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The map label for this gene is gcvA [H]

Identifier: 161526176

GI number: 161526176

Start: 3287612

End: 3288496

Strand: Direct

Name: gcvA [H]

Synonym: Bmul_3007

Alternate gene names: 161526176

Gene position: 3287612-3288496 (Clockwise)

Preceding gene: 161526173

Following gene: 161526190

Centisome position: 95.34

GC content: 70.73

Gene sequence:

>885_bases
ATGCGTCGCGACCTCCCGCCCTTCTCCGCGCTTCGGGCCTTTGAAGCCGCGGCCCGCCATGAAAGCTTCAGCGCTGCCGG
CGACGAGCTGCATGTGACCCACGGTGCCATCAGCCGGCAGATCGCCGGCTTCGAGGCATGGCTCGGCAAGCCCGTGTTTC
ACCGCTACGGCAAGCGCGTGAAGCTCACCGACGAAGGCCGCCGCTATCTCGACACCGTGCGCGCCGCGTTCGACAGCATC
GCGCTCGCGACCGAGCAGCTGCGCCATACCGGCACCGCGCGCGTGCTGCGCATCAATGCACTGCCGACCTTCGCGATGAA
ATGGCTGCTGCCGCGGCTGTCGCGGTTCCAGCGCGACGTGCCGAACGTCGAGCTGAAGCTGTCGACGTCGAATGCGCCGC
TCGACACGCTCGACGGTTTCGACGTCGCGATCCGGCGCGGCCCCGGCCACTGGCCGGCATGCACGAGCGGCCGCTTCCTC
GACGAAAGCGTGATTCCGGTCTGCAGCCCGGCGCTGCTCAAGCGCGCGCCGATCGCACGCGCGGACGATCTCGCGCGGCA
CGTGCTGCTGCATTCGGATACGCGGCCGGAAGGCTGGCGCGACTGGTTCGCGGCGGCCGGCGTGACGATGAAGGCGCGCA
AGCGGCAGTCGTTCGACCACTTCTATCTCGCGCTGCAGGCGGCCGTCGACGGGCTCGGCGTCGCGCTCGGCCCGCTGCCG
CTGATCGACGACGAACTCGCGAGCGGCCGCCTCGTGATGCCGCTCGCGGGCCCGCGCATCGCGACGCGCAGCTACTGGTG
GATCGCGCCGCGCGCGCCGGCGGACGATCCGCTCGTCGCGCAGTTCTGCGCGTGGCTGGAAGCGCAGTCGAACGTCGGCG
CGTAG

Upstream 100 bases:

>100_bases
GGCATGGGGTTCCTCATCGAAAGTAGGCCGAGGTTACCGCCGATCCGCGCGGCGGCAAAACGATTTTATCGACGCGCCTT
TGTGATCTAGAATCACAAGC

Downstream 100 bases:

>100_bases
TACCGCGCGGTTCGACGAAGCAAGCGGTGCGGGCGGCAGGACAGCGCCACACCGCGCCGCCCATGCGGCCCGCGCCGCGC
GCGGACGGCCTTGCACCGCC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRRDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSI
ALATEQLRHTGTARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPACTSGRFL
DESVIPVCSPALLKRAPIARADDLARHVLLHSDTRPEGWRDWFAAAGVTMKARKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLAGPRIATRSYWWIAPRAPADDPLVAQFCAWLEAQSNVGA

Sequences:

>Translated_294_residues
MRRDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSI
ALATEQLRHTGTARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPACTSGRFL
DESVIPVCSPALLKRAPIARADDLARHVLLHSDTRPEGWRDWFAAAGVTMKARKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLAGPRIATRSYWWIAPRAPADDPLVAQFCAWLEAQSNVGA
>Mature_294_residues
MRRDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSI
ALATEQLRHTGTARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPACTSGRFL
DESVIPVCSPALLKRAPIARADDLARHVLLHSDTRPEGWRDWFAAAGVTMKARKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLAGPRIATRSYWWIAPRAPADDPLVAQFCAWLEAQSNVGA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=35.1535836177474, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=29.3103448275862, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=29.7945205479452, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787128, Length=151, Percent_Identity=34.4370860927152, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786401, Length=252, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=30.8641975308642, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=26.2548262548263, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32531; Mature: 32531

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLDTVRAAFDSIALATEQLRHTGTARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPACTSGRFLDESVIPVCSPALLKRAPIAR
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVTMKARKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLAGPRIATRSYWWIAPRAPADDPLVAQFCAWLEAQSNVGA
CCCHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLDTVRAAFDSIALATEQLRHTGTARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPACTSGRFLDESVIPVCSPALLKRAPIAR
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVTMKARKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLAGPRIATRSYWWIAPRAPADDPLVAQFCAWLEAQSNVGA
CCCHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]