGCGCATGGTGGATCGACGCCGTGAAGCGCGCATGGCCCGACGCCTGGCAGG
CGATACTCGCGGCCGGCGACCGGCAGGGACCGCTGACGTTGCGCGTGAACGCGCGTCGCACGAGCGTCGACGCGTATCTC
GGCACGCTGCGCGATAACGGAATCGAGGCCGCTGCGATCGGCCGGCATGCGGTGCGGCTCGCATCGGCGCTGCCGGTCGA
GCGCATTCCGGGTTTCGCCGACGGCGTCGTGTCGGTGCAGGATGCGGGCGCCCAGCTCGCGGCCGAATGGCTCGGCGCGC
GCGACGGCATGCGCGCGCTCGATGCCTGCGCGGCACCGGGCGGCAAGACCGGCCATATCCTTGAACTCGCCGACGCGGAA
GTCGTTGCGCTCGAAAGCGACGCGAGCCGCGCGACGCGCATCGGCGAGAACCTCGCGCGGCTGTCGCTCGCGGCCGACGT
GCGCGTCGGCGATGCGGGCGCGCCCGACGCCTGGTACGACGGCCGTCCGTTCGACCGGATCCTGGCCGACGTGCCGTGCT
CGGCGTCCGGGATCGTGCGACGTCATCCGGACATCCGCTGGCTGCGCCGCGAAGCCGACATCCCGGCGCTCGTCGCCGAA
CAGCGGCGCATCCTCTCGGCGCTGTGGCCGCTCGTGAAGCCGGGCGGCGAACTGTTGTACGTCACGTGCTCGATCTTCCC
CGAAGAAGGCGAGCAGCAGGCGCGCTGGTTTGAAGCGGCCTGCGAAGATGCGGTACGATTGGACGCGCCCGGCCAGCTGC
TGCCGCGTGCCGCTTCGGGCGGAGCGGGCAGCGAAGGCGCCGCCGGCGTGCCCGACCCGACCACCGATCACGACGGATTT
TTCTACGCGCGGTTTCAGAAACGGTGA

Upstream 100 bases:

>100_bases
GGCGCCTGTTCCGCGGTCCTTCCCCGATCACCCCGCATGCGTCGCGCTGCGGGGTGATTCATTTGCGCGTCGTCCGGGCC
CCATCCGGTCGCACGCTACA

Downstream 100 bases:

>100_bases
CGATCAAACACCTTTTTCCACTTCGGCTCGCGGCCGTCCTGCTGGTCGCGTTGACGCTGTGCGTGGCCATCGTGCGGCCG
GCGCACGCCGAATCGATTGC

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MRGLGSARRRAAPAPLADFAPMTRTRSASPSSSGRAARLSALRVAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQM
ASGAQALARGATQDVAYRTMRRLGSVDWLIARLVGKAPPAHVHAVLACALALLLDADGEAAYPPFTVVDQAVTAIGARRE
YAFAKGMVNAVLRRFLRERDTLVAAMHADPVAQWNYRAWWIDAVKRAWPDAWQAILAAGDRQGPLTLRVNARRTSVDAYL
GTLRDNGIEAAAIGRHAVRLASALPVERIPGFADGVVSVQDAGAQLAAEWLGARDGMRALDACAAPGGKTGHILELADAE
VVALESDASRATRIGENLARLSLAADVRVGDAGAPDAWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAE
QRRILSALWPLVKPGGELLYVTCSIFPEEGEQQARWFEAACEDAVRLDAPGQLLPRAASGGAGSEGAAGVPDPTTDHDGF
FYARFQKR

Sequences:

>Translated_488_residues
MRGLGSARRRAAPAPLADFAPMTRTRSASPSSSGRAARLSALRVAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQM
ASGAQALARGATQDVAYRTMRRLGSVDWLIARLVGKAPPAHVHAVLACALALLLDADGEAAYPPFTVVDQAVTAIGARRE
YAFAKGMVNAVLRRFLRERDTLVAAMHADPVAQWNYRAWWIDAVKRAWPDAWQAILAAGDRQGPLTLRVNARRTSVDAYL
GTLRDNGIEAAAIGRHAVRLASALPVERIPGFADGVVSVQDAGAQLAAEWLGARDGMRALDACAAPGGKTGHILELADAE
VVALESDASRATRIGENLARLSLAADVRVGDAGAPDAWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAE
QRRILSALWPLVKPGGELLYVTCSIFPEEGEQQARWFEAACEDAVRLDAPGQLLPRAASGGAGSEGAAGVPDPTTDHDGF
FYARFQKR
>Mature_488_residues
MRGLGSARRRAAPAPLADFAPMTRTRSASPSSSGRAARLSALRVAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQM
ASGAQALARGATQDVAYRTMRRLGSVDWLIARLVGKAPPAHVHAVLACALALLLDADGEAAYPPFTVVDQAVTAIGARRE
YAFAKGMVNAVLRRFLRERDTLVAAMHADPVAQWNYRAWWIDAVKRAWPDAWQAILAAGDRQGPLTLRVNARRTSVDAYL
GTLRDNGIEAAAIGRHAVRLASALPVERIPGFADGVVSVQDAGAQLAAEWLGARDGMRALDACAAPGGKTGHILELADAE
VVALESDASRATRIGENLARLSLAADVRVGDAGAPDAWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAE
QRRILSALWPLVKPGGELLYVTCSIFPEEGEQQARWFEAACEDAVRLDAPGQLLPRAASGGAGSEGAAGVPDPTTDHDGF
FYARFQKR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=325, Percent_Identity=27.0769230769231, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI76150623, Length=325, Percent_Identity=27.0769230769231, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI32698918, Length=276, Percent_Identity=30.0724637681159, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI2367212, Length=441, Percent_Identity=37.8684807256236, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI87081985, Length=290, Percent_Identity=31.3793103448276, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17536757, Length=269, Percent_Identity=29.368029739777, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324268, Length=337, Percent_Identity=26.7062314540059, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319447, Length=177, Percent_Identity=32.7683615819209, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI22024126, Length=267, Percent_Identity=27.3408239700375, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24668781, Length=236, Percent_Identity=33.8983050847458, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356513, Length=231, Percent_Identity=33.7662337662338, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355201, Length=216, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 51830; Mature: 51830

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLGSARRRAAPAPLADFAPMTRTRSASPSSSGRAARLSALRVAPDSLGFALDAAAQAV
CCCCCCHHHHCCCCCHHHHCCCHHCCCCCCCCCCCHHHHHHHEECCHHHHHHHHHHHHHH
DAVRRGTALPAALSAVFAQMASGAQALARGATQDVAYRTMRRLGSVDWLIARLVGKAPPA
HHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
HVHAVLACALALLLDADGEAAYPPFTVVDQAVTAIGARREYAFAKGMVNAVLRRFLRERD
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLVAAMHADPVAQWNYRAWWIDAVKRAWPDAWQAILAAGDRQGPLTLRVNARRTSVDAYL
HEEEEECCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
GTLRDNGIEAAAIGRHAVRLASALPVERIPGFADGVVSVQDAGAQLAAEWLGARDGMRAL
HHHHCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHH
DACAAPGGKTGHILELADAEVVALESDASRATRIGENLARLSLAADVRVGDAGAPDAWYD
HHHCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCC
GRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLY
CCCHHHHHHCCCCCCCCCHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
VTCSIFPEEGEQQARWFEAACEDAVRLDAPGQLLPRAASGGAGSEGAAGVPDPTTDHDGF
EEEEECCCCCHHHHHHHHHHHHHHEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCE
FYARFQKR
EEEEEECC
>Mature Secondary Structure
MRGLGSARRRAAPAPLADFAPMTRTRSASPSSSGRAARLSALRVAPDSLGFALDAAAQAV
CCCCCCHHHHCCCCCHHHHCCCHHCCCCCCCCCCCHHHHHHHEECCHHHHHHHHHHHHHH
DAVRRGTALPAALSAVFAQMASGAQALARGATQDVAYRTMRRLGSVDWLIARLVGKAPPA
HHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
HVHAVLACALALLLDADGEAAYPPFTVVDQAVTAIGARREYAFAKGMVNAVLRRFLRERD
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLVAAMHADPVAQWNYRAWWIDAVKRAWPDAWQAILAAGDRQGPLTLRVNARRTSVDAYL
HEEEEECCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
GTLRDNGIEAAAIGRHAVRLASALPVERIPGFADGVVSVQDAGAQLAAEWLGARDGMRAL
HHHHCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHH
DACAAPGGKTGHILELADAEVVALESDASRATRIGENLARLSLAADVRVGDAGAPDAWYD
HHHCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCC
GRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLY
CCCHHHHHHCCCCCCCCCHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
VTCSIFPEEGEQQARWFEAACEDAVRLDAPGQLLPRAASGGAGSEGAAGVPDPTTDHDGF
EEEEECCCCCHHHHHHHHHHHHHHEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCE
FYARFQKR
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA