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The map label for this gene is flgJ [H]

Identifier: 161526181

GI number: 161526181

Start: 3294665

End: 3295624

Strand: Reverse

Name: flgJ [H]

Synonym: Bmul_3012

Alternate gene names: 161526181

Gene position: 3295624-3294665 (Counterclockwise)

Preceding gene: 161526182

Following gene: 161526180

Centisome position: 95.57

GC content: 69.58

Gene sequence:

>960_bases
ATGGCGAATTTCCCCGGAGCCACCGACCTCACGCAGCGCTTCGCGCTCGACGTGCAGGGCTTCGACGCGCTGCGCGCGCA
GGCGAAACAGTCGCCGCAGGGCGGCGCGAAGGCGGTCGCAGGCCAGTTCGACGCGATGTTCACGCAGATGATGCTGAAGA
GCATGCGCGACGCGACGCCCGAAGGCGGGCTGTTCGACTCGCATACGTCGAAGATGTACACGTCGATGCTCGACCAGCAG
CTCGCGCAGCAGATGTCGTCGCGCGGCATCGGCGTCGCCGACGCGCTGATGAAGCAATTGATGCGCAACGCCGGGCAGGG
CGCGGGCGCGGCGGCCGACCTCGGCGCCGGCTTCGGCGCGGCCGGCAACGAAGGCAGCCTCGCCGCGATGAGCGCGATGG
CGAACGCCTATGCGAACGCGGCCGCGAACAACGGCGGCCTGTCGCGCGCGCGCGGCTATTCGGCCGGCAGCGCGCTGACG
CCGCCGCTGAAGGGCGCGACCGGTGCGCCGGACGCGGACGCGTTCGTCGACCGCCTCGCCGGCCCCGCACAGGCCGCGAG
CGCGGCGACCGGCATTCCGGCGCGCTTCATCGTCGGCCAGGCGGCGCTCGAATCGGGCTGGGGCAAGCGCGAGATCCGCG
CGGCCGACGGCTCGACCAGCTACAACGTGTTCGGCATCAAGGCGACGCGCGGCTGGACCGGCCGCACGGTATCGGCGCTG
ACGACCGAATACGTGAACGGCACGCCGCGGCGTGTCGTCGCGAAGTTCCGCGCGTACGATTCGTACGAGCATGCGATGAC
CGACTACGCGAACCTGCTGAAGAACAATCCGCGCTATGCGGGCGTGCTGAGCGCGAGCCGCAGCGTCGAGGGTTTTGCGC
AGGGAATGCAGAAGGCCGGCTATGCGACGGACCCGAACTACGCGAAAAAGCTGATCTCGATCATGCAGCAGATCGGCTGA

Upstream 100 bases:

>100_bases
GGCGCTGAACACGCTCGGCGCGACGCCGGCCGACCTGATGTCGATCCTGCAGGCGATGAAGGCGGCCGGTGCGCTGCGCG
CGGACCTGGAGATCATCTAA

Downstream 100 bases:

>100_bases
CGAATCGCTGACGCCTTGGCCTCCGACGGACCGCGCCCGGCACCCCGCCGCGCGCGGTTTCAACGTTTGCGCGAAAAAAA
TCGGCAAATCGATCTAAACT

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MANFPGATDLTQRFALDVQGFDALRAQAKQSPQGGAKAVAGQFDAMFTQMMLKSMRDATPEGGLFDSHTSKMYTSMLDQQ
LAQQMSSRGIGVADALMKQLMRNAGQGAGAAADLGAGFGAAGNEGSLAAMSAMANAYANAAANNGGLSRARGYSAGSALT
PPLKGATGAPDADAFVDRLAGPAQAASAATGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKATRGWTGRTVSAL
TTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAQGMQKAGYATDPNYAKKLISIMQQIG

Sequences:

>Translated_319_residues
MANFPGATDLTQRFALDVQGFDALRAQAKQSPQGGAKAVAGQFDAMFTQMMLKSMRDATPEGGLFDSHTSKMYTSMLDQQ
LAQQMSSRGIGVADALMKQLMRNAGQGAGAAADLGAGFGAAGNEGSLAAMSAMANAYANAAANNGGLSRARGYSAGSALT
PPLKGATGAPDADAFVDRLAGPAQAASAATGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKATRGWTGRTVSAL
TTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAQGMQKAGYATDPNYAKKLISIMQQIG
>Mature_318_residues
ANFPGATDLTQRFALDVQGFDALRAQAKQSPQGGAKAVAGQFDAMFTQMMLKSMRDATPEGGLFDSHTSKMYTSMLDQQL
AQQMSSRGIGVADALMKQLMRNAGQGAGAAADLGAGFGAAGNEGSLAAMSAMANAYANAAANNGGLSRARGYSAGSALTP
PLKGATGAPDADAFVDRLAGPAQAASAATGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKATRGWTGRTVSALT
TEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAQGMQKAGYATDPNYAKKLISIMQQIG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=307, Percent_Identity=41.3680781758958, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33114; Mature: 32982

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFPGATDLTQRFALDVQGFDALRAQAKQSPQGGAKAVAGQFDAMFTQMMLKSMRDATP
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EGGLFDSHTSKMYTSMLDQQLAQQMSSRGIGVADALMKQLMRNAGQGAGAAADLGAGFGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
AGNEGSLAAMSAMANAYANAAANNGGLSRARGYSAGSALTPPLKGATGAPDADAFVDRLA
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
GPAQAASAATGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKATRGWTGRTVSAL
CHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCCCHHHHH
TTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAQGMQKAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCC
YATDPNYAKKLISIMQQIG
CCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ANFPGATDLTQRFALDVQGFDALRAQAKQSPQGGAKAVAGQFDAMFTQMMLKSMRDATP
CCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EGGLFDSHTSKMYTSMLDQQLAQQMSSRGIGVADALMKQLMRNAGQGAGAAADLGAGFGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
AGNEGSLAAMSAMANAYANAAANNGGLSRARGYSAGSALTPPLKGATGAPDADAFVDRLA
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
GPAQAASAATGIPARFIVGQAALESGWGKREIRAADGSTSYNVFGIKATRGWTGRTVSAL
CHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCCCHHHHH
TTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYAGVLSASRSVEGFAQGMQKAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCC
YATDPNYAKKLISIMQQIG
CCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 2193164; 10049388 [H]