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The map label for this gene is alkJ [H]

Identifier: 161526236

GI number: 161526236

Start: 3350164

End: 3351804

Strand: Reverse

Name: alkJ [H]

Synonym: Bmul_3067

Alternate gene names: 161526236

Gene position: 3351804-3350164 (Counterclockwise)

Preceding gene: 161526238

Following gene: 161526229

Centisome position: 97.2

GC content: 68.56

Gene sequence:

>1641_bases
ATGCAATACGACTACATCATCGTCGGCGGCGGCTCGGGCGGTTCGAGCGTCGCCGCGCGCCTCGCCGATGCGTGCCCGGA
CGCGACGATCGCACTCATCGAAGCCGGCCCGCACACCGAGCGCAATCTGCTCGTCAACATGCCGGTCGGCATCGCGGCGC
TCGTGCCGTTCAAGCTGCGCACGAACTACGGTTACGAGACCGTGCCGCAGCCGGGCTTGGGCGGGCGTCGCGGCTATCAG
CCGCGCGGCCGCGGCATGGGCGGCTCGAGCGCGATCAACGCGATGATCTATACGCGCGGCCATCCGAGCGACTACGACGA
ATGGGCGCAGCTCGGCGCGACCGGCTGGGGCTGGCAGGACGTGCTGCCGTATTTCCGCCGCGCGGAAGGCAACCAGCGCG
GCGCCGACGCATGGCATGGCGCCGACGGTCCGCTGACGGTATCGGATCTGCGCTTTCGCAATCCGTTCTCCGAACGATTC
ATCGAGGCCGCGCATGCGGCCGGCTATCCGCTCAACGACGACTTCAACGGCGCGACGCAGGAAGGCGTCGGCTTCTATCA
GGTCACGCATCGCGACGGTTCGCGCTGCAGCGTCGCGCGCGCGTACATCTACGGCCGCAACCGGCCGAACCTGCACGTGA
TCACCGATGCGACCGTGCTGCGCGTCGGCTTCGACGGCAAGCGTGCGGTCGGCGTCGCGGTGTCGCGCAACGGGCGCATC
GAGACGCTCGGCGCGCGCGCGGAAGTGATCCTGTCGGCGGGCGCGTTCAACTCGCCGCAACTGCTGATGTGCTCGGGCAT
CGGGCCCGCCGCGCAGTTGCGCCGGCACGGGATCGCGGTCGTGCACGATGCGCCGGACGTCGGCGAAAACCTGATCGACC
ACATCGACTTCATCATCAACACGCGCGTGAATTCGTCGGAGCTGGTCGGCATCTGCCTGCGCGGGATCGCGAAGATGACG
CCCGCGCTCGCGCGCTATTTCTCGAGCCGCACGGGAATGATGACGAGCAACGTCGCGGAAGCGGGCGGCTTCATCAAGAG
CGATCCGTCGCTCGATCGGCCGGACCTGCAGCTGCATTTCTGCACGGCGCTCGTCGACGATCACAATCGCAAGATGCACT
GGGGATTCGGCTATTCGCTGCACGTGTGTGCGCTGCGTCCGTACAGCCGCGGCACGGTCGCACTCGCGAGCGGCGACGCG
CGCGACCCGCCGCTGATCGATCCGCGCTTTTTCAGCGATTCGCGCGATCTCGACCTGCTCGTGCGCGGTGCGCAGGCGAT
GCGGCGCATCCTGTCGCAGCCGCCGCTCGCCTCGCACGGCGGTCGCGAGCTGTACACGCATCCGGATCAGAGCGAAGCCG
AGCTGCGCGAAGCGATCGTCGCGCATGCGGACACGATCTATCACCCGGTCGGCACCTGCCGGATGGGCTCCGACGCGCGT
GCGGTCGTCGATCCGCAATTGCGCGTGCGCGGCGTCGAAGGGTTGCGCGTCGTCGATGCGTCCGTGATGCCGACGCTGAT
CGGCGGCAACACGAATGCGCCGTCGGTGATGATCGGCGAACGCGCGGCGGACTTCATCGTTGCCGCGCGCAAGGGCGGCG
TGCCGCGCGATCAGCCCGCCGCTACCGTGCACGGCCGCTGA

Upstream 100 bases:

>100_bases
GTCGCGCCGCGCAGCGGACGGCCGTTCCTACAATGCCCGAACATTACAAGCGGGACCCGGGCCGCGCCGGGCGCCCCGCA
TGACACGAAGGAGACGTGAC

Downstream 100 bases:

>100_bases
GCGCCGTGCGGCGCGGCGCGATGTTCAGGCGAACGTGTCGACGAGGCCCGCGCGCTGCACGACGGCGGCGCTCGTTCCGG
CCGTCGCGGATGCCGCGGCG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MQYDYIIVGGGSGGSSVAARLADACPDATIALIEAGPHTERNLLVNMPVGIAALVPFKLRTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPSDYDEWAQLGATGWGWQDVLPYFRRAEGNQRGADAWHGADGPLTVSDLRFRNPFSERF
IEAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVAVSRNGRI
ETLGARAEVILSAGAFNSPQLLMCSGIGPAAQLRRHGIAVVHDAPDVGENLIDHIDFIINTRVNSSELVGICLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLDRPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPYSRGTVALASGDA
RDPPLIDPRFFSDSRDLDLLVRGAQAMRRILSQPPLASHGGRELYTHPDQSEAELREAIVAHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVEGLRVVDASVMPTLIGGNTNAPSVMIGERAADFIVAARKGGVPRDQPAATVHGR

Sequences:

>Translated_546_residues
MQYDYIIVGGGSGGSSVAARLADACPDATIALIEAGPHTERNLLVNMPVGIAALVPFKLRTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPSDYDEWAQLGATGWGWQDVLPYFRRAEGNQRGADAWHGADGPLTVSDLRFRNPFSERF
IEAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVAVSRNGRI
ETLGARAEVILSAGAFNSPQLLMCSGIGPAAQLRRHGIAVVHDAPDVGENLIDHIDFIINTRVNSSELVGICLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLDRPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPYSRGTVALASGDA
RDPPLIDPRFFSDSRDLDLLVRGAQAMRRILSQPPLASHGGRELYTHPDQSEAELREAIVAHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVEGLRVVDASVMPTLIGGNTNAPSVMIGERAADFIVAARKGGVPRDQPAATVHGR
>Mature_546_residues
MQYDYIIVGGGSGGSSVAARLADACPDATIALIEAGPHTERNLLVNMPVGIAALVPFKLRTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPSDYDEWAQLGATGWGWQDVLPYFRRAEGNQRGADAWHGADGPLTVSDLRFRNPFSERF
IEAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVAVSRNGRI
ETLGARAEVILSAGAFNSPQLLMCSGIGPAAQLRRHGIAVVHDAPDVGENLIDHIDFIINTRVNSSELVGICLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLDRPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPYSRGTVALASGDA
RDPPLIDPRFFSDSRDLDLLVRGAQAMRRILSQPPLASHGGRELYTHPDQSEAELREAIVAHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVEGLRVVDASVMPTLIGGNTNAPSVMIGERAADFIVAARKGGVPRDQPAATVHGR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=535, Percent_Identity=42.6168224299065, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1786503, Length=535, Percent_Identity=40.5607476635514, Blast_Score=353, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17532301, Length=560, Percent_Identity=36.9642857142857, Blast_Score=346, Evalue=1e-95,
Organism=Drosophila melanogaster, GI45549471, Length=572, Percent_Identity=38.8111888111888, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI45551458, Length=572, Percent_Identity=38.8111888111888, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24642042, Length=571, Percent_Identity=37.8283712784588, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24642048, Length=582, Percent_Identity=36.9415807560137, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24642055, Length=580, Percent_Identity=35.3448275862069, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24642037, Length=577, Percent_Identity=36.9150779896014, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24642039, Length=570, Percent_Identity=35.7894736842105, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24642059, Length=574, Percent_Identity=34.4947735191638, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24642035, Length=581, Percent_Identity=35.9724612736661, Blast_Score=288, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24650267, Length=578, Percent_Identity=35.8131487889273, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI17137792, Length=568, Percent_Identity=34.1549295774648, Blast_Score=277, Evalue=2e-74,
Organism=Drosophila melanogaster, GI18859995, Length=569, Percent_Identity=34.622144112478, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI18859993, Length=577, Percent_Identity=32.9289428076257, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=31.5331010452962, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24642057, Length=583, Percent_Identity=31.7324185248714, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24645930, Length=580, Percent_Identity=28.9655172413793, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58808; Mature: 58808

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYDYIIVGGGSGGSSVAARLADACPDATIALIEAGPHTERNLLVNMPVGIAALVPFKLR
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCEEEEEEEEEE
TNYGYETVPQPGLGGRRGYQPRGRGMGGSSAINAMIYTRGHPSDYDEWAQLGATGWGWQD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNQRGADAWHGADGPLTVSDLRFRNPFSERFIEAAHAAGYPLNDDFNGATQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
EGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVAVSRNGRI
CCCEEEEEECCCCCCEEEEEEEEEECCCCCEEEEECCEEEEECCCCCEEEEEEEECCCCE
ETLGARAEVILSAGAFNSPQLLMCSGIGPAAQLRRHGIAVVHDAPDVGENLIDHIDFIIN
EEECCCEEEEEECCCCCCCCEEEEECCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHEE
TRVNSSELVGICLRGIAKMTPALARYFSSRTGMMTSNVAEAGGFIKSDPSLDRPDLQLHF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCEECCCCCCCCCCCEEEE
CTALVDDHNRKMHWGFGYSLHVCALRPYSRGTVALASGDARDPPLIDPRFFSDSRDLDLL
EEEECCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHH
VRGAQAMRRILSQPPLASHGGRELYTHPDQSEAELREAIVAHADTIYHPVGTCRMGSDAR
HHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCC
AVVDPQLRVRGVEGLRVVDASVMPTLIGGNTNAPSVMIGERAADFIVAARKGGVPRDQPA
EEECCCCEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCHHCEEEECCCCCCCCCCCC
ATVHGR
CCCCCC
>Mature Secondary Structure
MQYDYIIVGGGSGGSSVAARLADACPDATIALIEAGPHTERNLLVNMPVGIAALVPFKLR
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCEEEEEEEEEE
TNYGYETVPQPGLGGRRGYQPRGRGMGGSSAINAMIYTRGHPSDYDEWAQLGATGWGWQD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNQRGADAWHGADGPLTVSDLRFRNPFSERFIEAAHAAGYPLNDDFNGATQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
EGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVGFDGKRAVGVAVSRNGRI
CCCEEEEEECCCCCCEEEEEEEEEECCCCCEEEEECCEEEEECCCCCEEEEEEEECCCCE
ETLGARAEVILSAGAFNSPQLLMCSGIGPAAQLRRHGIAVVHDAPDVGENLIDHIDFIIN
EEECCCEEEEEECCCCCCCCEEEEECCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHEE
TRVNSSELVGICLRGIAKMTPALARYFSSRTGMMTSNVAEAGGFIKSDPSLDRPDLQLHF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCEECCCCCCCCCCCEEEE
CTALVDDHNRKMHWGFGYSLHVCALRPYSRGTVALASGDARDPPLIDPRFFSDSRDLDLL
EEEECCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHH
VRGAQAMRRILSQPPLASHGGRELYTHPDQSEAELREAIVAHADTIYHPVGTCRMGSDAR
HHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCC
AVVDPQLRVRGVEGLRVVDASVMPTLIGGNTNAPSVMIGERAADFIVAARKGGVPRDQPA
EEECCCCEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCHHCEEEECCCCCCCCCCCC
ATVHGR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]