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The map label for this gene is citA [H]

Identifier: 161526210

GI number: 161526210

Start: 3324600

End: 3325907

Strand: Reverse

Name: citA [H]

Synonym: Bmul_3041

Alternate gene names: 161526210

Gene position: 3325907-3324600 (Counterclockwise)

Preceding gene: 161526211

Following gene: 161526209

Centisome position: 96.45

GC content: 68.27

Gene sequence:

>1308_bases
ATGGCGACGATTGCGAATTCAACGAAACCGGTCCGACCCGAGCGTGCGCTGAACCGGCGTGCGGTGGCCGCCGCGGTGAT
CGGCAACGCGCTCGAGTGGTACGACTTCACGGTGTTCAGCTTCATGGCCGTCGTGATCGCCGAGCTGTTCTTTCCGACCT
CGAGCGAATACGGCTCGCTGCTGCTGACCACCGCGACGTTCGGCGTCGCGTTCTTCATGCGTCCGATCGGCGGGATCGTG
CTCGGCCTGTATGCGGACCGCGCGGGCCGCAAGGCCGCGCTGTCGCTCGTGATCCTGCTGATGACGGCCGGCATCTTCCT
GCTCGCGGTCGCGCCGCCGTATGCGGCGATCGGCATCGGCGGCCCGTTGCTGATCGTGTTCGGCCGGCTGCTGCAGGGCT
TCTCCGCCGGCGGCGAGTTCGGCAGCGCGACCGCGCTGCTGATCGAGGCCGCGCCGTTTTCGAAGCGCGGCTTCTACGGC
AGCTGGCAGATGTCGAGCCAGGCCGCCGCGCTGCTGATCGGCGCGGTCGTCGGCGCGGCCGTCACGCGCGGCCTGTCGCC
CGAGGCGCTGCGTGCCTGGGGCTGGCGCGTGCCGTTCATCCTCGGGCTGATGATCGGCCCGATCGGTTTCTACATCCGCC
GCCATCTCGCCGATTCCGAAGCGTTCCTGCACGCGCAGCAGAACGCGCGCCGCGCGACGCTCGGCGACGTGTTCGCGCGC
CACTCGCGCGAAGTGCTCTGCGGGCTCGGCTCGGTGATCGCGCTGACTGTGACGATCTACGTGCTGATCAGCTATCTGCC
GACCTTCGCGGTCAAGCAGCTGAAGCTGCCGTACGCGGAATCGTTCTATGCGGTGATCGCAGGCAATCTGCTGCTGACCG
TGCTGTCGCCGCTCGCCGGTGCATGGTCGGACCGCATCGGCCGCAAGGGGCTCTCGCTCTGGTCGCTGGTGCTGACGCTC
GCGCTGATCTATCCGCTGTTCGCATGGCTCGACGCCGCCCCGAGCATCGGCCGGCTGATCGCGGTGCAGGTCGTGCTGTC
GATCACGCTTGCGGGCTACTACGGGCCGTTCGGCGCGATGATCGCCGAGCTGTTTCCGGCCAACGTGCGCTCGACCGGAC
TGTCGATCGCCTACAACGTCGCGGTGATGCTGTTCGGCGGCTTCGGCCAGTTCATCGTCACGTGGCTGATCAAGGTGACG
GGCACGCCGCTCGCGCCGACGTACTACGTGATGGCGGGGCTCGCGCTGTCGATCGTCGCGGTCGCGTTCGTCCCCGCACG
CAGCGCCGACCTCGACGCGCCTGCATGA

Upstream 100 bases:

>100_bases
AAACGGTTCCGGTGCTTTCTACACTCGCTGCACATCGATGCGCGGGACAACGCGCACGCCGCGCCGTCCGCGACGGCGAA
CTCTCCACGACGAGGCACGC

Downstream 100 bases:

>100_bases
CGCGCATGCGCGCCGATCGTTTCGGTGCGCGCAACGCAGCGCGCGAAATGCGCGAAAACGCCGCAAAAGCGCGGTAAAGG
CGCACCGTGCGGCCCAGCGC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MATIANSTKPVRPERALNRRAVAAAVIGNALEWYDFTVFSFMAVVIAELFFPTSSEYGSLLLTTATFGVAFFMRPIGGIV
LGLYADRAGRKAALSLVILLMTAGIFLLAVAPPYAAIGIGGPLLIVFGRLLQGFSAGGEFGSATALLIEAAPFSKRGFYG
SWQMSSQAAALLIGAVVGAAVTRGLSPEALRAWGWRVPFILGLMIGPIGFYIRRHLADSEAFLHAQQNARRATLGDVFAR
HSREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIAGNLLLTVLSPLAGAWSDRIGRKGLSLWSLVLTL
ALIYPLFAWLDAAPSIGRLIAVQVVLSITLAGYYGPFGAMIAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVT
GTPLAPTYYVMAGLALSIVAVAFVPARSADLDAPA

Sequences:

>Translated_435_residues
MATIANSTKPVRPERALNRRAVAAAVIGNALEWYDFTVFSFMAVVIAELFFPTSSEYGSLLLTTATFGVAFFMRPIGGIV
LGLYADRAGRKAALSLVILLMTAGIFLLAVAPPYAAIGIGGPLLIVFGRLLQGFSAGGEFGSATALLIEAAPFSKRGFYG
SWQMSSQAAALLIGAVVGAAVTRGLSPEALRAWGWRVPFILGLMIGPIGFYIRRHLADSEAFLHAQQNARRATLGDVFAR
HSREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIAGNLLLTVLSPLAGAWSDRIGRKGLSLWSLVLTL
ALIYPLFAWLDAAPSIGRLIAVQVVLSITLAGYYGPFGAMIAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVT
GTPLAPTYYVMAGLALSIVAVAFVPARSADLDAPA
>Mature_434_residues
ATIANSTKPVRPERALNRRAVAAAVIGNALEWYDFTVFSFMAVVIAELFFPTSSEYGSLLLTTATFGVAFFMRPIGGIVL
GLYADRAGRKAALSLVILLMTAGIFLLAVAPPYAAIGIGGPLLIVFGRLLQGFSAGGEFGSATALLIEAAPFSKRGFYGS
WQMSSQAAALLIGAVVGAAVTRGLSPEALRAWGWRVPFILGLMIGPIGFYIRRHLADSEAFLHAQQNARRATLGDVFARH
SREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIAGNLLLTVLSPLAGAWSDRIGRKGLSLWSLVLTLA
LIYPLFAWLDAAPSIGRLIAVQVVLSITLAGYYGPFGAMIAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVTG
TPLAPTYYVMAGLALSIVAVAFVPARSADLDAPA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=407, Percent_Identity=31.6953316953317, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788942, Length=416, Percent_Identity=30.2884615384615, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789941, Length=415, Percent_Identity=32.5301204819277, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788292, Length=433, Percent_Identity=28.8683602771363, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI87082231, Length=250, Percent_Identity=28.4, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082404, Length=431, Percent_Identity=24.8259860788863, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46153; Mature: 46022

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIANSTKPVRPERALNRRAVAAAVIGNALEWYDFTVFSFMAVVIAELFFPTSSEYGSL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
LLTTATFGVAFFMRPIGGIVLGLYADRAGRKAALSLVILLMTAGIFLLAVAPPYAAIGIG
EHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
GPLLIVFGRLLQGFSAGGEFGSATALLIEAAPFSKRGFYGSWQMSSQAAALLIGAVVGAA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VTRGLSPEALRAWGWRVPFILGLMIGPIGFYIRRHLADSEAFLHAQQNARRATLGDVFAR
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
HSREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIAGNLLLTVLSPLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AWSDRIGRKGLSLWSLVLTLALIYPLFAWLDAAPSIGRLIAVQVVLSITLAGYYGPFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
IAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVTGTPLAPTYYVMAGLALSIVA
HHHHCCCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VAFVPARSADLDAPA
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
ATIANSTKPVRPERALNRRAVAAAVIGNALEWYDFTVFSFMAVVIAELFFPTSSEYGSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
LLTTATFGVAFFMRPIGGIVLGLYADRAGRKAALSLVILLMTAGIFLLAVAPPYAAIGIG
EHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
GPLLIVFGRLLQGFSAGGEFGSATALLIEAAPFSKRGFYGSWQMSSQAAALLIGAVVGAA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VTRGLSPEALRAWGWRVPFILGLMIGPIGFYIRRHLADSEAFLHAQQNARRATLGDVFAR
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
HSREVLCGLGSVIALTVTIYVLISYLPTFAVKQLKLPYAESFYAVIAGNLLLTVLSPLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AWSDRIGRKGLSLWSLVLTLALIYPLFAWLDAAPSIGRLIAVQVVLSITLAGYYGPFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHH
IAELFPANVRSTGLSIAYNVAVMLFGGFGQFIVTWLIKVTGTPLAPTYYVMAGLALSIVA
HHHHCCCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VAFVPARSADLDAPA
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]